Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484 |
Resumo: | The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method. |
id |
UEL-11_30ead3f7820065fa47ec1530b0ff1c48 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/24484 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seedsDióxido de carbono quantificado pelo infravermelho na avaliação da atividade respiratória em sementes de milhoZea mays LCO2Infrared spectroscopyValidation.Zea mays LCO2Espectroscopia no infravermelhoValidação.The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method.A atividade respiratória é uma das primeiras manifestações biológicas da perda de vigor e sua detecção pode auxiliar no monitoramento e no controle de qualidade da produção de sementes pelas indústrias sementeiras. Objetivou-se desenvolver e validar a metodologia da concentração de dióxido de carbono na avaliação da qualidade fisiológica de sementes Zea mays L. com base na espectroscopia no infravermelho. O método proposto quantifica o conteúdo de CO2 através de um sistema de arraste e exaustão do gás liberado pelas sementes. Amostras de 15, 20 e 25 sementes, de 6 lotes da cultivar CD 384 Hx foram incubadas sob temperaturas de 15, 25 e 40 oC. Quantificou-se o conteúdo de CO2 liberado após 1, 3 6. 9. 12, e 24 h de incubação. Paralelamente, avaliou-se a porcentagem de plântulas normais emergidas em campo. Foram calculados os coeficientes de correlação simples entre os testes. Após a determinação das condições de leitura, avaliou-se a exatidão e precisão utilizando 15 lotes de sementes. Para a avaliação da atividade respiratória em sementes de Z. mays recomenda-se uma amostra de 25 sementes, incubadas a 15 oC por um período máximo de 12 h, o que possibilita classificar lotes em diferentes níveis de vigor e predizer o estabelecimento de plântulas em campo, sendo o método adequado para a mensuração de CO2, pois externa precisão entre medidas sucessivas e concordância ao método de referência.UEL2017-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2448410.5433/1679-0359.2017v38n3p1115Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1115-1132Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1115-11321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484/20985Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessDranski, João Alexandre LopesMalavasi, Marlene de MatosMalavasi, Ubirajara ControSchuster, IvanLazaretti, Norma Schlickmann2022-10-24T13:10:18Zoai:ojs.pkp.sfu.ca:article/24484Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T13:10:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds Dióxido de carbono quantificado pelo infravermelho na avaliação da atividade respiratória em sementes de milho |
title |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
spellingShingle |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds Dranski, João Alexandre Lopes Zea mays L CO2 Infrared spectroscopy Validation. Zea mays L CO2 Espectroscopia no infravermelho Validação. |
title_short |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
title_full |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
title_fullStr |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
title_full_unstemmed |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
title_sort |
Carbon dioxide quantified by the infrared in respiratory activity evaluation in corn seeds |
author |
Dranski, João Alexandre Lopes |
author_facet |
Dranski, João Alexandre Lopes Malavasi, Marlene de Matos Malavasi, Ubirajara Contro Schuster, Ivan Lazaretti, Norma Schlickmann |
author_role |
author |
author2 |
Malavasi, Marlene de Matos Malavasi, Ubirajara Contro Schuster, Ivan Lazaretti, Norma Schlickmann |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Dranski, João Alexandre Lopes Malavasi, Marlene de Matos Malavasi, Ubirajara Contro Schuster, Ivan Lazaretti, Norma Schlickmann |
dc.subject.por.fl_str_mv |
Zea mays L CO2 Infrared spectroscopy Validation. Zea mays L CO2 Espectroscopia no infravermelho Validação. |
topic |
Zea mays L CO2 Infrared spectroscopy Validation. Zea mays L CO2 Espectroscopia no infravermelho Validação. |
description |
The respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of the respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484 10.5433/1679-0359.2017v38n3p1115 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484 |
identifier_str_mv |
10.5433/1679-0359.2017v38n3p1115 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24484/20985 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1115-1132 Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1115-1132 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306075294400512 |