The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44833 |
Resumo: | In this study, the possibility of increasing fermentation efficiency of Saccharomyces cerevisiae on sugarcane bagasse (a type of lignocellulosic waste) was analyzed. Sugarcane bagasse was subjected to hydrothermal and acidic pre-treatment. Next, the enzymatic hydrolysis of raw biomass and each pre-treated biomass was performed using CellicCtec® enzymatic complex to obtain sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate. Next, these were fermented by S. cerevisiae to check if the by-products of enzymatic hydrolysis, furfural and acetic acid had an inhibitory effect on fermentation efficiency. Next, each pre-treated biomass was subjected to detoxification involving activated charcoal. Each detoxified biomass was tested for fermentation efficiency. The lignocellulosic composition for sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate, varied significantly, and were found to be, for cellulose 36.7%, 27.7% and 63.7% respectively; for hemicellulose 22.2%, 4.4% and 12% respectively; and for lignin 21.2%, 27.7% and 28.7% respectively. The presence of furfural and acetic acid had a strong influence on the fermentation efficiency of S. cerevisiae, and affected the consumption of sugars in each biomass by more than 90%. Further, we found that the detoxification process increased fermentation efficiency by 12.7% for the hydrothermal hydrolysate while for the acidic hydrolysate no significant difference was observed. This study showed that fermentation with greater efficiency is viable through the combined use of hydrothermal pre-treatment and detoxification. This combination of methods also causes less pollution as compared with the method involving acid pre-treatment due to the reduced number of effluents produced. |
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Semina. Ciências Agrárias (Online) |
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The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxificationA eficiência da fermentação exibida por Saccharomyces cerevisiae no hidrolisado de bagaço de cana-de-açúcar, analisando os efeitos do pré-tratamento e destoxicaçãoLignocellulosic feedstockinhibitorsethanoldegradation products.In this study, the possibility of increasing fermentation efficiency of Saccharomyces cerevisiae on sugarcane bagasse (a type of lignocellulosic waste) was analyzed. Sugarcane bagasse was subjected to hydrothermal and acidic pre-treatment. Next, the enzymatic hydrolysis of raw biomass and each pre-treated biomass was performed using CellicCtec® enzymatic complex to obtain sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate. Next, these were fermented by S. cerevisiae to check if the by-products of enzymatic hydrolysis, furfural and acetic acid had an inhibitory effect on fermentation efficiency. Next, each pre-treated biomass was subjected to detoxification involving activated charcoal. Each detoxified biomass was tested for fermentation efficiency. The lignocellulosic composition for sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate, varied significantly, and were found to be, for cellulose 36.7%, 27.7% and 63.7% respectively; for hemicellulose 22.2%, 4.4% and 12% respectively; and for lignin 21.2%, 27.7% and 28.7% respectively. The presence of furfural and acetic acid had a strong influence on the fermentation efficiency of S. cerevisiae, and affected the consumption of sugars in each biomass by more than 90%. Further, we found that the detoxification process increased fermentation efficiency by 12.7% for the hydrothermal hydrolysate while for the acidic hydrolysate no significant difference was observed. This study showed that fermentation with greater efficiency is viable through the combined use of hydrothermal pre-treatment and detoxification. This combination of methods also causes less pollution as compared with the method involving acid pre-treatment due to the reduced number of effluents produced.Nesse trabalho avaliou-se a possibilidade de se aumentar a eficiência de fermentação de um hidrolisado de bagaço de cana submetido aos pré-tratamentos hidrotérmico (195 ºC, usando 200 rpm por 10 min) e ácido (0,5% (v/v) de ácido sulfúrico a 121ºC por 15 min) (carga de sólidos de 10% m/v). A hidrólise enzimática do material pré-tratado foi realizada utilizado o complexo enzimático CellicCtec® (60 FPU/gbiomassa seca, tampão citrato a 50 mM e pH 4,8) a 50ºC usando 150 rpm por 72h. Antes do processo de detoxificação, realizou-se um teste com a espécie de Saccharomyces cerevisiae para verificar se os compostos furfural (1 e 4g.L-1) e ácido acético (1 e 5% v/v) exerciam significativa inibição na espécie testada. O processo de detoxificação avaliou a concentração de carvão ativado (1, 3 e 5% m/v) e o tempo do processo (30, 45 e 60 min) a 30 ºC, 150 rpm por 24 h. A composição lignocelulosica da biomassa in natura e pré-tratada (hidrotérmico e ácido) foi para celulose (36,7, 27,7 e 63,7%), hemicelulose (22,2, 4,4 e 12%) e lignina (21,2, 27,7 e 28,7%), respectivamente e com rendimento mássico em torno de 60%. A presença de furfural e ácido acético exibiu forte influência na espécie considerada, chegando a prejudicar em mais de 90% o