Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil

Detalhes bibliográficos
Autor(a) principal: Bautista, José Luis Rodrigues
Data de Publicação: 2022
Outros Autores: Cordeiro, Matheus Dias, Araújo, Izabela Mesquita, Moraes, Paula de Abreu, Valin, Jaqueline Rodrigues de Almeida, Coelho, Juliana de Almeida, Figueiroa, Tays, Ferreira, Gilliard do Nascimento, Fonseca, Adivaldo Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44492
Resumo: The tick species and tick-borne pathogens present in a group of questing ticks collected from forest fragments in a rural area in the municipality of Divino, Minas Gerais state, Brazil were evaluated. The collected ticks were divided into two groups those collected from around the edges of the fragments and those collected from the interior of the forest. In all the fragments, the ticks were collected using a dragging and flagging technique and by harvesting them from white fabric gaiters. The larvae, nymphs, and adults were all morphologically identified using specific taxonomic keys. The larvae were identified to the genus level. DNA was extracted from the ticks and tested for the presence of Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. using a conventional polymerase chain reaction (PCR). In total, 1,122 questing ticks (750 larvae, 367 nymphs, and five adults) and 18 larval clusters were evaluated. The main species found in the collected tick population were Amblyomma sculptum, A. auricularium, A. aureolatum, and A. pseudoconcolor, along with the larvae of Amblyomma spp. and Dermacentor spp. None of the tick samples gave a positive result when tested by PCR for the presence of DNA from Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., or Theileria spp.
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spelling Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, BrazilCarrapatos na fase de vida livre em fragmentos de Mata Atlântica do município de Divino, Minas Gerais, BrasilArthropodsAmblyommahemoparasitesAtlantic rainforesttick-borne pathogens.ArtrópodesAmblyomma. HemoparasitosMata AtlânticaPatógenos associados a carrapatos.The tick species and tick-borne pathogens present in a group of questing ticks collected from forest fragments in a rural area in the municipality of Divino, Minas Gerais state, Brazil were evaluated. The collected ticks were divided into two groups those collected from around the edges of the fragments and those collected from the interior of the forest. In all the fragments, the ticks were collected using a dragging and flagging technique and by harvesting them from white fabric gaiters. The larvae, nymphs, and adults were all morphologically identified using specific taxonomic keys. The larvae were identified to the genus level. DNA was extracted from the ticks and tested for the presence of Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. using a conventional polymerase chain reaction (PCR). In total, 1,122 questing ticks (750 larvae, 367 nymphs, and five adults) and 18 larval clusters were evaluated. The main species found in the collected tick population were Amblyomma sculptum, A. auricularium, A. aureolatum, and A. pseudoconcolor, along with the larvae of Amblyomma spp. and Dermacentor spp. None of the tick samples gave a positive result when tested by PCR for the presence of DNA from Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., or Theileria spp.Neste estudo avaliou-se a presença de espécies de carrapatos e a detecção de agentes patogênicos a eles associados. Os carrapatos de vida livre coletados em fragmentos florestais da área rural do Município de Divino, Minas Gerais, Brasil. Os carrapatos coletados foram divididos em dois grupos, aqueles que foram coletados na área da margem do fragmento (Borda) e os coletados no interior da floresta (Mata). Em todos os fragmentos, os carrapatos foram coletados de acordo com a técnica de arraste aéreo ou no chão e com o uso de perneiras e de flanela. Tanto, as larvas, quanto as ninfas e os adultos foram morfologicamente identificados usando chaves taxonômicas específicas. No caso das larvas, estas foram identificadas até o nível de gênero. Foi realizada extração de DNA dos carrapatos e o DNA extraído foi testado para a presença de Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., e Theileria spp. por meio de uma estratégia de reação em cadeia da polimerase convencional. No total, 1.122 carrapatos em fase de vida livre (750 larvas, 367 ninfas e 5 adultos) e 18 clusters de larvas foram usados no estudo. As principais espécies identificadas na população de carrapatos coletada foram: Amblyomma sculptum, Amblyomma auricularium, Amblyomma aureolatum e Amblyomma pseudoconcolor e larvas de Amblyomma spp. e Dermacentor spp. Como resultado da detecção de patógenos não foi possível achar DNA de nenhum dos agentes analisados, assim todas as amostras de DNA dos carrapatos testados foram negativas tanto para Rickettsia spp. quanto para Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp. e Theileria spp.UEL2022-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4449210.5433/1679-0359.2022v43n3p1343Semina: Ciências Agrárias; Vol. 43 No. 3 (2022); 1343-1354Semina: Ciências Agrárias; v. 43 n. 3 (2022); 1343-13541679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44492/31651Copyright (c) 2022 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBautista, José Luis RodriguesCordeiro, Matheus DiasAraújo, Izabela MesquitaMoraes, Paula de AbreuValin, Jaqueline Rodrigues de AlmeidaCoelho, Juliana de AlmeidaFigueiroa, TaysFerreira, Gilliard do NascimentoFonseca, Adivaldo Henrique2022-09-19T16:25:16Zoai:ojs.pkp.sfu.ca:article/44492Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-19T16:25:16Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
Carrapatos na fase de vida livre em fragmentos de Mata Atlântica do município de Divino, Minas Gerais, Brasil
title Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
spellingShingle Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
Bautista, José Luis Rodrigues
Arthropods
Amblyomma
hemoparasites
Atlantic rainforest
tick-borne pathogens.
