Immunoassays: an alternative for toxigenic fungi detection in foods

Detalhes bibliográficos
Autor(a) principal: Meirelles, Paula Garcia
Data de Publicação: 2006
Outros Autores: Biazon, Luciana, Ono, Mario Augusto, Hirooka, Elisa Yoko, Ono, Elisabete Yurie Sataque
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2535
Resumo: Constant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.
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spelling Immunoassays: an alternative for toxigenic fungi detection in foodsImunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentosImmunoassaysToxigenic fungiMould detectionImmunodetection.ImunoensaiosFungos toxigênicosDetecção de fungosImunodetecção.Constant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.O monitoramento constante da contaminação fúngica é imprescindível para assegurar a qualidade e segurança dos alimentos, reduzindo as perdas econômicas, assim como os riscos à saúde humana e animal. Os métodos tradicionais de identificação e detecção de fungos (cultivo em diversos meios, exame microscópico e análises bioquímicas) geralmente consomem muito tempo e exigem pessoal com experiência. Os imunoensaios, particularmente os ensaios imunoenzimáticos, constituem uma alternativa promissora aos métodos tradicionais devido à alta sensibilidade, especificidade, reprodutibilidade e potencial como método rápido de controle de qualidade. Dentre os ensaios imunoenzimáticos, aqueles baseados em exoantígenos são os mais empregados na resolução de problemas taxonômicos, detecção e identificação de fungos toxigênicos. Nesta revisão serão abordados conceitos básicos de imunoensaios, métodos de detecção de fungos, assim como diversos ensaios imunoenzimáticos para a detecção de fungos toxigênicos em alimentos.UEL2006-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/253510.5433/1679-0359.2006v27n4p613Semina: Ciências Agrárias; Vol. 27 No. 4 (2006); 613-628Semina: Ciências Agrárias; v. 27 n. 4 (2006); 613-6281679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2535/2172Meirelles, Paula GarciaBiazon, LucianaOno, Mario AugustoHirooka, Elisa YokoOno, Elisabete Yurie Sataqueinfo:eu-repo/semantics/openAccess2010-03-17T17:57:35Zoai:ojs.pkp.sfu.ca:article/2535Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-17T17:57:35Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Immunoassays: an alternative for toxigenic fungi detection in foods
Imunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentos
title Immunoassays: an alternative for toxigenic fungi detection in foods
spellingShingle Immunoassays: an alternative for toxigenic fungi detection in foods
Meirelles, Paula Garcia
Immunoassays
Toxigenic fungi
Mould detection
Immunodetection.
Imunoensaios
Fungos toxigênicos
Detecção de fungos
Imunodetecção.
title_short Immunoassays: an alternative for toxigenic fungi detection in foods
title_full Immunoassays: an alternative for toxigenic fungi detection in foods
title_fullStr Immunoassays: an alternative for toxigenic fungi detection in foods
title_full_unstemmed Immunoassays: an alternative for toxigenic fungi detection in foods
title_sort Immunoassays: an alternative for toxigenic fungi detection in foods
author Meirelles, Paula Garcia
author_facet Meirelles, Paula Garcia
Biazon, Luciana
Ono, Mario Augusto
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
author_role author
author2 Biazon, Luciana
Ono, Mario Augusto
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Meirelles, Paula Garcia
Biazon, Luciana
Ono, Mario Augusto
Hirooka, Elisa Yoko
Ono, Elisabete Yurie Sataque
dc.subject.por.fl_str_mv Immunoassays
Toxigenic fungi
Mould detection
Immunodetection.
Imunoensaios
Fungos toxigênicos
Detecção de fungos
Imunodetecção.
topic Immunoassays
Toxigenic fungi
Mould detection
Immunodetection.
Imunoensaios
Fungos toxigênicos
Detecção de fungos
Imunodetecção.
description Constant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2535
10.5433/1679-0359.2006v27n4p613
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2535
identifier_str_mv 10.5433/1679-0359.2006v27n4p613
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2535/2172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 27 No. 4 (2006); 613-628
Semina: Ciências Agrárias; v. 27 n. 4 (2006); 613-628
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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