Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343 |
Resumo: | Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park. |
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Semina. Ciências Agrárias (Online) |
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Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, BrazilPesquisa molecular sobre a presença de hemoparasitos transmitidos por carrapatos em cães e carrapatos presentes no ambiente de Mata Atlântica no Estado do Rio de Janeiro, BrasilBabesia vogeliRhipicephalus sanguineusRainforest biomePolymerase chain reaction.Babesia vogeliRhipicephalus sanguineusBioma de Mata AtlânticaReação em cadeia da polimerase.Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.Doenças transmitidas por carrapatos são capazes de causar impacto significativo na medicina humana e veterinária em todo o mundo. O Parque Estadual Serra da Tiririca (PESET) é uma Reserva ambiental de mata atlântica que abriga uma grande variedade de espécies de animais silvestres, porém, vem sofrendo com a ação de atividades humanas não autorizadas, como as construções irregulares e introdução de animais domésticos. O objetivo deste trabalho foi a detecção de piroplasmas e Hepatozoon spp., em cães e carrapatos. Foram coletadas amostras sanguíneas de 124 caninos assintomáticos e de 88 carrapatos Rhipicephalus sanguineus coletados de animais que habitavam o PESET. Técnicas moleculares tendo como alvo fragmentos do gene 18s rRNA de piroplasmas e de Hepatozoon spp. foram empregadas em todas as amostras obtidas, sendo encontrada uma amostra de sangue (0.81%) positiva para Babesia vogeli. Todos os cães foram negativos à pesquisa de Hepatozoon spp. e nenhuma amostra de carrapato apresentou resultado positivo para ambos os ensaios de PCR. Apesar da baixa frequência de infecção por B. vogeli encontrada nos caninos que habitam o PESET, nós alertamos para a possibilidade de que cães parasitados possam servir de fonte de transmissão para novos vetores, outros cães e até canídeos silvestres, que façam parte da fauna nativa do parque.UEL2020-03-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3534310.5433/1679-0359.2020v41n2p689Semina: Ciências Agrárias; Vol. 41 No. 2 (2020); 689-696Semina: Ciências Agrárias; v. 41 n. 2 (2020); 689-6961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343/26881Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFerreira, Maria ReginaSouza, Aline Moreira deCampos, Sabrina Destri EmmerickCunha, Nathalie Costa daSilva, Beatriz Teixeira Gomes daSilva, Nathalia Xavier daAlmosny, Nádia Regina Pereira2022-10-10T13:58:18Zoai:ojs.pkp.sfu.ca:article/35343Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T13:58:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil Pesquisa molecular sobre a presença de hemoparasitos transmitidos por carrapatos em cães e carrapatos presentes no ambiente de Mata Atlântica no Estado do Rio de Janeiro, Brasil |
title |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
spellingShingle |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil Ferreira, Maria Regina Babesia vogeli Rhipicephalus sanguineus Rainforest biome Polymerase chain reaction. Babesia vogeli Rhipicephalus sanguineus Bioma de Mata Atlântica Reação em cadeia da polimerase. |
title_short |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
title_full |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
title_fullStr |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
title_full_unstemmed |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
title_sort |
Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil |
author |
Ferreira, Maria Regina |
author_facet |
Ferreira, Maria Regina Souza, Aline Moreira de Campos, Sabrina Destri Emmerick Cunha, Nathalie Costa da Silva, Beatriz Teixeira Gomes da Silva, Nathalia Xavier da Almosny, Nádia Regina Pereira |
author_role |
author |
author2 |
Souza, Aline Moreira de Campos, Sabrina Destri Emmerick Cunha, Nathalie Costa da Silva, Beatriz Teixeira Gomes da Silva, Nathalia Xavier da Almosny, Nádia Regina Pereira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Maria Regina Souza, Aline Moreira de Campos, Sabrina Destri Emmerick Cunha, Nathalie Costa da Silva, Beatriz Teixeira Gomes da Silva, Nathalia Xavier da Almosny, Nádia Regina Pereira |
dc.subject.por.fl_str_mv |
Babesia vogeli Rhipicephalus sanguineus Rainforest biome Polymerase chain reaction. Babesia vogeli Rhipicephalus sanguineus Bioma de Mata Atlântica Reação em cadeia da polimerase. |
topic |
Babesia vogeli Rhipicephalus sanguineus Rainforest biome Polymerase chain reaction. Babesia vogeli Rhipicephalus sanguineus Bioma de Mata Atlântica Reação em cadeia da polimerase. |
description |
Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343 10.5433/1679-0359.2020v41n2p689 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343 |
identifier_str_mv |
10.5433/1679-0359.2020v41n2p689 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35343/26881 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 2 (2020); 689-696 Semina: Ciências Agrárias; v. 41 n. 2 (2020); 689-696 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081026965504 |