In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28759 |
Resumo: | Six isolates (Ac1Pd, Ac3Pd, Ac4Pd, Ac5Pd, Ac7Pd, and Ac22Pd) of Pediococcus pentosaceus from sheep milk were tested for safety and for probiotic and technological potential. The results showed that none of the isolates were able to produce biogenic amines or virulence factors. The isolates tested showed low hydrophobicities, high auto-aggregation capacities and co-aggregation with L. monocytogenes ATCC 7644, L. sakei ATCC 15521 and E. faecalis ATCC 19444, but none produced ?-galactosidase and bacteriocins. The isolates did not show growth at pH values 3 and 12, while in a pH range from 4 to 10 the growth was variable. In the absence of bile, all the isolates showed growth, with suppression at bile concentrations of 0.1%, 0.3%, 0.6% and 1.0%. In the disc-diffusion test, the isolates tested were resistant to oxacillin, sulfatrimethoprim and vancomycin but were sensitive to chloramphenicol and tetracycline. The isolates showed variable responses to penicillin G and were resistant to most of the drugs tested, except for amoxicillin trihydrate and ibuprofen. All cultures showed a high milk-acidification capacity after 24 hours and none produced exopolysaccharides. The isolates of P. pentosaceus were able to produce diacetyl; however, no culture showed extracellular proteolytic activity and the autolysis varied from 21.3% to 30.5% after 24 h. The isolates grew at NaCl concentrations of 4.0 and 6.0%, but the growth was lower at 10.0%. Finally, all the isolates were found to be safe but had limited application as probiotics and in some technological uses. |
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In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milkAvaliação in vitro do potencial de segurança, probiótico e tecnológico de Pediococcus pentosaceus isolado de leite de ovelhasDairy technologyLactic acid bactériaStarter.Bactérias ácido-láticasCulturas iniciadorasTecnologia do leite.Six isolates (Ac1Pd, Ac3Pd, Ac4Pd, Ac5Pd, Ac7Pd, and Ac22Pd) of Pediococcus pentosaceus from sheep milk were tested for safety and for probiotic and technological potential. The results showed that none of the isolates were able to produce biogenic amines or virulence factors. The isolates tested showed low hydrophobicities, high auto-aggregation capacities and co-aggregation with L. monocytogenes ATCC 7644, L. sakei ATCC 15521 and E. faecalis ATCC 19444, but none produced ?-galactosidase and bacteriocins. The isolates did not show growth at pH values 3 and 12, while in a pH range from 4 to 10 the growth was variable. In the absence of bile, all the isolates showed growth, with suppression at bile concentrations of 0.1%, 0.3%, 0.6% and 1.0%. In the disc-diffusion test, the isolates tested were resistant to oxacillin, sulfatrimethoprim and vancomycin but were sensitive to chloramphenicol and tetracycline. The isolates showed variable responses to penicillin G and were resistant to most of the drugs tested, except for amoxicillin trihydrate and ibuprofen. All cultures showed a high milk-acidification capacity after 24 hours and none produced exopolysaccharides. The isolates of P. pentosaceus were able to produce diacetyl; however, no culture showed extracellular proteolytic activity and the autolysis varied from 21.3% to 30.5% after 24 h. The isolates grew at NaCl concentrations of 4.0 and 6.0%, but the growth was lower at 10.0%. Finally, all the isolates were found to be safe but had limited application as probiotics and in some technological uses.Testes de avaliação de potencial de segurança, probiótico e tecnológicos foram realizados em seis isolados de Pediococcus pentosaceus (Ac1Pd, Ac3Pd, Ac4Pd, Ac5Pd, Ac7Pd, Ac22Pd) provenientes de leite de ovelha. Os resultados obtidos demonstraram que nenhum dos isolados foi capaz de produzir aminas biogênicas ou fatores de virulência. Os isolados testados apresentaram baixa hidrofobicidade, alta capacidade de autoagregação e coagregação com L. monocytogenes ATCC 7644, L. sakei ATCC 15521 e E. faecalis ATCC 19444, porém nenhum produziu ?-galactosidase e bacteriocinas. Não foi observado desenvolvimento dos isolados em pH 3 e 12, sendo que na faixa de pH de 4 a 10 o desenvolvimento foi variável. Na ausência de bile todos os isolados apresentaram desenvolvimento, observando-se supressão quando em concentrações de 0,1%, 0,3%, 0,6% e 1%. No teste de disco-difusão os isolados testados foram resistentes à oxaciclina, sulfatrimetropim e vancomicina, mas foram sensíveis ao cloranfenicol e à tetraciclina e com resultados variáveis para a penicilina G e foram resistentes à maioria dos medicamentos testados, exceto à amoxicilina tri-hidratada e ibuprofeno. Foi observada acidificação do leite somente após 24 h e nenhum produziu exopolissacarídeos. Os isolados de P. pentosaceus foram capazes de produzir diacetil, no