Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds

Detalhes bibliográficos
Autor(a) principal: Dranski, João Alexandre Lopes
Data de Publicação: 2017
Outros Autores: Malavasi, Marlene de Matos, Malavasi, Ubirajara Contro, Schuster, Ivan, Lazaretti, Norma Schlickmann
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484
Resumo: The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method.
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spelling Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seedsDióxido de carbono quantificado pelo infravermelho na avaliação da atividade respiratória em sementes de milhoZea mays LCO2Infrared spectroscopyValidation.Zea mays LCO2Espectroscopia no infravermelhoValidação.The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method.A atividade respiratória é uma das primeiras manifestações biológicas da perda de vigor e sua detecção pode auxiliar no monitoramento e no controle de qualidade da produção de sementes pelas indústrias sementeiras. Objetivou-se desenvolver e validar a metodologia da concentração de dióxido de carbono na avaliação da qualidade fisiológica de sementes Zea mays L. com base na espectroscopia no infravermelho. O método proposto quantifica o conteúdo de CO2 através de um sistema de arraste e exaustão do gás liberado pelas sementes. Amostras de 15, 20 e 25 sementes, de 6 lotes da cultivar CD 384 Hx foram incubadas sob temperaturas de 15, 25 e 40 oC. Quantificou-se o conteúdo de CO2 liberado após 1, 3 6. 9. 12, e 24 h de incubação. Paralelamente, avaliou-se a porcentagem de plântulas normais emergidas em campo. Foram calculados os coeficientes de correlação simples entre os testes. Após a determinação das condições de leitura, avaliou-se a exatidão e precisão utilizando 15 lotes de sementes. Para a avaliação da atividade respiratória em sementes de Z. mays recomenda-se uma amostra de 25 sementes, incubadas a 15 oC por um período máximo de 12 h, o que possibilita classificar lotes em diferentes níveis de vigor e predizer o estabelecimento de plântulas em campo, sendo o método adequado para a mensuração de CO2, pois externa precisão entre medidas sucessivas e concordância ao método de referência.UEL2017-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2448410.5433/1679-0359.2017v38n3p1115Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1115-1132Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1115-11321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484/20985Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessDranski, João Alexandre LopesMalavasi, Marlene de MatosMalavasi, Ubirajara ControSchuster, IvanLazaretti, Norma Schlickmann2022-10-24T13:10:18Zoai:ojs.pkp.sfu.ca:article/24484Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T13:10:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
Dióxido de carbono quantificado pelo infravermelho na avaliação da atividade respiratória em sementes de milho
title Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
spellingShingle Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
Dranski, João Alexandre Lopes
Zea mays L
CO2
Infrared spectroscopy
Validation.
Zea mays L
CO2
Espectroscopia no infravermelho
Validação.
title_short Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
title_full Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
title_fullStr Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
title_full_unstemmed Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
title_sort Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
author Dranski, João Alexandre Lopes
author_facet Dranski, João Alexandre Lopes
Malavasi, Marlene de Matos
Malavasi, Ubirajara Contro
Schuster, Ivan
Lazaretti, Norma Schlickmann
author_role author
author2 Malavasi, Marlene de Matos
Malavasi, Ubirajara Contro
Schuster, Ivan
Lazaretti, Norma Schlickmann
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dranski, João Alexandre Lopes
Malavasi, Marlene de Matos
Malavasi, Ubirajara Contro
Schuster, Ivan
Lazaretti, Norma Schlickmann
dc.subject.por.fl_str_mv Zea mays L
CO2
Infrared spectroscopy
Validation.
Zea mays L
CO2
Espectroscopia no infravermelho
Validação.
topic Zea mays L
CO2
Infrared spectroscopy
Validation.
Zea mays L
CO2
Espectroscopia no infravermelho
Validação.
description The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484
10.5433/1679-0359.2017v38n3p1115
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484
identifier_str_mv 10.5433/1679-0359.2017v38n3p1115
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484/20985
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1115-1132
Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1115-1132
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306075294400512

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