Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2545 |
Resumo: | The aim of this work was to evaluate the antifungical, antibacterial and phytoalexins elicitors activities of hydrolates, for using in the alternative control of plant diseases. The hydrolates of Helietta apiculata (HA), Conyza canadensis (CC) and Cymbopogon nardus (CN) were used in the concentrations of 1, 5, 10, 15, 20 and 25%. In the phytoalexins assay, sorghum etiolated mesocotyls were used. The antibacterial effect was evaluated on the growth of the bacterium Xanthomonas campestris pv. campestris in liquid nutrient medium and antibiotic (oxytetraciclin 22.5 mg/L + streptomycin 225 mg/L) was used as control treatment. The antifungical effect was evaluated on the micelial growth, esporulation, conidia germination and development of germinative tubes of Alternaria brassicae. The fungicide azoxystrobin (0.08 g a.i./ L) was used as control treatment. There was increment in the phytoalexins synthesis with the increase in hydrolates concentration. The better result was obtained with CN that promoted increase 4.3 times larger in relation to the value of the control treatment with water, followed by HA (2.5 times) and CC (2.1 times). Similar results were obtained to the antibacterial activity with the following inhibition results (%) in the development of the bacterium: CN: 29.8, HA: 14.9, CC: 14.6 and antibiotic: 97.7. The main antifungical effect was observed on the development of the germinative tubes. Hydrolates of CC and HA showed inhibition of up to 69.2 and 56.2%, respectively, being similar to the fungicide azoxystrobin. The hydrolate of CN did not show antifungical effect. These results indicate the presence of phytoalexins elicitors, and antibacterial and antifungical compounds in those hydrolates, however in low concentrations. |
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Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plantsAtividades antibacteriana, antifúngica e indutora de fitoalexinas de hidrolatos de plantas medicinaisAntimicrobialHelietta apiculataConyza canadensisCymbopogon nardusAlternative control.AntimicrobianoHelietta apiculataConyza canadensiCymbopogon nardusControle.The aim of this work was to evaluate the antifungical, antibacterial and phytoalexins elicitors activities of hydrolates, for using in the alternative control of plant diseases. The hydrolates of Helietta apiculata (HA), Conyza canadensis (CC) and Cymbopogon nardus (CN) were used in the concentrations of 1, 5, 10, 15, 20 and 25%. In the phytoalexins assay, sorghum etiolated mesocotyls were used. The antibacterial effect was evaluated on the growth of the bacterium Xanthomonas campestris pv. campestris in liquid nutrient medium and antibiotic (oxytetraciclin 22.5 mg/L + streptomycin 225 mg/L) was used as control treatment. The antifungical effect was evaluated on the micelial growth, esporulation, conidia germination and development of germinative tubes of Alternaria brassicae. The fungicide azoxystrobin (0.08 g a.i./ L) was used as control treatment. There was increment in the phytoalexins synthesis with the increase in hydrolates concentration. The better result was obtained with CN that promoted increase 4.3 times larger in relation to the value of the control treatment with water, followed by HA (2.5 times) and CC (2.1 times). Similar results were obtained to the antibacterial activity with the following inhibition results (%) in the development of the bacterium: CN: 29.8, HA: 14.9, CC: 14.6 and antibiotic: 97.7. The main antifungical effect was observed on the development of the germinative tubes. Hydrolates of CC and HA showed inhibition of up to 69.2 and 56.2%, respectively, being similar to the fungicide azoxystrobin. The hydrolate of CN did not show antifungical effect. These results indicate the presence of phytoalexins elicitors, and antibacterial and antifungical compounds in those hydrolates, however in low concentrations.O objetivo do trabalho foi avaliar a atividade como antifúngico, antibacteriano e indutor da produção de fitoalexinas dos hidrolatos de Helietta apiculata (canela-de-veado) (HA), Conyza canadensis (buva) (CC) e Cymbopogon nardus (citronela) (CN) nas concentrações de 1, 5, 10, 15, 20 e 25%, buscando seu uso no controle alternativo de doenças em plantas. Para o ensaio de fitoalexinas foram utilizados mesocótilos estiolados de sorgo. Para o efeito antibacteriano foi avaliado o crescimento da bactéria Xanthomonas campestris pv. campestris em meio caldo nutriente suplementado com os tratamentos, tendo como testemunha mistura de antibióticos (22,5 mg/L de oxitetraciclina + 225 mg/L de estreptomicina). O efeito antifúngico foi avaliado através da mensuração do crescimento vegetativo, esporulação, germinação de esporos e desenvolvimento do tubo germinativo de Alternaria brassicae, sendo o fungicida azoxystrobin (0,08 g i.a./L) usado como controle. Foi observado acréscimo na síntese de fitoalexinas com o aumento na concentração dos hidrolatos, sendo o melhor resultado obtido com CN, o qual promoveu aumento de cerca de 4,3 vezes mais, seguido de HA (2,5 vezes) e CC (2,1 vezes), em relação a testemunha água. Resultados semelhantes foram obtidos para crescimento bacteriano com os seguintes