Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12043 |
Resumo: | This study aimed i) to quantify the occurrence of Fusarium spp. and Trichoderma spp. in rhizospheric soil, with and without symptoms of Sudden Death Syndrome (SDS) in eight soybean genotypes; ii) morphologically identify isolates of Fusarium spp. from roots with SDS; iii) evaluate the antagonism between Trichoderma spp. and Fusarium spp. isolates from rhizospheric soil and roots from with and without SDS, respectively; and iv) characterize through the ITS1-5.8S-ITS2 region of rDNA the isolates of Trichoderma spp. with better performance in the direct confrontation. The sampling of soil and roots was performed in an experimental area located in Cruz Alta, RS, Brazil. In the laboratory, serial dilutions of soil samples, counting of the number of Colony Forming Units (UFCs/g-1 of rhizospheric soil) were performed as well as isolation for identification of isolates of Fusarium spp. and Trichoderma spp. and testing of direct confrontation. There were significant differences between the population of Trichoderma spp. in the rhizosphere of plants with and without symptoms of SDS. For the population of Fusarium spp., significant difference was observed only in the rhizosphere of plants without symptoms of SDS. In diseased roots the following species were identified: F. solani, F. avenaceum, F. graminearum, F. oxysporum and F. verticillioides. In the test of direct confrontation, eight isolates of Trichoderma spp. achieved the best performance in the antagonism to Fusarium spp. and Trichoderma spp. from areas with symptoms of SDS had a higher control efficiency in vitro. These isolates showed high similarity to the species of T. koningii agregate. |
id |
UEL-11_4587ed75aff799dc25d05ae6f5a414d6 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/12043 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillageDetecção de Fusarium spp. e Trichoderma spp. e antagonismo de Trichoderma sp. em soja sob plantio diretoGlycine max (L.) MerrilFungiPopulationRoot rotBiological control.PhytopathologyGlycine max (L.) MerrillFungosPopulaçãoPodridão radicularControle biológico.FitopatologiaThis study aimed i) to quantify the occurrence of Fusarium spp. and Trichoderma spp. in rhizospheric soil, with and without symptoms of Sudden Death Syndrome (SDS) in eight soybean genotypes; ii) morphologically identify isolates of Fusarium spp. from roots with SDS; iii) evaluate the antagonism between Trichoderma spp. and Fusarium spp. isolates from rhizospheric soil and roots from with and without SDS, respectively; and iv) characterize through the ITS1-5.8S-ITS2 region of rDNA the isolates of Trichoderma spp. with better performance in the direct confrontation. The sampling of soil and roots was performed in an experimental area located in Cruz Alta, RS, Brazil. In the laboratory, serial dilutions of soil samples, counting of the number of Colony Forming Units (UFCs/g-1 of rhizospheric soil) were performed as well as isolation for identification of isolates of Fusarium spp. and Trichoderma spp. and testing of direct confrontation. There were significant differences between the population of Trichoderma spp. in the rhizosphere of plants with and without symptoms of SDS. For the population of Fusarium spp., significant difference was observed only in the rhizosphere of plants without symptoms of SDS. In diseased roots the following species were identified: F. solani, F. avenaceum, F. graminearum, F. oxysporum and F. verticillioides. In the test of direct confrontation, eight isolates of Trichoderma spp. achieved the best performance in the antagonism to Fusarium spp. and Trichoderma spp. from areas with symptoms of SDS had a higher control efficiency in vitro. These isolates showed high similarity to the species of T. koningii agregate. Este trabalho objetivou i) quantificar a ocorrência de Fusarium spp. e Trichoderma spp. em solo rizosférico, com e sem sintomas de Síndrome da Morte Súbita (SMS), em oito genótipos de soja; ii) identificar morfologicamente Fusarium spp. de raízes; iii) avaliar relações antagônicas entre Trichoderma e Fusarium, isolados de solo rizosférico e raízes de plantas com e sem sintomas de SMS, respectivamente e iv) caracterizar, através da região ITS1-5.8S-ITS2 do rDNA, os isolados de Trichoderma spp. com melhor desempenho no teste de confrontação direta. As coletas de solo e raízes foram realizadas em uma área experimental localizada em Cruz Alta, RS, Brasil. Em laboratório, foram realizadas diluições seriais das amostras de solo, contagem do número de Unidades Formadoras de Colônias (UFCs/g-1 de solo rizosférico) e isolamento para identificação de isolados de Fusarium spp. e Trichoderma spp. e teste de confrontação direta. Houve diferença significativa entre as populações de Trichoderma spp. em solo rizosférico de plantas com e sem sintomas de SMS. Para a população de Fusarium spp., foi constatada diferença significativa apenas em solo rizosférico de plantas sem sintomas da doença. Em raízes doentes, foram identificadas as espécies F. solani, F. avenaceum, F. graminearum, F. oxysporum