Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7870 |
Resumo: | The Hymenachne amplexicaulis is a grass, considered a weed in the flooded Rice. Its seeds, measure about three millimeters in length and its panicles can produce a large number of viable seeds. This study aimed to establish the appropriate methodology for evaluate the viability of seeds of H. amplexicaulis using tetrazolium test. The tests, with completely randomized design, were conducted in the Herbology Didactic of Laboratory and Seed Laboratory for Didactic and Research, Universidade Federal de Santa Maria in 2010. For the germination test, treatments seed protected by their glumes and without these (naked) were exposed to potassium nitrate at a concentration of 0.2% or distilled water (control). For the tetrazolium test, naked seeds were immersed directly in water for 6, 12 and 24 hours periods at 23°C ± 1°C. Subsequently, the seeds were incubated in a solution of 2,3,5-triphenyl tetrazolium chloride at a concentration of 0.5%, at this temperature and followed-up the evolution of coloration. The seeds were classified as viable or non-viable, according with parameter predetermined, followed by data analysis. The imbibition of seeds without glumes, for six hours, with subsequent immersion in a solution of tetrazolium 0.5% for four hours was effective in determining the viability of seeds. |
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Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seedsAdequação da metodologia do teste de tetrazólio para sementes de Hymenachne amplexicaulisWest indian marsh grassQuick testViability.Matologia/Tecnologia de sementesCapim-capivaraTeste rápidoViabilidade.Matologia/Tecnologia de SementesThe Hymenachne amplexicaulis is a grass, considered a weed in the flooded Rice. Its seeds, measure about three millimeters in length and its panicles can produce a large number of viable seeds. This study aimed to establish the appropriate methodology for evaluate the viability of seeds of H. amplexicaulis using tetrazolium test. The tests, with completely randomized design, were conducted in the Herbology Didactic of Laboratory and Seed Laboratory for Didactic and Research, Universidade Federal de Santa Maria in 2010. For the germination test, treatments seed protected by their glumes and without these (naked) were exposed to potassium nitrate at a concentration of 0.2% or distilled water (control). For the tetrazolium test, naked seeds were immersed directly in water for 6, 12 and 24 hours periods at 23°C ± 1°C. Subsequently, the seeds were incubated in a solution of 2,3,5-triphenyl tetrazolium chloride at a concentration of 0.5%, at this temperature and followed-up the evolution of coloration. The seeds were classified as viable or non-viable, according with parameter predetermined, followed by data analysis. The imbibition of seeds without glumes, for six hours, with subsequent immersion in a solution of tetrazolium 0.5% for four hours was effective in determining the viability of seeds.O Hymenachne amplexicaulis é uma gramínea considerada invasora da cultura do arroz irrigado. Suas sementes medem cerca de três milímetros de comprimento e uma panícula pode produzir grande número de sementes viáveis. Este trabalho teve por objetivo, estabelecer a metodologia adequada para a identificação da viabilidade de sementes de H. amplexicaulis, usando o teste de tetrazólio. Os ensaios, com delineamento experimental inteiramente casualizado, foram conduzidos no laboratório de Herbologia Professor Loreno Covolo e no laboratório Didático e de Pesquisa em Sementes da Universidade Federal de Santa Maria, em 2010. Para o teste de germinação foram utilizadas as combinações de lotes de sementes sem glumas (nuas) e de sementes protegidas pelos seus envoltórios, com nitrato de potássio (0,2%) ou água, como testemunha. Para o teste de tetrazólio, sementes nuas foram embebidas diretamente em água por períodos de 6, 12 e 24 horas, a temperatura de 23°C ± 1°C. Posteriormente, as sementes foram mantidas em solução de 2,3,5-trifenil cloreto de tetrazólio na concentração de 0,5% durante quatro horas na referida temperatura. A embebição em água das sementes sem glumas, durante seis horas, com posterior imersão em solução de tetrazólio a 0,5% por quatro horas é eficiente na determinação da viabilidade das sementes.UEL2012-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/787010.5433/1679-0359.2012v33n5p1819Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 1819-1824Semina: Ciências Agrárias; v. 33 n. 5 (2012); 1819-18241679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7870/11560Silva, Keli Souza daMachado, Sérgio Luiz de OliveiraMenezes, Nilson Lemos deUrban, Leonardo José KurtzAlves, Marcos Vinícius Palmainfo:eu-repo/semantics/openAccess2015-11-19T18:37:20Zoai:ojs.pkp.sfu.ca:article/7870Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:20Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds Adequação da metodologia do teste de tetrazólio para sementes de Hymenachne amplexicaulis |
title |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
spellingShingle |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds Silva, Keli Souza da West indian marsh grass Quick test Viability. Matologia/Tecnologia de sementes Capim-capivara Teste rápido Viabilidade. Matologia/Tecnologia de Sementes |
title_short |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
title_full |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
title_fullStr |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
title_full_unstemmed |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
title_sort |
Methodology adequation of tetrazolium test for Hymenachne amplexicaulis seeds |
author |
Silva, Keli Souza da |
author_facet |
Silva, Keli Souza da Machado, Sérgio Luiz de Oliveira Menezes, Nilson Lemos de Urban, Leonardo José Kurtz Alves, Marcos Vinícius Palma |
author_role |
author |
author2 |
Machado, Sérgio Luiz de Oliveira Menezes, Nilson Lemos de Urban, Leonardo José Kurtz Alves, Marcos Vinícius Palma |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Keli Souza da Machado, Sérgio Luiz de Oliveira Menezes, Nilson Lemos de Urban, Leonardo José Kurtz Alves, Marcos Vinícius Palma |
dc.subject.por.fl_str_mv |
West indian marsh grass Quick test Viability. Matologia/Tecnologia de sementes Capim-capivara Teste rápido Viabilidade. Matologia/Tecnologia de Sementes |
topic |
West indian marsh grass Quick test Viability. Matologia/Tecnologia de sementes Capim-capivara Teste rápido Viabilidade. Matologia/Tecnologia de Sementes |
description |
The Hymenachne amplexicaulis is a grass, considered a weed in the flooded Rice. Its seeds, measure about three millimeters in length and its panicles can produce a large number of viable seeds. This study aimed to establish the appropriate methodology for evaluate the viability of seeds of H. amplexicaulis using tetrazolium test. The tests, with completely randomized design, were conducted in the Herbology Didactic of Laboratory and Seed Laboratory for Didactic and Research, Universidade Federal de Santa Maria in 2010. For the germination test, treatments seed protected by their glumes and without these (naked) were exposed to potassium nitrate at a concentration of 0.2% or distilled water (control). For the tetrazolium test, naked seeds were immersed directly in water for 6, 12 and 24 hours periods at 23°C ± 1°C. Subsequently, the seeds were incubated in a solution of 2,3,5-triphenyl tetrazolium chloride at a concentration of 0.5%, at this temperature and followed-up the evolution of coloration. The seeds were classified as viable or non-viable, according with parameter predetermined, followed by data analysis. The imbibition of seeds without glumes, for six hours, with subsequent immersion in a solution of tetrazolium 0.5% for four hours was effective in determining the viability of seeds. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7870 10.5433/1679-0359.2012v33n5p1819 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7870 |
identifier_str_mv |
10.5433/1679-0359.2012v33n5p1819 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7870/11560 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 5 (2012); 1819-1824 Semina: Ciências Agrárias; v. 33 n. 5 (2012); 1819-1824 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306064758308864 |