Production and characterization of Fusarium verticillioides exoantigens

Detalhes bibliográficos
Autor(a) principal: Meirelles, Paula Garcia
Data de Publicação: 2006
Outros Autores: Biazon, Luciana, Ono, Mario Augusto, Silva, Adriana Pereira da, Hirooka, Elisa Yoko, Ono, Elisabete Yurie Sataque
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2399
Resumo: Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) is a primary corn pathogen and the main fumonisin producer. This fungus can cause significant economical losses for the farmers, grain processors, animal producers and risk for human and animal health. Several methods for mould detection have been used, however most of these are time-consuming and require trained staff. Otherwise, immunoassays (particularly enzyme-linked immunosorbent assay, or ELISA) provide several advantages and potential for use in rapid quality control. In this work exoantigens from eight F. verticillioides isolates were obtained for further production of polyclonal antibodies. The electrophoretic profile of these antigens showed protein bands with molecular mass ranging from 17 to 170 kDa. The antigens from 3 isolates (97K, 113F and 162A) were selected for polyclonal antibodies production, based on protein concentration and number of bands. Antiserum against F. verticillioides 97K exoantigens, which showed the highest titre in indirect ELISA (1:12.800), has potential for the immunodetection of F. verticillioides.
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spelling Production and characterization of Fusarium verticillioides exoantigensProdução e caracterização de exoantígenos de Fusarium verticillioidesFungiImmunoassayELISAImmunodetectionMycotoxins.FungosImunoensaioELISAImunodetecçãoMicotoxinas.Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) is a primary corn pathogen and the main fumonisin producer. This fungus can cause significant economical losses for the farmers, grain processors, animal producers and risk for human and animal health. Several methods for mould detection have been used, however most of these are time-consuming and require trained staff. Otherwise, immunoassays (particularly enzyme-linked immunosorbent assay, or ELISA) provide several advantages and potential for use in rapid quality control. In this work exoantigens from eight F. verticillioides isolates were obtained for further production of polyclonal antibodies. The electrophoretic profile of these antigens showed protein bands with molecular mass ranging from 17 to 170 kDa. The antigens from 3 isolates (97K, 113F and 162A) were selected for polyclonal antibodies production, based on protein concentration and number of bands. Antiserum against F. verticillioides 97K exoantigens, which showed the highest titre in indirect ELISA (1:12.800), has potential for the immunodetection of F. verticillioides.Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) é um patógeno primário de milho e principal produtor de fumonisinas. Este fungo pode causar perdas econômicas significativas para produtores e processadores de grãos, criadores de animais, além de representar sérios riscos à saúde humana e animal. Diversos métodos para a detecção de fungos têm sido utilizados, porém a maioria demanda tempo e pessoal treinado. Por outro lado, os métodos imunológicos, particularmente os ensaios imunoenzimáticos, apresentam diversas vantagens para o emprego rápido em controle de qualidade. Neste trabalho, foram obtidos exoantígenos de 8 isolados de F. verticillioides para a produção de anticorpos policlonais. O perfil eletroforético dos antígenos apresentou bandas com massas moleculares aparentes variando entre 17 e 170 kDa. Os antígenos de 3 isolados (97K, 113F e 162A), tendo como base a concentração de proteínas e número de bandas, foram escolhidos para a produção de anticorpos policlonais. O soro imune anti-97K, com maior título no ELISA indireto (1:12.800), apresenta potencial para a imunodetecção de Fusarium verticillioides.UEL2006-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/239910.5433/1679-0359.2006v27n1p71Semina: Ciências Agrárias; Vol. 27 No. 1 (2006); 71-80Semina: Ciências Agrárias; v. 27 n. 1 (2006); 71-801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2399/2054Meirelles, Paula GarciaBiazon, LucianaOno, Mario AugustoSilva, Adriana Pereira daHirooka, Elisa YokoOno, Elisabete Yurie Sataqueinfo:eu-repo/semantics/openAccess2010-03-15T16:54:06Zoai:ojs.pkp.sfu.ca:article/2399Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-15T16:54:06Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Production and characterization of Fusarium verticillioides exoantigens
Produção e caracterização de exoantígenos de Fusarium verticillioides
title Production and characterization of Fusarium verticillioides exoantigens
spellingShingle Production and characterization of Fusarium verticillioides exoantigens
Meirelles, Paula Garcia
Fungi
Immunoassay
ELISA
Immunodetection
Mycotoxins.
Fungos
Imunoensaio
ELISA
Imunodetecção
Micotoxinas.
title_short Production and characterization of Fusarium verticillioides exoantigens
title_full Production and characterization of Fusarium verticillioides exoantigens
title_fullStr Production and characterization of Fusarium verticillioides exoantigens
title_full_unstemmed Production and characterization of Fusarium verticillioides exoantigens
title_sort Production and characterization of Fusarium verticillioides exoantigens
author Meirelles, Paula Garcia
author_facet Meirelles, Paula Garcia
Biazon, Luciana
Ono, Mario Augusto
Silva, Adriana Pereira da
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
author_role author
author2 Biazon, Luciana
Ono, Mario Augusto
Silva, Adriana Pereira da
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Meirelles, Paula Garcia
Biazon, Luciana
Ono, Mario Augusto
Silva, Adriana Pereira da
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
dc.subject.por.fl_str_mv Fungi
Immunoassay
ELISA
Immunodetection
Mycotoxins.
Fungos
Imunoensaio
ELISA
Imunodetecção
Micotoxinas.
topic Fungi
Immunoassay
ELISA
Immunodetection
Mycotoxins.
Fungos
Imunoensaio
ELISA
Imunodetecção
Micotoxinas.
description Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) is a primary corn pathogen and the main fumonisin producer. This fungus can cause significant economical losses for the farmers, grain processors, animal producers and risk for human and animal health. Several methods for mould detection have been used, however most of these are time-consuming and require trained staff. Otherwise, immunoassays (particularly enzyme-linked immunosorbent assay, or ELISA) provide several advantages and potential for use in rapid quality control. In this work exoantigens from eight F. verticillioides isolates were obtained for further production of polyclonal antibodies. The electrophoretic profile of these antigens showed protein bands with molecular mass ranging from 17 to 170 kDa. The antigens from 3 isolates (97K, 113F and 162A) were selected for polyclonal antibodies production, based on protein concentration and number of bands. Antiserum against F. verticillioides 97K exoantigens, which showed the highest titre in indirect ELISA (1:12.800), has potential for the immunodetection of F. verticillioides.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2399
10.5433/1679-0359.2006v27n1p71
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2399
identifier_str_mv 10.5433/1679-0359.2006v27n1p71
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2399/2054
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 27 No. 1 (2006); 71-80
Semina: Ciências Agrárias; v. 27 n. 1 (2006); 71-80
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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