consumo de açúcares no meio. O processo de destoxificação aumentou 13% a eficiência de fermentação para o hidrolisado obtido hidrotermicamente, enquanto que para o ácido não houve diferença significativa. Obtendo assim uma fermentação com maior eficiência, tecnicamente viável e menos poluente.UEL2022-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4483310.5433/1679-0359.2022v43n5p2155Semina: Ciências Agrárias; Vol. 43 No. 5 (2022); 2155-2170Semina: Ciências Agrárias; v. 43 n. 5 (2022); 2155-21701679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44833/47890Copyright (c) 2022 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGomes, Marcia AndréaSantos-Rocha, Martha Suzana Rodrigues dosBarbosa, Kledson LopesVieira, Rosana CorreiaAntunes, Dellysandra Pamela CôrreaSilva, Carlos Eduardo de FariasAlmeida, Renata Maria Rosas GarciaAlbuquerque, Elaine Christine de Magalhães Cabral2022-11-17T13:29:39Zoai:ojs.pkp.sfu.ca:article/44833Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-17T13:29:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification A eficiência da fermentação exibida por Saccharomyces cerevisiae no hidrolisado de bagaço de cana-de-açúcar, analisando os efeitos do pré-tratamento e destoxicação |
title |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
spellingShingle |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification Gomes, Marcia Andréa Lignocellulosic feedstock inhibitors ethanol degradation products. |
title_short |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
title_full |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
title_fullStr |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
title_full_unstemmed |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
title_sort |
The fermentation efficiency exhibited by Saccharomyces cerevisiae on Sugarcane bagasse hydrolysate, by analyzing the effects of pre-treatment and detoxification |
author |
Gomes, Marcia Andréa |
author_facet |
Gomes, Marcia Andréa Santos-Rocha, Martha Suzana Rodrigues dos Barbosa, Kledson Lopes Vieira, Rosana Correia Antunes, Dellysandra Pamela Côrrea Silva, Carlos Eduardo de Farias Almeida, Renata Maria Rosas Garcia Albuquerque, Elaine Christine de Magalhães Cabral |
author_role |
author |
author2 |
Santos-Rocha, Martha Suzana Rodrigues dos Barbosa, Kledson Lopes Vieira, Rosana Correia Antunes, Dellysandra Pamela Côrrea Silva, Carlos Eduardo de Farias Almeida, Renata Maria Rosas Garcia Albuquerque, Elaine Christine de Magalhães Cabral |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes, Marcia Andréa Santos-Rocha, Martha Suzana Rodrigues dos Barbosa, Kledson Lopes Vieira, Rosana Correia Antunes, Dellysandra Pamela Côrrea Silva, Carlos Eduardo de Farias Almeida, Renata Maria Rosas Garcia Albuquerque, Elaine Christine de Magalhães Cabral |
dc.subject.por.fl_str_mv |
Lignocellulosic feedstock inhibitors ethanol degradation products. |
topic |
Lignocellulosic feedstock inhibitors ethanol degradation products. |
description |
In this study, the possibility of increasing fermentation efficiency of Saccharomyces cerevisiae on sugarcane bagasse (a type of lignocellulosic waste) was analyzed. Sugarcane bagasse was subjected to hydrothermal and acidic pre-treatment. Next, the enzymatic hydrolysis of raw biomass and each pre-treated biomass was performed using CellicCtec® enzymatic complex to obtain sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate. Next, these were fermented by S. cerevisiae to check if the by-products of enzymatic hydrolysis, furfural and acetic acid had an inhibitory effect on fermentation efficiency. Next, each pre-treated biomass was subjected to detoxification involving activated charcoal. Each detoxified biomass was tested for fermentation efficiency. The lignocellulosic composition for sugarcane hydrolysate, hydrothermal hydrolysate and acidic hydrolysate, varied significantly, and were found to be, for cellulose 36.7%, 27.7% and 63.7% respectively; for hemicellulose 22.2%, 4.4% and 12% respectively; and for lignin 21.2%, 27.7% and 28.7% respectively. The presence of furfural and acetic acid had a strong influence on the fermentation efficiency of S. cerevisiae, and affected the consumption of sugars in each biomass by more than 90%. Further, we found that the detoxification process increased fermentation efficiency by 12.7% for the hydrothermal hydrolysate while for the acidic hydrolysate no significant difference was observed. This study showed that fermentation with greater efficiency is viable through the combined use of hydrothermal pre-treatment and detoxification. This combination of methods also causes less pollution as compared with the method involving acid pre-treatment due to the reduced number of effluents produced. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44833 10.5433/1679-0359.2022v43n5p2155 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44833 |
identifier_str_mv |
10.5433/1679-0359.2022v43n5p2155 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44833/47890 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 43 No. 5 (2022); 2155-2170 Semina: Ciências Agrárias; v. 43 n. 5 (2022); 2155-2170 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306056427372544 |