Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39339 |
Resumo: | The Amazonian fish Tambaqui (Colossoma macropomum) is the most common native species in Brazil. This species has the highest production rate in the Northern region, especially in the State of Rondônia. The genetic evaluation of Tambaqui is an extremely important to increase productivity in fish farms or improve the adaptability in restocking natural populations. The objective of this study was to evaluate the genetic diversity of three Tambaqui broodstocks in Rondônia, Brazil. Six microsatellite markers were used to analyze a total of 89 breeders collected from three fish farms located in Ji-Paraná (JP), Ouro Preto do Oeste (OP) and Presidente Médici (PM). A total of 37 alleles between 140 and 310 bp were found, including the presence of exclusive and low frequency alleles in the three broodstocks. The average values of observed heterozygosity ranged from 0.404 (PM) to 0.499 (JP). The FIS coefficient values were positive for the three broodstocks, demonstrating a deficit of heterozygotes. The Molecular Variance Analysis (AMOVA) showed greater variation within the stocks than between them. The genetic differentiation was moderate and significant between the stocks, with higher differentiation between JP x PM and lower between OP x PM. The Bayesian analysis designated an optimal value of K = 3 groupings. Although there is moderate genetic diversity between broodstocks, the high FIS indicates a possible decline of diversity in the next generations, and therefore, the incorporation of new breeders is suggested to increase the genetic diversity in the three stocks. |
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Semina. Ciências Agrárias (Online) |
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Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markersDiversidade genética de estoque de Tambaqui (Teleostei - Characidae) da região Norte do Brasil usando marcadores microssatélitesColossoma macropomumConservationGenetic variabilityMicrosatellitesSSR.Colossoma macropomumConservaçãoMicrossatélitesSSRVariabilidade genética.The Amazonian fish Tambaqui (Colossoma macropomum) is the most common native species in Brazil. This species has the highest production rate in the Northern region, especially in the State of Rondônia. The genetic evaluation of Tambaqui is an extremely important to increase productivity in fish farms or improve the adaptability in restocking natural populations. The objective of this study was to evaluate the genetic diversity of three Tambaqui broodstocks in Rondônia, Brazil. Six microsatellite markers were used to analyze a total of 89 breeders collected from three fish farms located in Ji-Paraná (JP), Ouro Preto do Oeste (OP) and Presidente Médici (PM). A total of 37 alleles between 140 and 310 bp were found, including the presence of exclusive and low frequency alleles in the three broodstocks. The average values of observed heterozygosity ranged from 0.404 (PM) to 0.499 (JP). The FIS coefficient values were positive for the three broodstocks, demonstrating a deficit of heterozygotes. The Molecular Variance Analysis (AMOVA) showed greater variation within the stocks than between them. The genetic differentiation was moderate and significant between the stocks, with higher differentiation between JP x PM and lower between OP x PM. The Bayesian analysis designated an optimal value of K = 3 groupings. Although there is moderate genetic diversity between broodstocks, the high FIS indicates a possible decline of diversity in the next generations, and therefore, the incorporation of new breeders is suggested to increase the genetic diversity in the three stocks.O peixe amazônico Tambaqui (Colossoma macropomum) é a espécie nativa mais produzida no Brasil. Esta espécie é a mais criada na região Norte, principalmente no Estado de Rondônia. A avaliação genética de Tambaqui é extremamente importante para aumentar a produtividade nas pisciculturas ou melhorar a adaptabilidade no repovoamento de populações naturais. O objetivo deste estudo foi avaliar a diversidade genética de três estoques de Tambaqui em Rondônia, Brasil. Seis marcadores microssatélites foram utilizados para analisar um total de 89 reprodutores coletados em três pisciculturas localizadas em Ji-Paraná (JP), Ouro Preto do Oeste (OP) e Presidente Médici (PM). Foram encontrados 37 alelos entre 140 e 310 pb, incluindo a presença de alelos exclusivos e de baixa frequência nas três ninhadas. Os valores médios de heterozigosidade observada variaram de 0,404 (PM) a 0,499 (JP). Os valores do coeficiente de FIS foram positivos para as três ninhadas, demonstrando déficit de heterozigotos. A Análise de Variância Molecular (AMOVA) mostrou maior variação dentro dos estoques do que entre eles. A diferenciação genética foi moderada e significativa entre os estoques, com maior diferenciação entre JP x PM e menor entre OP x PM. A análise bayesiana designou um valor ótimo de K = 3 agrupamentos. Embora exista uma diversidade genética moderada entre os filhotes, o alto SIF indica um possível declínio da diversidade nas próximas gerações e, portanto, sugere-se a