Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil

Detalhes bibliográficos
Autor(a) principal: Cacossi, Tami
Data de Publicação: 2019
Outros Autores: Dantas-Queiroz, Marcos Vinicius [UNESP], Palma-Silva, Clarisse [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s40415-019-00560-z
http://hdl.handle.net/11449/198285
Resumo: The Espinhaço Range is a center of biodiversity and endemism located in Eastern Brazil, and our knowledge is still scarce for the genetic diversity, structure and phylogeography of species from these mountains. Vriesea oligantha (Baker) Mez is an endemic bromeliad distributed along the Espinhaço Range with naturally fragmented populations. Here, the transferability of 30 microsatellites loci previously developed for seven Bromeliaceae species was tested from three different subfamilies in eight populations of V. oligantha. The amplification of 24 loci was successfully accomplished and 20 of them were polymorphic. Ten highly polymorphic microsatellite loci were selected to be amplified and genotyped in two population of V. oligantha. The number of alleles per locus ranged from 1 to 11, the expected and observed heterozygosities ranged from 0 to 0.905 and from 0 to 0.750, respectively. Our results endorse the cross-amplification between deeply divergent lineages of Bromeliaceae and provide useful markers for further phylogeographic, population genetics and mating systems studies to better understand the evolutionary history of an endemic species of a naturally fragmented area.
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spelling Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, BrazilCross-amplificationMonocotsPhylogeographyPopulation geneticsRocky fieldsSSR markersThe Espinhaço Range is a center of biodiversity and endemism located in Eastern Brazil, and our knowledge is still scarce for the genetic diversity, structure and phylogeography of species from these mountains. Vriesea oligantha (Baker) Mez is an endemic bromeliad distributed along the Espinhaço Range with naturally fragmented populations. Here, the transferability of 30 microsatellites loci previously developed for seven Bromeliaceae species was tested from three different subfamilies in eight populations of V. oligantha. The amplification of 24 loci was successfully accomplished and 20 of them were polymorphic. Ten highly polymorphic microsatellite loci were selected to be amplified and genotyped in two population of V. oligantha. The number of alleles per locus ranged from 1 to 11, the expected and observed heterozygosities ranged from 0 to 0.905 and from 0 to 0.750, respectively. Our results endorse the cross-amplification between deeply divergent lineages of Bromeliaceae and provide useful markers for further phylogeographic, population genetics and mating systems studies to better understand the evolutionary history of an endemic species of a naturally fragmented area.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Biologia Vegetal Instituto de Biologia Universidade Estadual de Campinas (Unicamp)Departamento de Ecologia Programa de Pós-graduação em Biologia Vegetal Universidade Estadual Paulista (Unesp)Departamento de Ecologia Programa de Pós-graduação em Biologia Vegetal Universidade Estadual Paulista (Unesp)FAPESP: 2018/07596-0CNPq: 300819/2016-1Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Cacossi, TamiDantas-Queiroz, Marcos Vinicius [UNESP]Palma-Silva, Clarisse [UNESP]2020-12-12T01:08:38Z2020-12-12T01:08:38Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article727-733http://dx.doi.org/10.1007/s40415-019-00560-zRevista Brasileira de Botanica, v. 42, n. 4, p. 727-733, 2019.1806-99590100-8404http://hdl.handle.net/11449/19828510.1007/s40415-019-00560-z2-s2.0-85076549780Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Botanicainfo:eu-repo/semantics/openAccess2021-10-23T10:11:28Zoai:repositorio.unesp.br:11449/198285Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:37:57.067916Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
title Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
spellingShingle Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
Cacossi, Tami
Cross-amplification
Monocots
Phylogeography
Population genetics
Rocky fields
SSR markers
title_short Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
title_full Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
title_fullStr Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
title_full_unstemmed Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
title_sort Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaço Range, Brazil
author Cacossi, Tami
author_facet Cacossi, Tami
Dantas-Queiroz, Marcos Vinicius [UNESP]
Palma-Silva, Clarisse [UNESP]
author_role author
author2 Dantas-Queiroz, Marcos Vinicius [UNESP]
Palma-Silva, Clarisse [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cacossi, Tami
Dantas-Queiroz, Marcos Vinicius [UNESP]
Palma-Silva, Clarisse [UNESP]
dc.subject.por.fl_str_mv Cross-amplification
Monocots
Phylogeography
Population genetics
Rocky fields
SSR markers
topic Cross-amplification
Monocots
Phylogeography
Population genetics
Rocky fields
SSR markers
description The Espinhaço Range is a center of biodiversity and endemism located in Eastern Brazil, and our knowledge is still scarce for the genetic diversity, structure and phylogeography of species from these mountains. Vriesea oligantha (Baker) Mez is an endemic bromeliad distributed along the Espinhaço Range with naturally fragmented populations. Here, the transferability of 30 microsatellites loci previously developed for seven Bromeliaceae species was tested from three different subfamilies in eight populations of V. oligantha. The amplification of 24 loci was successfully accomplished and 20 of them were polymorphic. Ten highly polymorphic microsatellite loci were selected to be amplified and genotyped in two population of V. oligantha. The number of alleles per locus ranged from 1 to 11, the expected and observed heterozygosities ranged from 0 to 0.905 and from 0 to 0.750, respectively. Our results endorse the cross-amplification between deeply divergent lineages of Bromeliaceae and provide useful markers for further phylogeographic, population genetics and mating systems studies to better understand the evolutionary history of an endemic species of a naturally fragmented area.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2020-12-12T01:08:38Z
2020-12-12T01:08:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s40415-019-00560-z
Revista Brasileira de Botanica, v. 42, n. 4, p. 727-733, 2019.
1806-9959
0100-8404
http://hdl.handle.net/11449/198285
10.1007/s40415-019-00560-z
2-s2.0-85076549780
url http://dx.doi.org/10.1007/s40415-019-00560-z
http://hdl.handle.net/11449/198285
identifier_str_mv Revista Brasileira de Botanica, v. 42, n. 4, p. 727-733, 2019.
1806-9959
0100-8404
10.1007/s40415-019-00560-z
2-s2.0-85076549780
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Botanica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 727-733
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128542097014784

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