Transferability of heterologous microsatellite primers in Leiarius marmoratus

Detalhes bibliográficos
Autor(a) principal: Pandolfi, Victor César Freitas
Data de Publicação: 2020
Outros Autores: Yamachita, Andrei Lincoln, Souza, Felipe Pinheiro de, Lima, Ed Christian Suzuki de, Ayres, Denise Rocha, Bignardi, Annaiza Braga, Povh, Jayme Aparecido, Pereira, Ulisses de Pádua, Urrea-Rojas, Angela Maria, Lopera-Barrero, Nelson Mauricio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246
Resumo: The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies.
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spelling Transferability of heterologous microsatellite primers in Leiarius marmoratusTransferibilidade de primers microssatélites heterólogos em Leiarius marmoratusAquacultureAmazonian JundiáCross-amplificationGenetic conservationSSR.AquiculturaAmplificação cruzadaConservação genéticaJundiá amazônicoSSR.The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies.O Jundiá da Amazônia (Leiarius marmoratus) (Siluliformes: Pimelodidae) é uma espécie de catfish com importância social e econômica em alguns países Sul americanos como Brasil e Colômbia. A avaliação genética dessa espécie é limitada devido a ausência de marcadores moleculares específicos para L. marmoratus, dificultando estudos sobre a diversidade e estrutura genética em animais em condições de cativeiro ou em populações naturais. O objetivo do presente estudo foi avaliar a transferibilidade de 32 marcadores microssatélites heterólogos em Leiarius marmoratus. Os primers que apresentaram melhores padrões foram aplicados em 20 espécimes, sendo calculados o número de alelos (Na), número de alelos efetivos (Ne), diversidade gênica por Locus (GdL) e porcentagem de falha na amplificação (Md). Onze primers demonstraram padrões satisfatórios de transferibilidade, todos oriundos de peixes da família Pimelodidae, dos quais sete foram monomórficos e quatro polimórficos. Os onze marcadores apresentaram baixa porcentagem de Md (média 5,9% amostras por loco). O Na variou de um a dois alelos por loco, revelando baixo polimorfismo nas amostras avaliadas. O número médio de Ne e GdL foram 1,77 e 0,32, respectivamente. Estudos genéticos envolvendo indivíduos de diferentes regiões poderão apresentar novos alelos e maior número de loci polimórficos. A transferibilidade de loci microssatélites heterólogos em L. marmoratus se mostrou possível, no entanto, novos testes são necessários para aplicação desses marcadores em estudos genéticos populacionais.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3824610.5433/1679-0359.2020v41n5supl1p2297Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2297-2306Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2297-23061679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246/27806Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPandolfi, Victor César FreitasYamachita, Andrei LincolnSouza, Felipe Pinheiro deLima, Ed Christian Suzuki deAyres, Denise RochaBignardi, Annaiza BragaPovh, Jayme AparecidoPereira, Ulisses de PáduaUrrea-Rojas, Angela MariaLopera-Barrero, Nelson Mauricio2022-10-07T13:59:43Zoai:ojs.pkp.sfu.ca:article/38246Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T13:59:43Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Transferability of heterologous microsatellite primers in Leiarius marmoratus
Transferibilidade de primers microssatélites heterólogos em Leiarius marmoratus
title Transferability of heterologous microsatellite primers in Leiarius marmoratus
spellingShingle Transferability of heterologous microsatellite primers in Leiarius marmoratus
Pandolfi, Victor César Freitas
Aquaculture
Amazonian Jundiá
Cross-amplification
Genetic conservation
SSR.
Aquicultura
Amplificação cruzada
Conservação genética
Jundiá amazônico
SSR.
title_short Transferability of heterologous microsatellite primers in Leiarius marmoratus
title_full Transferability of heterologous microsatellite primers in Leiarius marmoratus
title_fullStr Transferability of heterologous microsatellite primers in Leiarius marmoratus
title_full_unstemmed Transferability of heterologous microsatellite primers in Leiarius marmoratus
title_sort Transferability of heterologous microsatellite primers in Leiarius marmoratus
author Pandolfi, Victor César Freitas
author_facet Pandolfi, Victor César Freitas
Yamachita, Andrei Lincoln
Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Ayres, Denise Rocha
Bignardi, Annaiza Braga
Povh, Jayme Aparecido
Pereira, Ulisses de Pádua
Urrea-Rojas, Angela Maria
Lopera-Barrero, Nelson Mauricio
author_role author
author2 Yamachita, Andrei Lincoln
Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Ayres, Denise Rocha
Bignardi, Annaiza Braga
Povh, Jayme Aparecido
Pereira, Ulisses de Pádua
Urrea-Rojas, Angela Maria
Lopera-Barrero, Nelson Mauricio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pandolfi, Victor César Freitas
Yamachita, Andrei Lincoln
Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Ayres, Denise Rocha
Bignardi, Annaiza Braga
Povh, Jayme Aparecido
Pereira, Ulisses de Pádua
Urrea-Rojas, Angela Maria
Lopera-Barrero, Nelson Mauricio
dc.subject.por.fl_str_mv Aquaculture
Amazonian Jundiá
Cross-amplification
Genetic conservation
SSR.
Aquicultura
Amplificação cruzada
Conservação genética
Jundiá amazônico
SSR.
topic Aquaculture
Amazonian Jundiá
Cross-amplification
Genetic conservation
SSR.
Aquicultura
Amplificação cruzada
Conservação genética
Jundiá amazônico
SSR.
description The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246
10.5433/1679-0359.2020v41n5supl1p2297
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246
identifier_str_mv 10.5433/1679-0359.2020v41n5supl1p2297
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246/27806
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2297-2306
Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2297-2306
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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