Artrópodes
Amblyomma. Hemoparasitos
Mata Atlântica
Patógenos associados a carrapatos.
title_short Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
title_full Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
title_fullStr Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
title_full_unstemmed Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
title_sort Questing ticks in Atlantic rainforest (Mata Atlantica) fragments in the municipality of Divino, Minas Gerais state, Brazil
author Bautista, José Luis Rodrigues
author_facet Bautista, José Luis Rodrigues
Cordeiro, Matheus Dias
Araújo, Izabela Mesquita
Moraes, Paula de Abreu
Valin, Jaqueline Rodrigues de Almeida
Coelho, Juliana de Almeida
Figueiroa, Tays
Ferreira, Gilliard do Nascimento
Fonseca, Adivaldo Henrique
author_role author
author2 Cordeiro, Matheus Dias
Araújo, Izabela Mesquita
Moraes, Paula de Abreu
Valin, Jaqueline Rodrigues de Almeida
Coelho, Juliana de Almeida
Figueiroa, Tays
Ferreira, Gilliard do Nascimento
Fonseca, Adivaldo Henrique
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bautista, José Luis Rodrigues
Cordeiro, Matheus Dias
Araújo, Izabela Mesquita
Moraes, Paula de Abreu
Valin, Jaqueline Rodrigues de Almeida
Coelho, Juliana de Almeida
Figueiroa, Tays
Ferreira, Gilliard do Nascimento
Fonseca, Adivaldo Henrique
dc.subject.por.fl_str_mv Arthropods
Amblyomma
hemoparasites
Atlantic rainforest
tick-borne pathogens.
Artrópodes
Amblyomma. Hemoparasitos
Mata Atlântica
Patógenos associados a carrapatos.
topic Arthropods
Amblyomma
hemoparasites
Atlantic rainforest
tick-borne pathogens.
Artrópodes
Amblyomma. Hemoparasitos
Mata Atlântica
Patógenos associados a carrapatos.
description The tick species and tick-borne pathogens present in a group of questing ticks collected from forest fragments in a rural area in the municipality of Divino, Minas Gerais state, Brazil were evaluated. The collected ticks were divided into two groups those collected from around the edges of the fragments and those collected from the interior of the forest. In all the fragments, the ticks were collected using a dragging and flagging technique and by harvesting them from white fabric gaiters. The larvae, nymphs, and adults were all morphologically identified using specific taxonomic keys. The larvae were identified to the genus level. DNA was extracted from the ticks and tested for the presence of Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. using a conventional polymerase chain reaction (PCR). In total, 1,122 questing ticks (750 larvae, 367 nymphs, and five adults) and 18 larval clusters were evaluated. The main species found in the collected tick population were Amblyomma sculptum, A. auricularium, A. aureolatum, and A. pseudoconcolor, along with the larvae of Amblyomma spp. and Dermacentor spp. None of the tick samples gave a positive result when tested by PCR for the presence of DNA from Rickettsia spp., Borrelia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., or Theileria spp.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44492
10.5433/1679-0359.2022v43n3p1343
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44492
identifier_str_mv 10.5433/1679-0359.2022v43n3p1343
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44492/31651
dc.rights.driver.fl_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 43 No. 3 (2022); 1343-1354
Semina: Ciências Agrárias; v. 43 n. 3 (2022); 1343-1354
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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