Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses

Detalhes bibliográficos
Autor(a) principal: Arneiro, Lídia Carolina Meira
Data de Publicação: 2012
Outros Autores: Curi, Rogério Adallah, Silva, Josineudson Augusto II Vasconcelos, Mota, Marcílio Dias Silveira da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516
Resumo: The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.
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spelling Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horsesCaracterização da variabilidade de genes relacionados à fertilidade de machos e ao temperamento em eqüinos da raça brasileira MangalargaCRISP1HorsesHTR1APolymorphismsSLC6A4.CavalosCRISP1HTR1APolimorfismosSLC6A4.Genética e Melhoramento dos Animais DomésticosThe aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.Os objetivos deste trabalho foram a padronização de metodologia de genotipagem por PCR-RFLP dos SNPs AJ_315378:c.110A>G e AB_264325:c.771G>C dos genes CRISP1 e HTR1A eqüino, respectivamente, bem como a caracterização em eqüinos da raça Mangalarga destes e de outro polimorfismo, o AB_098561:c.1470G>A do gene SLC6A4, a fim de promover o embasamento necessário para futuras pesquisas visando associação entre marcadores de DNA e características de interesse na raça. Para tanto, foram utilizados 151 animais Mangalarga, de ambos os sexos e de idades variadas, representativos da população do estado de São Paulo. O método de PCR-RFLP mostrou-se adequado para a genotipagem dos SNPs AJ315378:c.110A>G do CRISP1 e AB_264325:c.771G>C do HTR1A. Entretanto, o polimorfismo do CRISP1 provavelmente não ocorre em eqüinos Mangalarga, impossibilitando estudos de associação do marcador com características relacionadas à fertilidade de machos. As estimativas dos parâmetros genético-populacionais obtidos para os polimorfismos AB_264325:c.771G>C do HTR1A e o AB_098561:c.1470G>A do SLC6A4 na amostra de animais estudados desencorajam a realização de pesquisas visando a associação entre os marcadores e características relacionadas ao temperamento.UEL2012-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/951610.5433/1679-0359.2012v33n5p2001Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 2001-2010Semina: Ciências Agrárias; v. 33 n. 5 (2012); 2001-20101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516/11579Copyright (c) 2012 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessArneiro, Lídia Carolina MeiraCuri, Rogério AdallahSilva, Josineudson Augusto II VasconcelosMota, Marcílio Dias Silveira da2023-01-27T13:42:54Zoai:ojs.pkp.sfu.ca:article/9516Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-27T13:42:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
Caracterização da variabilidade de genes relacionados à fertilidade de machos e ao temperamento em eqüinos da raça brasileira Mangalarga
title Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
spellingShingle Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
Arneiro, Lídia Carolina Meira
CRISP1
Horses
HTR1A
Polymorphisms
SLC6A4.
Cavalos
CRISP1
HTR1A
Polimorfismos
SLC6A4.
Genética e Melhoramento dos Animais Domésticos
title_short Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
title_full Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
title_fullStr Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
title_full_unstemmed Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
title_sort Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
author Arneiro, Lídia Carolina Meira
author_facet Arneiro, Lídia Carolina Meira
Curi, Rogério Adallah
Silva, Josineudson Augusto II Vasconcelos
Mota, Marcílio Dias Silveira da
author_role author
author2 Curi, Rogério Adallah
Silva, Josineudson Augusto II Vasconcelos
Mota, Marcílio Dias Silveira da
author2_role author
author
author
dc.contributor.author.fl_str_mv Arneiro, Lídia Carolina Meira
Curi, Rogério Adallah
Silva, Josineudson Augusto II Vasconcelos
Mota, Marcílio Dias Silveira da
dc.subject.por.fl_str_mv CRISP1
Horses
HTR1A
Polymorphisms
SLC6A4.
Cavalos
CRISP1
HTR1A
Polimorfismos
SLC6A4.
Genética e Melhoramento dos Animais Domésticos
topic CRISP1
Horses
HTR1A
Polymorphisms
SLC6A4.
Cavalos
CRISP1
HTR1A
Polimorfismos
SLC6A4.
Genética e Melhoramento dos Animais Domésticos
description The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516
10.5433/1679-0359.2012v33n5p2001
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516
identifier_str_mv 10.5433/1679-0359.2012v33n5p2001
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516/11579
dc.rights.driver.fl_str_mv Copyright (c) 2012 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 2001-2010
Semina: Ciências Agrárias; v. 33 n. 5 (2012); 2001-2010
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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