e F. verticillioides. No teste de confrontação direta, oito isolados de Trichoderma spp. apresentaram o melhor desempenho no antagonismo a Fusarium spp. Os isolados de Trichoderma spp. provenientes de plantas com sintomas de SMS mostraram maior eficiência de controle in vitro. Esses isolados apresentaram alta similaridade com espécies do agregado T. koningii.UEL2013-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionField Empirical ResearchPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1204310.5433/1679-0359.2013v34n6Supl1p3219Semina: Ciências Agrárias; Vol. 34 No. 6Supl1 (2013); 3219-3234Semina: Ciências Agrárias; v. 34 n. 6Supl1 (2013); 3219-32341679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12043/pdf_62Milanesi, Paola MendesBlume, ElenaMuniz, Marlove Fátima BriãoReiniger, Lia Rejane SilveiraAntoniolli, Zaida InêsJunges, EmanueleLupatini, Manoeliinfo:eu-repo/semantics/openAccess2015-11-19T18:36:11Zoai:ojs.pkp.sfu.ca:article/12043Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:36:11Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage Detecção de Fusarium spp. e Trichoderma spp. e antagonismo de Trichoderma sp. em soja sob plantio direto |
title |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
spellingShingle |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage Milanesi, Paola Mendes Glycine max (L.) Merril Fungi Population Root rot Biological control. Phytopathology Glycine max (L.) Merrill Fungos População Podridão radicular Controle biológico. Fitopatologia |
title_short |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
title_full |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
title_fullStr |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
title_full_unstemmed |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
title_sort |
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
author |
Milanesi, Paola Mendes |
author_facet |
Milanesi, Paola Mendes Blume, Elena Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira Antoniolli, Zaida Inês Junges, Emanuele Lupatini, Manoeli |
author_role |
author |
author2 |
Blume, Elena Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira Antoniolli, Zaida Inês Junges, Emanuele Lupatini, Manoeli |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Milanesi, Paola Mendes Blume, Elena Muniz, Marlove Fátima Brião Reiniger, Lia Rejane Silveira Antoniolli, Zaida Inês Junges, Emanuele Lupatini, Manoeli |
dc.subject.por.fl_str_mv |
Glycine max (L.) Merril Fungi Population Root rot Biological control. Phytopathology Glycine max (L.) Merrill Fungos População Podridão radicular Controle biológico. Fitopatologia |
topic |
Glycine max (L.) Merril Fungi Population Root rot Biological control. Phytopathology Glycine max (L.) Merrill Fungos População Podridão radicular Controle biológico. Fitopatologia |
description |
This study aimed i) to quantify the occurrence of Fusarium spp. and Trichoderma spp. in rhizospheric soil, with and without symptoms of Sudden Death Syndrome (SDS) in eight soybean genotypes; ii) morphologically identify isolates of Fusarium spp. from roots with SDS; iii) evaluate the antagonism between Trichoderma spp. and Fusarium spp. isolates from rhizospheric soil and roots from with and without SDS, respectively; and iv) characterize through the ITS1-5.8S-ITS2 region of rDNA the isolates of Trichoderma spp. with better performance in the direct confrontation. The sampling of soil and roots was performed in an experimental area located in Cruz Alta, RS, Brazil. In the laboratory, serial dilutions of soil samples, counting of the number of Colony Forming Units (UFCs/g-1 of rhizospheric soil) were performed as well as isolation for identification of isolates of Fusarium spp. and Trichoderma spp. and testing of direct confrontation. There were significant differences between the population of Trichoderma spp. in the rhizosphere of plants with and without symptoms of SDS. For the population of Fusarium spp., significant difference was observed only in the rhizosphere of plants without symptoms of SDS. In diseased roots the following species were identified: F. solani, F. avenaceum, F. graminearum, F. oxysporum and F. verticillioides. In the test of direct confrontation, eight isolates of Trichoderma spp. achieved the best performance in the antagonism to Fusarium spp. and Trichoderma spp. from areas with symptoms of SDS had a higher control efficiency in vitro. These isolates showed high similarity to the species of T. koningii agregate. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Field Empirical Research Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12043 10.5433/1679-0359.2013v34n6Supl1p3219 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12043 |
identifier_str_mv |
10.5433/1679-0359.2013v34n6Supl1p3219 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12043/pdf_62 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 34 No. 6Supl1 (2013); 3219-3234 Semina: Ciências Agrárias; v. 34 n. 6Supl1 (2013); 3219-3234 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1750315415257481216 |