Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities

Detalhes bibliográficos
Autor(a) principal: Agostini, Juliana da Silva
Data de Publicação: 2006
Outros Autores: Ida, Elza Iouko
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2398
Resumo: The response surface methodology was applied to investigate both length of time and temperature of the sunflower germination process on the phytate reduction and to enhancig phytase and acid phosphatase activities. The hybrid (Helianthus annuus) M734 was used and the factorial design 22 with two repetitions at the central point working with two variables time and temperature at two levels, completing four experiments with two repetitions at central point were applied. Experiments were performed randomly and the germination process was carried out using a germination camera at 25ºC under fluorescent illumination during 8 days. Seeds were collected from the second day of experiment, lyophilized and ground in order to process the quantitative analysis of phytate and the enzymes activity. Results showed that the length of time and temperature were significant on the reduction of phytate amount and the occurrence of the maximum enzymes activity. The maximum phytate reduction and the acid phosphatase activity was observed at the sixth day of seed germination at 30ºC while for phytase activity the best conditions were at the fourth day at the same temperature.
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spelling Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activitiesEfeito das condições de germinação de girassol na redução do teor de fitato e ativação de fitase e fosfatase ácidaSunflower germinationPhytatePhytaseAcid phosphataseResponse surface methodology.Germinação de girassolFitatoFitaseFosafatase ácidaMetodologia de superfície de resposta.The response surface methodology was applied to investigate both length of time and temperature of the sunflower germination process on the phytate reduction and to enhancig phytase and acid phosphatase activities. The hybrid (Helianthus annuus) M734 was used and the factorial design 22 with two repetitions at the central point working with two variables time and temperature at two levels, completing four experiments with two repetitions at central point were applied. Experiments were performed randomly and the germination process was carried out using a germination camera at 25ºC under fluorescent illumination during 8 days. Seeds were collected from the second day of experiment, lyophilized and ground in order to process the quantitative analysis of phytate and the enzymes activity. Results showed that the length of time and temperature were significant on the reduction of phytate amount and the occurrence of the maximum enzymes activity. The maximum phytate reduction and the acid phosphatase activity was observed at the sixth day of seed germination at 30ºC while for phytase activity the best conditions were at the fourth day at the same temperature.A metodologia de superfície de resposta foi aplicada para investigar o efeito do tempo e temperatura do processo de germinação na redução do teor de fitato e na ativação das enzimas fitase e fosfatase ácida de girassol híbrido(Helianthus annuus) M734. Foi utilizado o delineamento fatorial 22 com duas repetições no ponto central com as variáveis tempo e temperatura em dois níveis, totalizando quatro experimentos com duas repetições no ponto central. Os experimentos foram conduzidos aleatoriamente e o procedimento de germinação foi realizado em câmara de germinação regulada a 25°C com iluminação fluorescente externa por oito dias. As sementes foram coletadas a partir do segundo dia, liofilizadas e moídas para análise de fitato e atividades de fitase e fosfatase ácida. Foi observado que o tempo e temperatura foram significativos na redução do teor de fitato e ocorrência de máxima atividades de fitase e fosfatase ácida na germinação do girassol híbrido. A maior redução do teor de fitato e máxima atividade de fosfatase ácida do girassol híbrido ocorreu no sexto dia de germinação a 30ºC. Entretanto, a máxima atividade de fitase ocorreu no quarto dia de germinação a 30ºC.UEL2006-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/239810.5433/1679-0359.2006v27n1p61Semina: Ciências Agrárias; Vol. 27 No. 1 (2006); 61-70Semina: Ciências Agrárias; v. 27 n. 1 (2006); 61-701679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2398/2053Agostini, Juliana da SilvaIda, Elza Ioukoinfo:eu-repo/semantics/openAccess2010-03-15T16:54:06Zoai:ojs.pkp.sfu.ca:article/2398Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-15T16:54:06Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
Efeito das condições de germinação de girassol na redução do teor de fitato e ativação de fitase e fosfatase ácida
title Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
spellingShingle Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
Agostini, Juliana da Silva
Sunflower germination
Phytate
Phytase
Acid phosphatase
Response surface methodology.
Germinação de girassol
Fitato
Fitase
Fosafatase ácida
Metodologia de superfície de resposta.
title_short Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
title_full Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
title_fullStr Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
title_full_unstemmed Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
title_sort Effect of sunflower germination conditions for reducing phytate content and to enhancing phytase and acid phospatase activities
author Agostini, Juliana da Silva
author_facet Agostini, Juliana da Silva
Ida, Elza Iouko
author_role author
author2 Ida, Elza Iouko
author2_role author
dc.contributor.author.fl_str_mv Agostini, Juliana da Silva
Ida, Elza Iouko
dc.subject.por.fl_str_mv Sunflower germination
Phytate
Phytase
Acid phosphatase
Response surface methodology.
Germinação de girassol
Fitato
Fitase
Fosafatase ácida
Metodologia de superfície de resposta.
topic Sunflower germination
Phytate
Phytase
Acid phosphatase
Response surface methodology.
Germinação de girassol
Fitato
Fitase
Fosafatase ácida
Metodologia de superfície de resposta.
description The response surface methodology was applied to investigate both length of time and temperature of the sunflower germination process on the phytate reduction and to enhancig phytase and acid phosphatase activities. The hybrid (Helianthus annuus) M734 was used and the factorial design 22 with two repetitions at the central point working with two variables time and temperature at two levels, completing four experiments with two repetitions at central point were applied. Experiments were performed randomly and the germination process was carried out using a germination camera at 25ºC under fluorescent illumination during 8 days. Seeds were collected from the second day of experiment, lyophilized and ground in order to process the quantitative analysis of phytate and the enzymes activity. Results showed that the length of time and temperature were significant on the reduction of phytate amount and the occurrence of the maximum enzymes activity. The maximum phytate reduction and the acid phosphatase activity was observed at the sixth day of seed germination at 30ºC while for phytase activity the best conditions were at the fourth day at the same temperature.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2398
10.5433/1679-0359.2006v27n1p61
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2398
identifier_str_mv 10.5433/1679-0359.2006v27n1p61
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2398/2053
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 27 No. 1 (2006); 61-70
Semina: Ciências Agrárias; v. 27 n. 1 (2006); 61-70
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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