Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516 |
Resumo: | The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament. |
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Semina. Ciências Agrárias (Online) |
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Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horsesCaracterização da variabilidade de genes relacionados à fertilidade de machos e ao temperamento em eqüinos da raça brasileira MangalargaCRISP1HorsesHTR1APolymorphismsSLC6A4.CavalosCRISP1HTR1APolimorfismosSLC6A4.Genética e Melhoramento dos Animais DomésticosThe aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.Os objetivos deste trabalho foram a padronização de metodologia de genotipagem por PCR-RFLP dos SNPs AJ_315378:c.110A>G e AB_264325:c.771G>C dos genes CRISP1 e HTR1A eqüino, respectivamente, bem como a caracterização em eqüinos da raça Mangalarga destes e de outro polimorfismo, o AB_098561:c.1470G>A do gene SLC6A4, a fim de promover o embasamento necessário para futuras pesquisas visando associação entre marcadores de DNA e características de interesse na raça. Para tanto, foram utilizados 151 animais Mangalarga, de ambos os sexos e de idades variadas, representativos da população do estado de São Paulo. O método de PCR-RFLP mostrou-se adequado para a genotipagem dos SNPs AJ315378:c.110A>G do CRISP1 e AB_264325:c.771G>C do HTR1A. Entretanto, o polimorfismo do CRISP1 provavelmente não ocorre em eqüinos Mangalarga, impossibilitando estudos de associação do marcador com características relacionadas à fertilidade de machos. As estimativas dos parâmetros genético-populacionais obtidos para os polimorfismos AB_264325:c.771G>C do HTR1A e o AB_098561:c.1470G>A do SLC6A4 na amostra de animais estudados desencorajam a realização de pesquisas visando a associação entre os marcadores e características relacionadas ao temperamento.UEL2012-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/951610.5433/1679-0359.2012v33n5p2001Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 2001-2010Semina: Ciências Agrárias; v. 33 n. 5 (2012); 2001-20101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516/11579Copyright (c) 2012 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessArneiro, Lídia Carolina MeiraCuri, Rogério AdallahSilva, Josineudson Augusto II VasconcelosMota, Marcílio Dias Silveira da2023-01-27T13:42:54Zoai:ojs.pkp.sfu.ca:article/9516Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-27T13:42:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses Caracterização da variabilidade de genes relacionados à fertilidade de machos e ao temperamento em eqüinos da raça brasileira Mangalarga |
title |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
spellingShingle |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses Arneiro, Lídia Carolina Meira CRISP1 Horses HTR1A Polymorphisms SLC6A4. Cavalos CRISP1 HTR1A Polimorfismos SLC6A4. Genética e Melhoramento dos Animais Domésticos |
title_short |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
title_full |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
title_fullStr |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
title_full_unstemmed |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
title_sort |
Characterization of the variability of genes related to male fertility and temperament in Brazilian Mangalarga horses |
author |
Arneiro, Lídia Carolina Meira |
author_facet |
Arneiro, Lídia Carolina Meira Curi, Rogério Adallah Silva, Josineudson Augusto II Vasconcelos Mota, Marcílio Dias Silveira da |
author_role |
author |
author2 |
Curi, Rogério Adallah Silva, Josineudson Augusto II Vasconcelos Mota, Marcílio Dias Silveira da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Arneiro, Lídia Carolina Meira Curi, Rogério Adallah Silva, Josineudson Augusto II Vasconcelos Mota, Marcílio Dias Silveira da |
dc.subject.por.fl_str_mv |
CRISP1 Horses HTR1A Polymorphisms SLC6A4. Cavalos CRISP1 HTR1A Polimorfismos SLC6A4. Genética e Melhoramento dos Animais Domésticos |
topic |
CRISP1 Horses HTR1A Polymorphisms SLC6A4. Cavalos CRISP1 HTR1A Polimorfismos SLC6A4. Genética e Melhoramento dos Animais Domésticos |
description |
The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ_315378:c.110A>G and AB_264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB_098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ_315378:c.110A>G of the CRISP1 and AB_264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB_264325:c.771G>C of the HTR1A and AB_098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516 10.5433/1679-0359.2012v33n5p2001 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516 |
identifier_str_mv |
10.5433/1679-0359.2012v33n5p2001 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/9516/11579 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 2001-2010 Semina: Ciências Agrárias; v. 33 n. 5 (2012); 2001-2010 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306065459806208 |