Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay

Detalhes bibliográficos
Autor(a) principal: Rosa, Claudia Moreno
Data de Publicação: 1999
Outros Autores: Oliveira, Tereza Cristina Rocha Moreira, Hirooka, Elisa Yoko
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/5048
Resumo: The frequency of staphylococcal intoxication requires constant improvements to provide more sensitive and practical assays for enterotoxins detection in foods. Considering that such an advantage is always related to an unexpected events, this view point approaches about recent available resources to reduce the problems associated with new techniques. Emphasis was appointed to the culture formulations for enterotoxin purification, as the remotion of food components which interfire with the most simplied method of enterotoxin detection, the latex agglutination assay- RPLA.  
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spelling Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assayEnterotoxina estafilocócica para produção de padrão e eliminação de interferentes alimentares no método de RPLAStaphylococciEnterotoxinCulture mediumAffinity chromatographyExtraction.EstafilococoEnterotoxinaMeio de culturaCromatografia de afinidadeExtração The frequency of staphylococcal intoxication requires constant improvements to provide more sensitive and practical assays for enterotoxins detection in foods. Considering that such an advantage is always related to an unexpected events, this view point approaches about recent available resources to reduce the problems associated with new techniques. Emphasis was appointed to the culture formulations for enterotoxin purification, as the remotion of food components which interfire with the most simplied method of enterotoxin detection, the latex agglutination assay- RPLA.   A freqüente ocorrência de intoxicação estafilocócica requer desenvolvimento de métodos rápidos de diagnóstico, cada vez mais sensíveis e práticos para a detecção direta de enterotoxinas em alimentos. Considerando que estes métodos são constantemente acompanhados por imprevistos, nesta revisão apresenta-se um ponto de vista sobre os atuais recursos disponíveis, para contornar os problemas oriundos com o lançamento de novas técnicas. Ênfase foi dada aos meios de cultura que facilitam a purificação de enterotoxinas, assim como a eliminação de componentes alimentares no método de aglutinação de látex - RPLA, que consiste de técnica simples com alta sensibilidade para a detecção de enterotoxinas estafilocócicas.    UEL1999-01-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/504810.5433/1679-0359.1999v20n1p71Semina: Ciências Agrárias; Vol. 20 No. 1 (1999); 71-78Semina: Ciências Agrárias; v. 20 n. 1 (1999); 71-781679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/5048/4538Rosa, Claudia MorenoOliveira, Tereza Cristina Rocha MoreiraHirooka, Elisa Yokoinfo:eu-repo/semantics/openAccess2010-04-28T16:23:13Zoai:ojs.pkp.sfu.ca:article/5048Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-04-28T16:23:13Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
Enterotoxina estafilocócica para produção de padrão e eliminação de interferentes alimentares no método de RPLA
title Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
spellingShingle Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
Rosa, Claudia Moreno
Staphylococci
Enterotoxin
Culture medium
Affinity chromatography
Extraction.
Estafilococo
Enterotoxina
Meio de cultura
Cromatografia de afinidade
Extração
title_short Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
title_full Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
title_fullStr Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
title_full_unstemmed Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
title_sort Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay
author Rosa, Claudia Moreno
author_facet Rosa, Claudia Moreno
Oliveira, Tereza Cristina Rocha Moreira
Hirooka, Elisa Yoko
author_role author
author2 Oliveira, Tereza Cristina Rocha Moreira
Hirooka, Elisa Yoko
author2_role author
author
dc.contributor.author.fl_str_mv Rosa, Claudia Moreno
Oliveira, Tereza Cristina Rocha Moreira
Hirooka, Elisa Yoko
dc.subject.por.fl_str_mv Staphylococci
Enterotoxin
Culture medium
Affinity chromatography
Extraction.
Estafilococo
Enterotoxina
Meio de cultura
Cromatografia de afinidade
Extração
topic Staphylococci
Enterotoxin
Culture medium
Affinity chromatography
Extraction.
Estafilococo
Enterotoxina
Meio de cultura
Cromatografia de afinidade
Extração
description The frequency of staphylococcal intoxication requires constant improvements to provide more sensitive and practical assays for enterotoxins detection in foods. Considering that such an advantage is always related to an unexpected events, this view point approaches about recent available resources to reduce the problems associated with new techniques. Emphasis was appointed to the culture formulations for enterotoxin purification, as the remotion of food components which interfire with the most simplied method of enterotoxin detection, the latex agglutination assay- RPLA.  
publishDate 1999
dc.date.none.fl_str_mv 1999-01-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/5048
10.5433/1679-0359.1999v20n1p71
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/5048
identifier_str_mv 10.5433/1679-0359.1999v20n1p71
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/5048/4538
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 20 No. 1 (1999); 71-78
Semina: Ciências Agrárias; v. 20 n. 1 (1999); 71-78
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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