Determination of ptaquiloside in cow's milk by HPLC-UV
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751 |
Resumo: | Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil. |
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Semina. Ciências Agrárias (Online) |
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Determination of ptaquiloside in cow's milk by HPLC-UVDeterminação de ptaquilosídeo no leite de vacas por CLAE-UVPteridium aquilinumPteridium arachnoideumPtaquilosideMilkPoisonous plants.Pteridium aquilinumPteridium arachnoideumPtaquilosídeoLeitePlantas tóxicas.Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.Samambaias do gênero Pteridium são reconhecidas como responsáveis por casos de intoxicação em bovinos. As espécies de Pteridium comumente encontradas no Brasil são P. arachnoideum e P. caudatum. O princípio tóxico dessas plantas é o ptaquilosídeo, um glicosídeos norsesquiterpênico, que é um conhecido carcinógeno. O ptaquilosídeo pode ser excretado no leite de vacas que consomem a samambaia, o que representa um potencial risco para quem o consuma. O objetivo do presente trabalho foi desenvolver uma metodologia analítica utilizando cromatografia líquida de alta eficiência (CLAE) com detecção por ultravioleta (UV) para a determinação das concentrações do ptaquilosídeo no leite bovino. Foi desenvolvida com sucesso uma metodologia analítica utilizando CLAE-UV para a determinação das concentrações do ptaquilosídeo no leite, baseada na conversão deste composto em pterosina B e posterior extração utilizando diclorometano. Entretanto, não foi detectada a presença de ptaquilosídeo nas amostras de leite de propriedades com histórico de hematúria enzoótica bovina e que possuíam P. arachnoideum na pastagem.UEL2019-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3375110.5433/1679-0359.2019v40n4p1715Semina: Ciências Agrárias; Vol. 40 No. 4 (2019); 1715-1722Semina: Ciências Agrárias; v. 40 n. 4 (2019); 1715-17221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751/25472Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessRibeiro, Debora da Silva FreitasKeller, Kelly MouraMelo, Marília MartinsSoto-Blanco, Benito2022-10-19T12:58:26Zoai:ojs.pkp.sfu.ca:article/33751Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T12:58:26Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Determination of ptaquiloside in cow's milk by HPLC-UV Determinação de ptaquilosídeo no leite de vacas por CLAE-UV |
title |
Determination of ptaquiloside in cow's milk by HPLC-UV |
spellingShingle |
Determination of ptaquiloside in cow's milk by HPLC-UV Ribeiro, Debora da Silva Freitas Pteridium aquilinum Pteridium arachnoideum Ptaquiloside Milk Poisonous plants. Pteridium aquilinum Pteridium arachnoideum Ptaquilosídeo Leite Plantas tóxicas. |
title_short |
Determination of ptaquiloside in cow's milk by HPLC-UV |
title_full |
Determination of ptaquiloside in cow's milk by HPLC-UV |
title_fullStr |
Determination of ptaquiloside in cow's milk by HPLC-UV |
title_full_unstemmed |
Determination of ptaquiloside in cow's milk by HPLC-UV |
title_sort |
Determination of ptaquiloside in cow's milk by HPLC-UV |
author |
Ribeiro, Debora da Silva Freitas |
author_facet |
Ribeiro, Debora da Silva Freitas Keller, Kelly Moura Melo, Marília Martins Soto-Blanco, Benito |
author_role |
author |
author2 |
Keller, Kelly Moura Melo, Marília Martins Soto-Blanco, Benito |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Debora da Silva Freitas Keller, Kelly Moura Melo, Marília Martins Soto-Blanco, Benito |
dc.subject.por.fl_str_mv |
Pteridium aquilinum Pteridium arachnoideum Ptaquiloside Milk Poisonous plants. Pteridium aquilinum Pteridium arachnoideum Ptaquilosídeo Leite Plantas tóxicas. |
topic |
Pteridium aquilinum Pteridium arachnoideum Ptaquiloside Milk Poisonous plants. Pteridium aquilinum Pteridium arachnoideum Ptaquilosídeo Leite Plantas tóxicas. |
description |
Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751 10.5433/1679-0359.2019v40n4p1715 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751 |
identifier_str_mv |
10.5433/1679-0359.2019v40n4p1715 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33751/25472 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 4 (2019); 1715-1722 Semina: Ciências Agrárias; v. 40 n. 4 (2019); 1715-1722 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306080276185088 |