entanto, nenhuma cultura apresentou atividade proteolítica extracelular e a produção de autólise foi variada de 21,3% a 30,5%, após 24 h. Os isolados se desenvolveram em concentrações de NaCl a 4% e 6%, porém o desenvolvimento foi menor na concentração de 10%. Por fim, todos os isolados apresentaram boa capacidade de segurança, mas aplicação limitada como probióticos e alguns aspectos de potencial tecnológico.UEL2018-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTestes de potencial de segurança, probiótico e tecnológicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2875910.5433/1679-0359.2018v39n1p113Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 113-132Semina: Ciências Agrárias; v. 39 n. 1 (2018); 113-1321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28759/23047Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFernandes, Mayara LealPerin, Luana MartinsTodorov, Svetoslav DimitrovNero, Luís AugustoAlencar, Ernandes Rodrigues deFerreira, Márcia de Aguiar2022-10-21T11:57:49Zoai:ojs.pkp.sfu.ca:article/28759Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T11:57:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk Avaliação in vitro do potencial de segurança, probiótico e tecnológico de Pediococcus pentosaceus isolado de leite de ovelhas |
title |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
spellingShingle |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk Fernandes, Mayara Leal Dairy technology Lactic acid bactéria Starter. Bactérias ácido-láticas Culturas iniciadoras Tecnologia do leite. |
title_short |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
title_full |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
title_fullStr |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
title_full_unstemmed |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
title_sort |
In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk |
author |
Fernandes, Mayara Leal |
author_facet |
Fernandes, Mayara Leal Perin, Luana Martins Todorov, Svetoslav Dimitrov Nero, Luís Augusto Alencar, Ernandes Rodrigues de Ferreira, Márcia de Aguiar |
author_role |
author |
author2 |
Perin, Luana Martins Todorov, Svetoslav Dimitrov Nero, Luís Augusto Alencar, Ernandes Rodrigues de Ferreira, Márcia de Aguiar |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Mayara Leal Perin, Luana Martins Todorov, Svetoslav Dimitrov Nero, Luís Augusto Alencar, Ernandes Rodrigues de Ferreira, Márcia de Aguiar |
dc.subject.por.fl_str_mv |
Dairy technology Lactic acid bactéria Starter. Bactérias ácido-láticas Culturas iniciadoras Tecnologia do leite. |
topic |
Dairy technology Lactic acid bactéria Starter. Bactérias ácido-láticas Culturas iniciadoras Tecnologia do leite. |
description |
Six isolates (Ac1Pd, Ac3Pd, Ac4Pd, Ac5Pd, Ac7Pd, and Ac22Pd) of Pediococcus pentosaceus from sheep milk were tested for safety and for probiotic and technological potential. The results showed that none of the isolates were able to produce biogenic amines or virulence factors. The isolates tested showed low hydrophobicities, high auto-aggregation capacities and co-aggregation with L. monocytogenes ATCC 7644, L. sakei ATCC 15521 and E. faecalis ATCC 19444, but none produced ?-galactosidase and bacteriocins. The isolates did not show growth at pH values 3 and 12, while in a pH range from 4 to 10 the growth was variable. In the absence of bile, all the isolates showed growth, with suppression at bile concentrations of 0.1%, 0.3%, 0.6% and 1.0%. In the disc-diffusion test, the isolates tested were resistant to oxacillin, sulfatrimethoprim and vancomycin but were sensitive to chloramphenicol and tetracycline. The isolates showed variable responses to penicillin G and were resistant to most of the drugs tested, except for amoxicillin trihydrate and ibuprofen. All cultures showed a high milk-acidification capacity after 24 hours and none produced exopolysaccharides. The isolates of P. pentosaceus were able to produce diacetyl; however, no culture showed extracellular proteolytic activity and the autolysis varied from 21.3% to 30.5% after 24 h. The isolates grew at NaCl concentrations of 4.0 and 6.0%, but the growth was lower at 10.0%. Finally, all the isolates were found to be safe but had limited application as probiotics and in some technological uses. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Testes de potencial de segurança, probiótico e tecnológico |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28759 10.5433/1679-0359.2018v39n1p113 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28759 |
identifier_str_mv |
10.5433/1679-0359.2018v39n1p113 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/28759/23047 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 39 No. 1 (2018); 113-132 Semina: Ciências Agrárias; v. 39 n. 1 (2018); 113-132 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306077641113600 |