Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)

Detalhes bibliográficos
Autor(a) principal: Lopera-Barrero, Nelson Mauricio
Data de Publicação: 2016
Outros Autores: Povh, Jayme Aparecido, Sirol, Rodolfo Nardez, Rodriguez-Rodriguez, Maria del Pilar, Lima, Ed Christian Suzuki de, Poveda-Parra, Angela Rocio, Souza, Felipe Pinheiro de, Murari, Pâmela Juliana Furlan, Otonel, Rodrigo Alejandro Arellano, Ribeiro, Ricardo Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22601
Resumo: The genetic diversity of Piaractus mesopotamicus (pacu) and Leporinus elongatus (piapara) broodstocks used in restocking programs in the rivers Paraná and Paranapanema is analyzed. One hundred and twenty specimens (two broodstocks of each species) from fish ponds in Palotina PR Brazil and in Salto Grande SP Brazil were assessed. Ten primers produced 96 fragments, comprising 68 (70.83%) and 94 (97.92%) polymorphic fragments for P. mesopotamicus and L. elongatus broodstocks, respectively. Differences (p < 0.05) in the frequency of 15 and 27 fragments were detected for each species, without exclusive fragments. Shannon Index (0.347 - 0.572) and the percentage of polymorphic fragments (57.3% - 94.8%) revealed high intra-population genetic variability for all broodstocks. Results of molecular variance analyses (AMOVA) showed that most variations do not lie between the broodstocks but within each broodstock (89%). Genetic (0.088 and 0.142) and identity (0.916 and 0.868) distance rates demonstrated similarity between the broodstocks of each species, corroborated by Fst (0.1023 and 010.27) and Nm (4.18 and 4.33) rates, with a slight genetic difference due to genic flux. High intrapopulation genetic variability and similarity between the broodstocks of each species was also detected, proving a common ancestry.
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spelling Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)Diversidade genética de estoques de Pacu e Piapara em programas de repovoamento nos rios Paraná e Paranapanema (Brasil)BroodstocksGenetic conservationGenetic variabilityLeporinus elongatusPiaractus mesopotamicus.Conservação genéticaLeporinus elongatusEstoques de reprodutoresPiaractus mesopotamicusVariabilidade genética.The genetic diversity of Piaractus mesopotamicus (pacu) and Leporinus elongatus (piapara) broodstocks used in restocking programs in the rivers Paraná and Paranapanema is analyzed. One hundred and twenty specimens (two broodstocks of each species) from fish ponds in Palotina PR Brazil and in Salto Grande SP Brazil were assessed. Ten primers produced 96 fragments, comprising 68 (70.83%) and 94 (97.92%) polymorphic fragments for P. mesopotamicus and L. elongatus broodstocks, respectively. Differences (p < 0.05) in the frequency of 15 and 27 fragments were detected for each species, without exclusive fragments. Shannon Index (0.347 - 0.572) and the percentage of polymorphic fragments (57.3% - 94.8%) revealed high intra-population genetic variability for all broodstocks. Results of molecular variance analyses (AMOVA) showed that most variations do not lie between the broodstocks but within each broodstock (89%). Genetic (0.088 and 0.142) and identity (0.916 and 0.868) distance rates demonstrated similarity between the broodstocks of each species, corroborated by Fst (0.1023 and 010.27) and Nm (4.18 and 4.33) rates, with a slight genetic difference due to genic flux. High intrapopulation genetic variability and similarity between the broodstocks of each species was also detected, proving a common ancestry.O objetivo da pesquisa foi analisar a diversidade genética de estoques de Pacu (Piaractus mesopotamicus) e Piapara (Leporinus elongatus) utilizados em programas de repovoamento dos rios Paraná e Paranapanema. Foram analisados 120 exemplares (dois estoques de cada espécie) de pisciculturas das cidades de Palotina (Paraná) e da cidade de Salto Grande (São Paulo). Os 10 iniciadores produziram 96 fragmentos, dos quais 68 (70,83%) e 94 (97,92%) foram polimórficos para os estoques de P. mesopotamicus e L. elongatus, respectivamente. Foram observadas diferenças (P < 0,05) na frequência de 15 e 27 fragmentos para cada espécie, sem a presença de fragmentos exclusivos. Os valores do índice de Shannon (0,347 a 0,572) e da porcentagem de fragmentos polimórficos (57,3% a 94,8%) mostraram uma alta variabilidade genética intra-populacional para todos os estoques. Os resultados das análises de variância molecular (AMOVA) mostraram que a maior parte da variação está dentro de cada estoque (89%) e não entre os estoques. Os valores da distância (0,088 e 0,142) e identidade (0,916 e 0,868) genética demonstraram que existe similaridade entre os estoques de cada espécie, sendo corroborado pelos valores de Fst (0,1023 e 010,27) e Nm (4,18 e 4,33) que mostraram uma moderada diferenciação genética com presença de fluxo gênico. Foi observada alta variabilidade genética intra-populacional e similaridade entre os estoques de cada espécie demonstrando uma origem em comum.UEL2016-09-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Aplicadaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2260110.5433/1679-0359.2016v37n4Supl1p2365Semina: Ciências Agrárias; Vol. 37 No. 4Supl1 (2016); 2365-2374Semina: Ciências Agrárias; v. 37 n. 4Supl1 (2016); 2365-23741679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22601/19584http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLopera-Barrero, Nelson MauricioPovh, Jayme AparecidoSirol, Rodolfo NardezRodriguez-Rodriguez, Maria del PilarLima, Ed Christian Suzuki dePoveda-Parra, Angela RocioSouza, Felipe Pinheiro deMurari, Pâmela Juliana FurlanOtonel, Rodrigo Alejandro ArellanoRibeiro, Ricardo Pereira2022-11-30T15:00:58Zoai:ojs.pkp.sfu.ca:article/22601Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-30T15:00:58Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
Diversidade genética de estoques de Pacu e Piapara em programas de repovoamento nos rios Paraná e Paranapanema (Brasil)
title Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
spellingShingle Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
Lopera-Barrero, Nelson Mauricio
Broodstocks
Genetic conservation
Genetic variability
Leporinus elongatus
Piaractus mesopotamicus.
