Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Pacheco, Filipe Corrêa
Data de Publicação: 2017
Outros Autores: Moraes Filho, Jonas, Rocha, Vlamir José, Sampieri, Bruno Rodrigues, Zaniolo, Melissa Marchi, Pachaly, José Ricardo, Otutumi, Luciana Kazue, Vidotto, Odilon, Labruna, Marcelo Bahia, Gonçalves, Daniela Dib
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290
Resumo: Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis.
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spelling Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, BrazilAnticorpos anti-Rickettsia rickettsii em capivaras (Hydrochoerus hydrochaeris Linnaeus, 1766) de uma de uma região agrícola de Araras, São Paulo, BrasilTicksSerological diagnosisSpotted FeverHumanZoonosis.CarrapatosDiagnóstico sorológicoFebre maculosaHumanoZoonose.Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis.A febre maculosa é uma doença infecciosa, causada por rickéttsias do Grupo da Febre Maculosa, que geralmente se desenvolve em caráter endêmico e tem como principal agente etiológico Rickettsia rickettsii. Apresenta elevadas taxas de letalidade no Brasil, e a transmissão do agente ao homem ou animal ocorre pela picada de carrapatos infectados. A capivara (Hydrochoerus hydrochaeris) é um importante reservatório de Rickettsia spp., e por meio de infecção experimental demonstrou-se sua capacidade de mantê-la circulante no organismo, sem apresentar sinais clínicos da doença. Considerando o elevado potencial zoonótico e os prejuízos causados na saúde única por esse agente, este trabalho teve o objetivo de detectar anticorpos anti-Rickettsia rickettsii em capivara (Hydrochoerus hydrochaeris) de um bosque urbano de Araras, São Paulo, Brasil. Foi utilizada a técnica de imunofluorescência indireta (IFA) para detectar anticorpos anti-R. rickettsii. Das 28 amostras de soro testadas na IFA, 18 (64,28%) foram consideradas reagentes com títulos de anticorpos variando de 256 a 2048, sendo que sete (38,88%) amostras apresentaram títulos de 256, três (16,67%) títulos de 512, cinco (27,78%) títulos de 1024 e três (16,67%) títulos de 2048 e não foi possível a associação da variável sexo (p?0.05) com os resultados sorológicos para Rickettsia rickettsii. Outros estudos serão necessários para esclarecer que títulos sorológicos na IFA podem assegurar a positividade da infecção na capivara, a partir de avaliação clínica e laboratorial frente à rickettsemia, e estabelecer a relação entre títulos e a cronicidade da doença. Isso decorre da possibilidade de ocorrência de reações cruzadas com outras espécies de rickéttsias dos mesmos subgrupos, levando à necessidade da realização de testes moleculares para se confirmar o diagnóstico para a enfermidade.UEL2017-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionSHORT COMMUNICATIONapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2729010.5433/1679-0359.2017v38n4Supl1p2543Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2543-2550Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2543-25501679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290/21503Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPacheco, Filipe CorrêaMoraes Filho, JonasRocha, Vlamir JoséSampieri, Bruno RodriguesZaniolo, Melissa MarchiPachaly, José RicardoOtutumi, Luciana KazueVidotto, OdilonLabruna, Marcelo BahiaGonçalves, Daniela Dib2022-10-21T15:46:53Zoai:ojs.pkp.sfu.ca:article/27290Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T15:46:53Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
Anticorpos anti-Rickettsia rickettsii em capivaras (Hydrochoerus hydrochaeris Linnaeus, 1766) de uma de uma região agrícola de Araras, São Paulo, Brasil
title Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
spellingShingle Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
Pacheco, Filipe Corrêa
Ticks
Serological diagnosis
Spotted Fever
Human
Zoonosis.
Carrapatos
Diagnóstico sorológico
Febre maculosa
Humano
Zoonose.
title_short Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
title_full Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
title_fullStr Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
title_full_unstemmed Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
title_sort Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
author Pacheco, Filipe Corrêa
author_facet Pacheco, Filipe Corrêa
Moraes Filho, Jonas
Rocha, Vlamir José
Sampieri, Bruno Rodrigues
Zaniolo, Melissa Marchi
Pachaly, José Ricardo
Otutumi, Luciana Kazue
Vidotto, Odilon
Labruna, Marcelo Bahia
Gonçalves, Daniela Dib
author_role author
author2 Moraes Filho, Jonas
Rocha, Vlamir José
Sampieri, Bruno Rodrigues
Zaniolo, Melissa Marchi
Pachaly, José Ricardo
Otutumi, Luciana Kazue
Vidotto, Odilon
Labruna, Marcelo Bahia
Gonçalves, Daniela Dib
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pacheco, Filipe Corrêa
Moraes Filho, Jonas
Rocha, Vlamir José
Sampieri, Bruno Rodrigues
Zaniolo, Melissa Marchi
Pachaly, José Ricardo
Otutumi, Luciana Kazue
Vidotto, Odilon
Labruna, Marcelo Bahia
Gonçalves, Daniela Dib
dc.subject.por.fl_str_mv Ticks
Serological diagnosis
Spotted Fever
Human
Zoonosis.
Carrapatos
Diagnóstico sorológico
Febre maculosa
Humano
Zoonose.
topic Ticks
Serological diagnosis
Spotted Fever
Human
Zoonosis.
Carrapatos
Diagnóstico sorológico
Febre maculosa
Humano
Zoonose.
description Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-25
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url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290
identifier_str_mv 10.5433/1679-0359.2017v38n4Supl1p2543
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290/21503
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2543-2550
Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2543-2550
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
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instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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