Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290 |
Resumo: | Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis. |
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Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, BrazilAnticorpos anti-Rickettsia rickettsii em capivaras (Hydrochoerus hydrochaeris Linnaeus, 1766) de uma de uma região agrícola de Araras, São Paulo, BrasilTicksSerological diagnosisSpotted FeverHumanZoonosis.CarrapatosDiagnóstico sorológicoFebre maculosaHumanoZoonose.Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis.A febre maculosa é uma doença infecciosa, causada por rickéttsias do Grupo da Febre Maculosa, que geralmente se desenvolve em caráter endêmico e tem como principal agente etiológico Rickettsia rickettsii. Apresenta elevadas taxas de letalidade no Brasil, e a transmissão do agente ao homem ou animal ocorre pela picada de carrapatos infectados. A capivara (Hydrochoerus hydrochaeris) é um importante reservatório de Rickettsia spp., e por meio de infecção experimental demonstrou-se sua capacidade de mantê-la circulante no organismo, sem apresentar sinais clínicos da doença. Considerando o elevado potencial zoonótico e os prejuízos causados na saúde única por esse agente, este trabalho teve o objetivo de detectar anticorpos anti-Rickettsia rickettsii em capivara (Hydrochoerus hydrochaeris) de um bosque urbano de Araras, São Paulo, Brasil. Foi utilizada a técnica de imunofluorescência indireta (IFA) para detectar anticorpos anti-R. rickettsii. Das 28 amostras de soro testadas na IFA, 18 (64,28%) foram consideradas reagentes com títulos de anticorpos variando de 256 a 2048, sendo que sete (38,88%) amostras apresentaram títulos de 256, três (16,67%) títulos de 512, cinco (27,78%) títulos de 1024 e três (16,67%) títulos de 2048 e não foi possível a associação da variável sexo (p?0.05) com os resultados sorológicos para Rickettsia rickettsii. Outros estudos serão necessários para esclarecer que títulos sorológicos na IFA podem assegurar a positividade da infecção na capivara, a partir de avaliação clínica e laboratorial frente à rickettsemia, e estabelecer a relação entre títulos e a cronicidade da doença. Isso decorre da possibilidade de ocorrência de reações cruzadas com outras espécies de rickéttsias dos mesmos subgrupos, levando à necessidade da realização de testes moleculares para se confirmar o diagnóstico para a enfermidade.UEL2017-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionSHORT COMMUNICATIONapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2729010.5433/1679-0359.2017v38n4Supl1p2543Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2543-2550Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2543-25501679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290/21503Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPacheco, Filipe CorrêaMoraes Filho, JonasRocha, Vlamir JoséSampieri, Bruno RodriguesZaniolo, Melissa MarchiPachaly, José RicardoOtutumi, Luciana KazueVidotto, OdilonLabruna, Marcelo BahiaGonçalves, Daniela Dib2022-10-21T15:46:53Zoai:ojs.pkp.sfu.ca:article/27290Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T15:46:53Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil Anticorpos anti-Rickettsia rickettsii em capivaras (Hydrochoerus hydrochaeris Linnaeus, 1766) de uma de uma região agrícola de Araras, São Paulo, Brasil |
title |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
spellingShingle |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil Pacheco, Filipe Corrêa Ticks Serological diagnosis Spotted Fever Human Zoonosis. Carrapatos Diagnóstico sorológico Febre maculosa Humano Zoonose. |
title_short |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
title_full |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
title_fullStr |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
title_full_unstemmed |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
title_sort |
Anti-Rickettsia rickettsii antibodies in capybaras (Hydrochoerus hydrochaeris Linnaeus, 1766) from an agricultural landscape in Araras, São Paulo, Brazil |
author |
Pacheco, Filipe Corrêa |
author_facet |
Pacheco, Filipe Corrêa Moraes Filho, Jonas Rocha, Vlamir José Sampieri, Bruno Rodrigues Zaniolo, Melissa Marchi Pachaly, José Ricardo Otutumi, Luciana Kazue Vidotto, Odilon Labruna, Marcelo Bahia Gonçalves, Daniela Dib |
author_role |
author |
author2 |
Moraes Filho, Jonas Rocha, Vlamir José Sampieri, Bruno Rodrigues Zaniolo, Melissa Marchi Pachaly, José Ricardo Otutumi, Luciana Kazue Vidotto, Odilon Labruna, Marcelo Bahia Gonçalves, Daniela Dib |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pacheco, Filipe Corrêa Moraes Filho, Jonas Rocha, Vlamir José Sampieri, Bruno Rodrigues Zaniolo, Melissa Marchi Pachaly, José Ricardo Otutumi, Luciana Kazue Vidotto, Odilon Labruna, Marcelo Bahia Gonçalves, Daniela Dib |
dc.subject.por.fl_str_mv |
Ticks Serological diagnosis Spotted Fever Human Zoonosis. Carrapatos Diagnóstico sorológico Febre maculosa Humano Zoonose. |
topic |
Ticks Serological diagnosis Spotted Fever Human Zoonosis. Carrapatos Diagnóstico sorológico Febre maculosa Humano Zoonose. |
description |
Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion SHORT COMMUNICATION |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290 10.5433/1679-0359.2017v38n4Supl1p2543 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290 |
identifier_str_mv |
10.5433/1679-0359.2017v38n4Supl1p2543 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27290/21503 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2543-2550 Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2543-2550 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306055888404480 |