incorporação de novos criadores para aumentar a diversidade genética nos três estoques.UEL2020-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa básicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3933910.5433/1679-0359.2020v41n6Supl2p3249Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3249-3258Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3249-32581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39339/28281Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessUrrea-Rojas, Angela MariaSouza, Felipe Pinheiro deLima, Ed Christian Suzuki deYamachita, Andrei LincolnPandolfi, Victor César FreitasGodoy, Sara Mataroli deRibeiro, Ricardo PereiraPovh, Jayme AparecidoPereira, Ulisses de PáduaLopera-Barrero, Nelson Mauricio2022-10-06T11:14:37Zoai:ojs.pkp.sfu.ca:article/39339Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-06T11:14:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers Diversidade genética de estoque de Tambaqui (Teleostei - Characidae) da região Norte do Brasil usando marcadores microssatélites |
title |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
spellingShingle |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers Urrea-Rojas, Angela Maria Colossoma macropomum Conservation Genetic variability Microsatellites SSR. Colossoma macropomum Conservação Microssatélites SSR Variabilidade genética. |
title_short |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
title_full |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
title_fullStr |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
title_full_unstemmed |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
title_sort |
Genetic diversity of Tambaqui (Teleostei - Characidae) broodstocks from Northern region of Brazil using microsatellite markers |
author |
Urrea-Rojas, Angela Maria |
author_facet |
Urrea-Rojas, Angela Maria Souza, Felipe Pinheiro de Lima, Ed Christian Suzuki de Yamachita, Andrei Lincoln Pandolfi, Victor César Freitas Godoy, Sara Mataroli de Ribeiro, Ricardo Pereira Povh, Jayme Aparecido Pereira, Ulisses de Pádua Lopera-Barrero, Nelson Mauricio |
author_role |
author |
author2 |
Souza, Felipe Pinheiro de Lima, Ed Christian Suzuki de Yamachita, Andrei Lincoln Pandolfi, Victor César Freitas Godoy, Sara Mataroli de Ribeiro, Ricardo Pereira Povh, Jayme Aparecido Pereira, Ulisses de Pádua Lopera-Barrero, Nelson Mauricio |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Urrea-Rojas, Angela Maria Souza, Felipe Pinheiro de Lima, Ed Christian Suzuki de Yamachita, Andrei Lincoln Pandolfi, Victor César Freitas Godoy, Sara Mataroli de Ribeiro, Ricardo Pereira Povh, Jayme Aparecido Pereira, Ulisses de Pádua Lopera-Barrero, Nelson Mauricio |
dc.subject.por.fl_str_mv |
Colossoma macropomum Conservation Genetic variability Microsatellites SSR. Colossoma macropomum Conservação Microssatélites SSR Variabilidade genética. |
topic |
Colossoma macropomum Conservation Genetic variability Microsatellites SSR. Colossoma macropomum Conservação Microssatélites SSR Variabilidade genética. |
description |
The Amazonian fish Tambaqui (Colossoma macropomum) is the most common native species in Brazil. This species has the highest production rate in the Northern region, especially in the State of Rondônia. The genetic evaluation of Tambaqui is an extremely important to increase productivity in fish farms or improve the adaptability in restocking natural populations. The objective of this study was to evaluate the genetic diversity of three Tambaqui broodstocks in Rondônia, Brazil. Six microsatellite markers were used to analyze a total of 89 breeders collected from three fish farms located in Ji-Paraná (JP), Ouro Preto do Oeste (OP) and Presidente Médici (PM). A total of 37 alleles between 140 and 310 bp were found, including the presence of exclusive and low frequency alleles in the three broodstocks. The average values of observed heterozygosity ranged from 0.404 (PM) to 0.499 (JP). The FIS coefficient values were positive for the three broodstocks, demonstrating a deficit of heterozygotes. The Molecular Variance Analysis (AMOVA) showed greater variation within the stocks than between them. The genetic differentiation was moderate and significant between the stocks, with higher differentiation between JP x PM and lower between OP x PM. The Bayesian analysis designated an optimal value of K = 3 groupings. Although there is moderate genetic diversity between broodstocks, the high FIS indicates a possible decline of diversity in the next generations, and therefore, the incorporation of new breeders is suggested to increase the genetic diversity in the three stocks. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa básica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39339 10.5433/1679-0359.2020v41n6Supl2p3249 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39339 |
identifier_str_mv |
10.5433/1679-0359.2020v41n6Supl2p3249 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39339/28281 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3249-3258 Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3249-3258 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306082736144384 |