resultados de inibição (em %) no desenvolvimento da bactéria: CN: 29,8, HA: 14,9, CC: 14,6 e antibióticos: 97,7. Com relação à ação antifúngica foi observada inibição do desenvolvimento dos tubos germinativos, sendo que os hidrolatos de CC e HA inibiram 69,2 e 56,2%, respectivamente, resultado semelhante ao fungicida. O hidrolato de CN não apresentou efeito antifúngico. Estes resultados indicam a presença de compostos indutores de fitoalexinas, antibacterianos e antifúngicos nesses hidrolatos, porém em baixas concentrações.UEL2007-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/254510.5433/1679-0359.2007v28n1p29Semina: Ciências Agrárias; Vol. 28 No. 1 (2007); 29-38Semina: Ciências Agrárias; v. 28 n. 1 (2007); 29-381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2545/2181Franzener, GilmarMartinez-Franzener, Alexandra da SilvaStangarlin, José RenatoCzepak, Márcio PauloSchwan-Estrada, Kátia Regina FreitasCruz, Maria Eugênia Silvainfo:eu-repo/semantics/openAccess2009-07-16T20:13:26Zoai:ojs.pkp.sfu.ca:article/2545Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-07-16T20:13:26Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants Atividades antibacteriana, antifúngica e indutora de fitoalexinas de hidrolatos de plantas medicinais |
title |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
spellingShingle |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants Franzener, Gilmar Antimicrobial Helietta apiculata Conyza canadensis Cymbopogon nardus Alternative control. Antimicrobiano Helietta apiculata Conyza canadensi Cymbopogon nardus Controle. |
title_short |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
title_full |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
title_fullStr |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
title_full_unstemmed |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
title_sort |
Antibacterial, antifungal and phytoalexins induction activities of hydrolates of medicinal plants |
author |
Franzener, Gilmar |
author_facet |
Franzener, Gilmar Martinez-Franzener, Alexandra da Silva Stangarlin, José Renato Czepak, Márcio Paulo Schwan-Estrada, Kátia Regina Freitas Cruz, Maria Eugênia Silva |
author_role |
author |
author2 |
Martinez-Franzener, Alexandra da Silva Stangarlin, José Renato Czepak, Márcio Paulo Schwan-Estrada, Kátia Regina Freitas Cruz, Maria Eugênia Silva |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Franzener, Gilmar Martinez-Franzener, Alexandra da Silva Stangarlin, José Renato Czepak, Márcio Paulo Schwan-Estrada, Kátia Regina Freitas Cruz, Maria Eugênia Silva |
dc.subject.por.fl_str_mv |
Antimicrobial Helietta apiculata Conyza canadensis Cymbopogon nardus Alternative control. Antimicrobiano Helietta apiculata Conyza canadensi Cymbopogon nardus Controle. |
topic |
Antimicrobial Helietta apiculata Conyza canadensis Cymbopogon nardus Alternative control. Antimicrobiano Helietta apiculata Conyza canadensi Cymbopogon nardus Controle. |
description |
The aim of this work was to evaluate the antifungical, antibacterial and phytoalexins elicitors activities of hydrolates, for using in the alternative control of plant diseases. The hydrolates of Helietta apiculata (HA), Conyza canadensis (CC) and Cymbopogon nardus (CN) were used in the concentrations of 1, 5, 10, 15, 20 and 25%. In the phytoalexins assay, sorghum etiolated mesocotyls were used. The antibacterial effect was evaluated on the growth of the bacterium Xanthomonas campestris pv. campestris in liquid nutrient medium and antibiotic (oxytetraciclin 22.5 mg/L + streptomycin 225 mg/L) was used as control treatment. The antifungical effect was evaluated on the micelial growth, esporulation, conidia germination and development of germinative tubes of Alternaria brassicae. The fungicide azoxystrobin (0.08 g a.i./ L) was used as control treatment. There was increment in the phytoalexins synthesis with the increase in hydrolates concentration. The better result was obtained with CN that promoted increase 4.3 times larger in relation to the value of the control treatment with water, followed by HA (2.5 times) and CC (2.1 times). Similar results were obtained to the antibacterial activity with the following inhibition results (%) in the development of the bacterium: CN: 29.8, HA: 14.9, CC: 14.6 and antibiotic: 97.7. The main antifungical effect was observed on the development of the germinative tubes. Hydrolates of CC and HA showed inhibition of up to 69.2 and 56.2%, respectively, being similar to the fungicide azoxystrobin. The hydrolate of CN did not show antifungical effect. These results indicate the presence of phytoalexins elicitors, and antibacterial and antifungical compounds in those hydrolates, however in low concentrations. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-07-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2545 10.5433/1679-0359.2007v28n1p29 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2545 |
identifier_str_mv |
10.5433/1679-0359.2007v28n1p29 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2545/2181 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 28 No. 1 (2007); 29-38 Semina: Ciências Agrárias; v. 28 n. 1 (2007); 29-38 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306058504601600 |