Conservação genética
Leporinus elongatus
Estoques de reprodutores
Piaractus mesopotamicus
Variabilidade genética.
title_short Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
title_full Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
title_fullStr Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
title_full_unstemmed Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
title_sort Genetic diversity of pacu and piapara broodstocks in restocking programs in the rivers Paraná and Paranapanema (Brazil)
author Lopera-Barrero, Nelson Mauricio
author_facet Lopera-Barrero, Nelson Mauricio
Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Rodriguez-Rodriguez, Maria del Pilar
Lima, Ed Christian Suzuki de
Poveda-Parra, Angela Rocio
Souza, Felipe Pinheiro de
Murari, Pâmela Juliana Furlan
Otonel, Rodrigo Alejandro Arellano
Ribeiro, Ricardo Pereira
author_role author
author2 Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Rodriguez-Rodriguez, Maria del Pilar
Lima, Ed Christian Suzuki de
Poveda-Parra, Angela Rocio
Souza, Felipe Pinheiro de
Murari, Pâmela Juliana Furlan
Otonel, Rodrigo Alejandro Arellano
Ribeiro, Ricardo Pereira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopera-Barrero, Nelson Mauricio
Povh, Jayme Aparecido
Sirol, Rodolfo Nardez
Rodriguez-Rodriguez, Maria del Pilar
Lima, Ed Christian Suzuki de
Poveda-Parra, Angela Rocio
Souza, Felipe Pinheiro de
Murari, Pâmela Juliana Furlan
Otonel, Rodrigo Alejandro Arellano
Ribeiro, Ricardo Pereira
dc.subject.por.fl_str_mv Broodstocks
Genetic conservation
Genetic variability
Leporinus elongatus
Piaractus mesopotamicus.
Conservação genética
Leporinus elongatus
Estoques de reprodutores
Piaractus mesopotamicus
Variabilidade genética.
topic Broodstocks
Genetic conservation
Genetic variability
Leporinus elongatus
Piaractus mesopotamicus.
Conservação genética
Leporinus elongatus
Estoques de reprodutores
Piaractus mesopotamicus
Variabilidade genética.
description The genetic diversity of Piaractus mesopotamicus (pacu) and Leporinus elongatus (piapara) broodstocks used in restocking programs in the rivers Paraná and Paranapanema is analyzed. One hundred and twenty specimens (two broodstocks of each species) from fish ponds in Palotina PR Brazil and in Salto Grande SP Brazil were assessed. Ten primers produced 96 fragments, comprising 68 (70.83%) and 94 (97.92%) polymorphic fragments for P. mesopotamicus and L. elongatus broodstocks, respectively. Differences (p < 0.05) in the frequency of 15 and 27 fragments were detected for each species, without exclusive fragments. Shannon Index (0.347 - 0.572) and the percentage of polymorphic fragments (57.3% - 94.8%) revealed high intra-population genetic variability for all broodstocks. Results of molecular variance analyses (AMOVA) showed that most variations do not lie between the broodstocks but within each broodstock (89%). Genetic (0.088 and 0.142) and identity (0.916 and 0.868) distance rates demonstrated similarity between the broodstocks of each species, corroborated by Fst (0.1023 and 010.27) and Nm (4.18 and 4.33) rates, with a slight genetic difference due to genic flux. High intrapopulation genetic variability and similarity between the broodstocks of each species was also detected, proving a common ancestry.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Aplicada
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22601
10.5433/1679-0359.2016v37n4Supl1p2365
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22601
identifier_str_mv 10.5433/1679-0359.2016v37n4Supl1p2365
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22601/19584
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 37 No. 4Supl1 (2016); 2365-2374
Semina: Ciências Agrárias; v. 37 n. 4Supl1 (2016); 2365-2374
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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