Use of natural antioxidants in in vitro mammalian embryo production

Detalhes bibliográficos
Autor(a) principal: Santos, Maria Valéria de Oliveira
Data de Publicação: 2018
Outros Autores: Borges, Alana Azevedo, Queiroz Neta, Luiza Bento de, Bertini, Luciana Medeiros, Pereira, Alexsandra Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/30471
Resumo: In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology.
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spelling Use of natural antioxidants in in vitro mammalian embryo productionUso de antioxidantes naturais na produção in vitro de embriões de mamíferosCulture mediumNatural bioactiveOxidative stressReproduction.Bioativo naturalEstresse oxidativoMeio de cultivoReprodução.In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology.A produção in vitro de embriões (PIVE) contribui para os aspectos quantitativos e qualitativos da reprodução animal. Contudo, fatores inerentes da técnica, como o estresse oxidativo, podem influenciar negativamente o resultado e isso pode prejudicar o metabolismo celular, diminuindo assim as taxas de desenvolvimento in vitro, e exigindo a suplementação do meio de cultivo com antioxidantes. Neste contexto, os compostos de origem natural com essa propriedade têm se destacado devido aos resultados positivos obtidos em diferentes estágios da PIVE. Assim, esta revisão pretende apresentar os resultados obtidos usando antioxidantes naturais para minimizar os efeitos do estresse oxidativo em gametas e embriões. Uma variedade de substâncias isoladas e de misturas naturais (óleos essenciais e extratos) tem sido estudada para a suplementação de meios de PIVE, nas etapas de maturação in vitro, capacitação espermática, fecundação in vitro e desenvolvimento in vitro de embriões em diferentes espécies de mamíferos. Geralmente, os efeitos benéficos são observados de acordo com a concentração utilizada, demostrando assim o potencial positivo de vários antioxidantes naturais. Portanto, os principais desafios para o uso desses compostos como antioxidantes durante a PIVE incluem provar sua eficiência contra os radicais livres e determinar a melhor concentração em cada etapa. Além disso, a compreensão dos mecanismos de ação de tais antioxidantes é crucial para estabelecer o uso na biotecnologia de PIVE.UEL2018-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRevisão de Literaturaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3047110.5433/1679-0359.2018v39n1p431Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 431-444Semina: Ciências Agrárias; v. 39 n. 1 (2018); 431-4441679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/30471/23077Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSantos, Maria Valéria de OliveiraBorges, Alana AzevedoQueiroz Neta, Luiza Bento deBertini, Luciana MedeirosPereira, Alexsandra Fernandes2022-10-21T12:34:06Zoai:ojs.pkp.sfu.ca:article/30471Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T12:34:06Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Use of natural antioxidants in in vitro mammalian embryo production
Uso de antioxidantes naturais na produção in vitro de embriões de mamíferos
title Use of natural antioxidants in in vitro mammalian embryo production
spellingShingle Use of natural antioxidants in in vitro mammalian embryo production
Santos, Maria Valéria de Oliveira
Culture medium
Natural bioactive
Oxidative stress
Reproduction.
Bioativo natural
Estresse oxidativo
Meio de cultivo
Reprodução.
title_short Use of natural antioxidants in in vitro mammalian embryo production
title_full Use of natural antioxidants in in vitro mammalian embryo production
title_fullStr Use of natural antioxidants in in vitro mammalian embryo production
title_full_unstemmed Use of natural antioxidants in in vitro mammalian embryo production
title_sort Use of natural antioxidants in in vitro mammalian embryo production
author Santos, Maria Valéria de Oliveira
author_facet Santos, Maria Valéria de Oliveira
Borges, Alana Azevedo
Queiroz Neta, Luiza Bento de
Bertini, Luciana Medeiros
Pereira, Alexsandra Fernandes
author_role author
author2 Borges, Alana Azevedo
Queiroz Neta, Luiza Bento de
Bertini, Luciana Medeiros
Pereira, Alexsandra Fernandes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Maria Valéria de Oliveira
Borges, Alana Azevedo
Queiroz Neta, Luiza Bento de
Bertini, Luciana Medeiros
Pereira, Alexsandra Fernandes
dc.subject.por.fl_str_mv Culture medium
Natural bioactive
Oxidative stress
Reproduction.
Bioativo natural
Estresse oxidativo
Meio de cultivo
Reprodução.
topic Culture medium
Natural bioactive
Oxidative stress
Reproduction.
Bioativo natural
Estresse oxidativo
Meio de cultivo
Reprodução.
description In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Revisão de Literatura
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/30471
10.5433/1679-0359.2018v39n1p431
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/30471
identifier_str_mv 10.5433/1679-0359.2018v39n1p431
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/30471/23077
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 431-444
Semina: Ciências Agrárias; v. 39 n. 1 (2018); 431-444
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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