Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry

Detalhes bibliográficos
Autor(a) principal: Anai, Letícia Abrahão
Data de Publicação: 2013
Outros Autores: Coleta, Flávia Eiras Dela, Munhoz, Thiago Demarchi, Nogueira, Andressa Francisca Silva, Semolin, Lívia Maria Souza, Vieira, Manuela Cristina, Santana, Aureo Evangelista
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13301
Resumo: Lymphoma is the most common hematopoietic tumor in dogs and one of the malignant tumors with higher occurrence in this species. It is a great experimental model due to its resemblance with the non- Hodgkin lymphoma in humans. Considering the importance of the overall changes that result from to this kind of neoplastic tumor and those due to polichemotherapy this study aimed to evaluate the absolute leukon count and the total count of CD45+ cells in the blood of 25 dogs with lymphoma. Findings were crosschecked since diagnose and then once weekly during the first eight sessions of the Madison-Wisconsin chemotherapic protocol. Total granulocyte, lymphocyte and monocyte counts obtained from a conventional automatic counter and by flow cytometry were compared. Results did not reveal statistically significant changes between the two techniques. 
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spelling Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometryAvaliação leucocitária de cães com linfoma submetidos ao protocolo de Madison-Wisconsin pela técnica convencional e citometria de fluxoCD45LymphomaDog.CD45LinfomaCão.Lymphoma is the most common hematopoietic tumor in dogs and one of the malignant tumors with higher occurrence in this species. It is a great experimental model due to its resemblance with the non- Hodgkin lymphoma in humans. Considering the importance of the overall changes that result from to this kind of neoplastic tumor and those due to polichemotherapy this study aimed to evaluate the absolute leukon count and the total count of CD45+ cells in the blood of 25 dogs with lymphoma. Findings were crosschecked since diagnose and then once weekly during the first eight sessions of the Madison-Wisconsin chemotherapic protocol. Total granulocyte, lymphocyte and monocyte counts obtained from a conventional automatic counter and by flow cytometry were compared. Results did not reveal statistically significant changes between the two techniques. O linfoma é o tumor de tecido hematopoético mais comum nos cães e um dos tumores malignos de maior ocorrência nesta espécie. É um ótimo modelo experimental para estudo devido a sua semelhança com o linfoma não-Hodgkin em humanos. Considerando a importância das alterações decorrentes da evolução desta neoplasia e aquelas ocorridas com o emprego da poliquimioterapia, avaliou-se o leucograma absoluto e a contagem de células CD45+ pela citometria de fluxo, no sangue de 25 cães com linfoma. Foram avaliados no momento do diagnóstico, uma vez por semana, durante as primeiras oito sessões quimioterápicas do protocolo de Madison-Wisconsin, e cujas contagens obtidas em contador automático convencional e por intermédio da citometria de fluxo, foram comparadas. Os resultados obtidos não revelaram diferenças estatisticamente significativas entre as duas técnicas utilizadas.UEL2013-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1330110.5433/1679-0359.2013v34n4p1793Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1793-1800Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1793-18001679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13301/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAnai, Letícia AbrahãoColeta, Flávia Eiras DelaMunhoz, Thiago DemarchiNogueira, Andressa Francisca SilvaSemolin, Lívia Maria SouzaVieira, Manuela CristinaSantana, Aureo Evangelista2023-01-16T16:44:40Zoai:ojs.pkp.sfu.ca:article/13301Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-16T16:44:40Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
Avaliação leucocitária de cães com linfoma submetidos ao protocolo de Madison-Wisconsin pela técnica convencional e citometria de fluxo
title Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
spellingShingle Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
Anai, Letícia Abrahão
CD45
Lymphoma
Dog.
CD45
Linfoma
Cão.
title_short Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
title_full Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
title_fullStr Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
title_full_unstemmed Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
title_sort Evaluation of leukocyte count in dogs with lymphoma submitted to the Madison-Wisconsin protocol by conventional technique and flow cytometry
author Anai, Letícia Abrahão
author_facet Anai, Letícia Abrahão
Coleta, Flávia Eiras Dela
Munhoz, Thiago Demarchi
Nogueira, Andressa Francisca Silva
Semolin, Lívia Maria Souza
Vieira, Manuela Cristina
Santana, Aureo Evangelista
author_role author
author2 Coleta, Flávia Eiras Dela
Munhoz, Thiago Demarchi
Nogueira, Andressa Francisca Silva
Semolin, Lívia Maria Souza
Vieira, Manuela Cristina
Santana, Aureo Evangelista
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Anai, Letícia Abrahão
Coleta, Flávia Eiras Dela
Munhoz, Thiago Demarchi
Nogueira, Andressa Francisca Silva
Semolin, Lívia Maria Souza
Vieira, Manuela Cristina
Santana, Aureo Evangelista
dc.subject.por.fl_str_mv CD45
Lymphoma
Dog.
CD45
Linfoma
Cão.
topic CD45
Lymphoma
Dog.
CD45
Linfoma
Cão.
description Lymphoma is the most common hematopoietic tumor in dogs and one of the malignant tumors with higher occurrence in this species. It is a great experimental model due to its resemblance with the non- Hodgkin lymphoma in humans. Considering the importance of the overall changes that result from to this kind of neoplastic tumor and those due to polichemotherapy this study aimed to evaluate the absolute leukon count and the total count of CD45+ cells in the blood of 25 dogs with lymphoma. Findings were crosschecked since diagnose and then once weekly during the first eight sessions of the Madison-Wisconsin chemotherapic protocol. Total granulocyte, lymphocyte and monocyte counts obtained from a conventional automatic counter and by flow cytometry were compared. Results did not reveal statistically significant changes between the two techniques. 
publishDate 2013
dc.date.none.fl_str_mv 2013-08-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13301
10.5433/1679-0359.2013v34n4p1793
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13301
identifier_str_mv 10.5433/1679-0359.2013v34n4p1793
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/13301/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2013 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2013 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1793-1800
Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1793-1800
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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