Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil

Detalhes bibliográficos
Autor(a) principal: Ferreira, Maria Regina
Data de Publicação: 2020
Outros Autores: Souza, Aline Moreira de, Campos, Sabrina Destri Emmerick, Cunha, Nathalie Costa da, Silva, Beatriz Teixeira Gomes da, Silva, Nathalia Xavier da, Almosny, Nádia Regina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343
Resumo: Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.
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spelling Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, BrazilPesquisa molecular sobre a presença de hemoparasitos transmitidos por carrapatos em cães e carrapatos presentes no ambiente de Mata Atlântica no Estado do Rio de Janeiro, BrasilBabesia vogeliRhipicephalus sanguineusRainforest biomePolymerase chain reaction.Babesia vogeliRhipicephalus sanguineusBioma de Mata AtlânticaReação em cadeia da polimerase.Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.Doenças transmitidas por carrapatos são capazes de causar impacto significativo na medicina humana e veterinária em todo o mundo. O Parque Estadual Serra da Tiririca (PESET) é uma Reserva ambiental de mata atlântica que abriga uma grande variedade de espécies de animais silvestres, porém, vem sofrendo com a ação de atividades humanas não autorizadas, como as construções irregulares e introdução de animais domésticos. O objetivo deste trabalho foi a detecção de piroplasmas e Hepatozoon spp., em cães e carrapatos. Foram coletadas amostras sanguíneas de 124 caninos assintomáticos e de 88 carrapatos Rhipicephalus sanguineus coletados de animais que habitavam o PESET. Técnicas moleculares tendo como alvo fragmentos do gene 18s rRNA de piroplasmas e de Hepatozoon spp. foram empregadas em todas as amostras obtidas, sendo encontrada uma amostra de sangue (0.81%) positiva para Babesia vogeli. Todos os cães foram negativos à pesquisa de Hepatozoon spp. e nenhuma amostra de carrapato apresentou resultado positivo para ambos os ensaios de PCR. Apesar da baixa frequência de infecção por B. vogeli encontrada nos caninos que habitam o PESET, nós alertamos para a possibilidade de que cães parasitados possam servir de fonte de transmissão para novos vetores, outros cães e até canídeos silvestres, que façam parte da fauna nativa do parque.UEL2020-03-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3534310.5433/1679-0359.2020v41n2p689Semina: Ciências Agrárias; Vol. 41 No. 2 (2020); 689-696Semina: Ciências Agrárias; v. 41 n. 2 (2020); 689-6961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343/26881Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFerreira, Maria ReginaSouza, Aline Moreira deCampos, Sabrina Destri EmmerickCunha, Nathalie Costa daSilva, Beatriz Teixeira Gomes daSilva, Nathalia Xavier daAlmosny, Nádia Regina Pereira2022-10-10T13:58:18Zoai:ojs.pkp.sfu.ca:article/35343Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T13:58:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
Pesquisa molecular sobre a presença de hemoparasitos transmitidos por carrapatos em cães e carrapatos presentes no ambiente de Mata Atlântica no Estado do Rio de Janeiro, Brasil
title Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
spellingShingle Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
Ferreira, Maria Regina
Babesia vogeli
Rhipicephalus sanguineus
Rainforest biome
Polymerase chain reaction.
Babesia vogeli
Rhipicephalus sanguineus
Bioma de Mata Atlântica
Reação em cadeia da polimerase.
title_short Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
title_full Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
title_fullStr Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
title_full_unstemmed Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
title_sort Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
author Ferreira, Maria Regina
author_facet Ferreira, Maria Regina
Souza, Aline Moreira de
Campos, Sabrina Destri Emmerick
Cunha, Nathalie Costa da
Silva, Beatriz Teixeira Gomes da
Silva, Nathalia Xavier da
Almosny, Nádia Regina Pereira
author_role author
author2 Souza, Aline Moreira de
Campos, Sabrina Destri Emmerick
Cunha, Nathalie Costa da
Silva, Beatriz Teixeira Gomes da
Silva, Nathalia Xavier da
Almosny, Nádia Regina Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Maria Regina
Souza, Aline Moreira de
Campos, Sabrina Destri Emmerick
Cunha, Nathalie Costa da
Silva, Beatriz Teixeira Gomes da
Silva, Nathalia Xavier da
Almosny, Nádia Regina Pereira
dc.subject.por.fl_str_mv Babesia vogeli
Rhipicephalus sanguineus
Rainforest biome
Polymerase chain reaction.
Babesia vogeli
Rhipicephalus sanguineus
Bioma de Mata Atlântica
Reação em cadeia da polimerase.
topic Babesia vogeli
Rhipicephalus sanguineus
Rainforest biome
Polymerase chain reaction.
Babesia vogeli
Rhipicephalus sanguineus
Bioma de Mata Atlântica
Reação em cadeia da polimerase.
description Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343
10.5433/1679-0359.2020v41n2p689
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343
identifier_str_mv 10.5433/1679-0359.2020v41n2p689
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343/26881
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 2 (2020); 689-696
Semina: Ciências Agrárias; v. 41 n. 2 (2020); 689-696
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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