Determination of ptaquiloside in cow's milk by HPLC-UV

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Debora da Silva Freitas
Data de Publicação: 2019
Outros Autores: Keller, Kelly Moura, Melo, Marília Martins, Soto-Blanco, Benito
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751
Resumo: Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.
id UEL-11_5d11bc40394d7dd9a78eff6e1850bdce
oai_identifier_str oai:ojs.pkp.sfu.ca:article/33751
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Determination of ptaquiloside in cow's milk by HPLC-UVDeterminação de ptaquilosídeo no leite de vacas por CLAE-UVPteridium aquilinumPteridium arachnoideumPtaquilosideMilkPoisonous plants.Pteridium aquilinumPteridium arachnoideumPtaquilosídeoLeitePlantas tóxicas.Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.Samambaias do gênero Pteridium são reconhecidas como responsáveis por casos de intoxicação em bovinos. As espécies de Pteridium comumente encontradas no Brasil são P. arachnoideum e P. caudatum. O princípio tóxico dessas plantas é o ptaquilosídeo, um glicosídeos norsesquiterpênico, que é um conhecido carcinógeno. O ptaquilosídeo pode ser excretado no leite de vacas que consomem a samambaia, o que representa um potencial risco para quem o consuma. O objetivo do presente trabalho foi desenvolver uma metodologia analítica utilizando cromatografia líquida de alta eficiência (CLAE) com detecção por ultravioleta (UV) para a determinação das concentrações do ptaquilosídeo no leite bovino. Foi desenvolvida com sucesso uma metodologia analítica utilizando CLAE-UV para a determinação das concentrações do ptaquilosídeo no leite, baseada na conversão deste composto em pterosina B e posterior extração utilizando diclorometano. Entretanto, não foi detectada a presença de ptaquilosídeo nas amostras de leite de propriedades com histórico de hematúria enzoótica bovina e que possuíam P. arachnoideum na pastagem.UEL2019-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3375110.5433/1679-0359.2019v40n4p1715Semina: Ciências Agrárias; Vol. 40 No. 4 (2019); 1715-1722Semina: Ciências Agrárias; v. 40 n. 4 (2019); 1715-17221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751/25472Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessRibeiro, Debora da Silva FreitasKeller, Kelly MouraMelo, Marília MartinsSoto-Blanco, Benito2022-10-19T12:58:26Zoai:ojs.pkp.sfu.ca:article/33751Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T12:58:26Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Determination of ptaquiloside in cow's milk by HPLC-UV
Determinação de ptaquilosídeo no leite de vacas por CLAE-UV
title Determination of ptaquiloside in cow's milk by HPLC-UV
spellingShingle Determination of ptaquiloside in cow's milk by HPLC-UV
Ribeiro, Debora da Silva Freitas
Pteridium aquilinum
Pteridium arachnoideum
Ptaquiloside
Milk
Poisonous plants.
Pteridium aquilinum
Pteridium arachnoideum
Ptaquilosídeo
Leite
Plantas tóxicas.
title_short Determination of ptaquiloside in cow's milk by HPLC-UV
title_full Determination of ptaquiloside in cow's milk by HPLC-UV
title_fullStr Determination of ptaquiloside in cow's milk by HPLC-UV
title_full_unstemmed Determination of ptaquiloside in cow's milk by HPLC-UV
title_sort Determination of ptaquiloside in cow's milk by HPLC-UV
author Ribeiro, Debora da Silva Freitas
author_facet Ribeiro, Debora da Silva Freitas
Keller, Kelly Moura
Melo, Marília Martins
Soto-Blanco, Benito
author_role author
author2 Keller, Kelly Moura
Melo, Marília Martins
Soto-Blanco, Benito
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Debora da Silva Freitas
Keller, Kelly Moura
Melo, Marília Martins
Soto-Blanco, Benito
dc.subject.por.fl_str_mv Pteridium aquilinum
Pteridium arachnoideum
Ptaquiloside
Milk
Poisonous plants.
Pteridium aquilinum
Pteridium arachnoideum
Ptaquilosídeo
Leite
Plantas tóxicas.
topic Pteridium aquilinum
Pteridium arachnoideum
Ptaquiloside
Milk
Poisonous plants.
Pteridium aquilinum
Pteridium arachnoideum
Ptaquilosídeo
Leite
Plantas tóxicas.
description Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751
10.5433/1679-0359.2019v40n4p1715
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751
identifier_str_mv 10.5433/1679-0359.2019v40n4p1715
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751/25472
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 4 (2019); 1715-1722
Semina: Ciências Agrárias; v. 40 n. 4 (2019); 1715-1722
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306080276185088