Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances

Detalhes bibliográficos
Autor(a) principal: Lozada, Jorge Avelino Rodriguez
Data de Publicação: 2017
Outros Autores: Silveira, Klever Cristiano, Silva, Libério Junio da, Baldotto, Marihus Altoé, Baldotto, Lílian Estrela Borges
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25953
Resumo: The sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and describe the amylolytic, cellulolytic, ligninolytic, proteolytic, and keratinolytic bacteria in the sludge. Sludge samples were collected at the wastewater treatment plant of the Francap SA poultry company. The nutrient contents, C/N ratio, and pH were determined. For the bacterial count, 10 g sludge was diluted in 90 mL saline solution, which was serially diluted to 10-12. Aliquots of 100 µL of each dilution were transferred to selective media for isolation of bacteria that degrade organic substances. The colony-forming units were determined for each culture medium. Individual colonies were purified and characterized morphologically. The sludge contained 9.5, 1.21, and 0.45 dag kg-1 of N, P, and K, respectively. Fifty-two isolates were purified and characterized, with 2.11 × 1012 to 9.55 ×1015 colony-forming units per g sludge. In conclusion, the sludge generated in poultry slaughterhouse wastewater treatment plants is a rich source of organo-mineral constituents and bacteria with biotechnological potential for degrading organic substances.
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spelling Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substancesProspecção de bactérias de lodo de abatedouro de aves com potencial degradador de substâncias orgânicasAgricultural microbiologyOrganic matterSolid waste.Matéria orgânicaMicrobiologia agrícolaResíduos sólidos.The sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and describe the amylolytic, cellulolytic, ligninolytic, proteolytic, and keratinolytic bacteria in the sludge. Sludge samples were collected at the wastewater treatment plant of the Francap SA poultry company. The nutrient contents, C/N ratio, and pH were determined. For the bacterial count, 10 g sludge was diluted in 90 mL saline solution, which was serially diluted to 10-12. Aliquots of 100 µL of each dilution were transferred to selective media for isolation of bacteria that degrade organic substances. The colony-forming units were determined for each culture medium. Individual colonies were purified and characterized morphologically. The sludge contained 9.5, 1.21, and 0.45 dag kg-1 of N, P, and K, respectively. Fifty-two isolates were purified and characterized, with 2.11 × 1012 to 9.55 ×1015 colony-forming units per g sludge. In conclusion, the sludge generated in poultry slaughterhouse wastewater treatment plants is a rich source of organo-mineral constituents and bacteria with biotechnological potential for degrading organic substances.Os lodos produzidos nas estações de tratamento de efluentes de abatedouros são uma fonte de riqueza química, orgânica e microbiológica que precisa ser explorada biotecnologicamente. Objetivou-se neste estudo: (i) realizar a caracterização química do lodo e (ii) isolar, quantificar e caracterizar bactérias amilolíticas, celulolíticas, ligninolíticas, proteolíticas e queratinolíticas do lodo. Amostras de lodo foram coletadas na estação de tratamento de efluentes da empresa avícola FRANCAP S.A. Foram determinados os teores de nutrientes, a relação C/N e o pH. Para a quantificação bacteriana, 10 g de lodo foram diluídos em 90 mL de solução salina e dela, realizaram-se diluições seriadas até 10-12. Alíquotas de 100 µL de cada diluição foram transferidas aos meios seletivos para isolamento de bactérias degradadoras de substâncias orgânicas. Determinaram-se as unidades formadoras de colônia para cada meio de cultivo. Colônias individuais foram purificadas e caracterizadas morfologicamente. O lodo apresentou teores de N, P e K, de 9,5, 1,21 e 0,45 dag kg-1, respectivamente. Foram purificados e caracterizados 52 isolados, as unidades formadoras de colônia variaram entre 2,11 x 1012 a 9,55 x 1015. Conclui-se que os lodos gerados nas estações de tratamento de efluentes de abatedouro de aves são uma fonte de riqueza organo-mineral, além de apresentar bactérias com potencial biotecnológico de degradar substâncias orgânicas.UEL2017-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2595310.5433/1679-0359.2017v38n3p1209Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1209-1216Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1209-12161679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25953/20994Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLozada, Jorge Avelino RodriguezSilveira, Klever CristianoSilva, Libério Junio daBaldotto, Marihus AltoéBaldotto, Lílian Estrela Borges2022-10-24T13:18:18Zoai:ojs.pkp.sfu.ca:article/25953Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T13:18:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
Prospecção de bactérias de lodo de abatedouro de aves com potencial degradador de substâncias orgânicas
title Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
spellingShingle Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
Lozada, Jorge Avelino Rodriguez
Agricultural microbiology
Organic matter
Solid waste.
Matéria orgânica
Microbiologia agrícola
Resíduos sólidos.
title_short Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_full Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_fullStr Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_full_unstemmed Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
title_sort Prospecting for sludge bacteria from a poultry slaughterhouse, with potential for degrading organic substances
author Lozada, Jorge Avelino Rodriguez
author_facet Lozada, Jorge Avelino Rodriguez
Silveira, Klever Cristiano
Silva, Libério Junio da
Baldotto, Marihus Altoé
Baldotto, Lílian Estrela Borges
author_role author
author2 Silveira, Klever Cristiano
Silva, Libério Junio da
Baldotto, Marihus Altoé
Baldotto, Lílian Estrela Borges
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lozada, Jorge Avelino Rodriguez
Silveira, Klever Cristiano
Silva, Libério Junio da
Baldotto, Marihus Altoé
Baldotto, Lílian Estrela Borges
dc.subject.por.fl_str_mv Agricultural microbiology
Organic matter
Solid waste.
Matéria orgânica
Microbiologia agrícola
Resíduos sólidos.
topic Agricultural microbiology
Organic matter
Solid waste.
Matéria orgânica
Microbiologia agrícola
Resíduos sólidos.
description The sludge produced in wastewater treatment plants of slaughterhouses is a rich source of chemical, organic, and microbiological constituents that can be biotechnologically exploited. The purpose of this study was to (i) conduct a chemical analysis of the sludge, and (ii) isolate, quantify, and describe the amylolytic, cellulolytic, ligninolytic, proteolytic, and keratinolytic bacteria in the sludge. Sludge samples were collected at the wastewater treatment plant of the Francap SA poultry company. The nutrient contents, C/N ratio, and pH were determined. For the bacterial count, 10 g sludge was diluted in 90 mL saline solution, which was serially diluted to 10-12. Aliquots of 100 µL of each dilution were transferred to selective media for isolation of bacteria that degrade organic substances. The colony-forming units were determined for each culture medium. Individual colonies were purified and characterized morphologically. The sludge contained 9.5, 1.21, and 0.45 dag kg-1 of N, P, and K, respectively. Fifty-two isolates were purified and characterized, with 2.11 × 1012 to 9.55 ×1015 colony-forming units per g sludge. In conclusion, the sludge generated in poultry slaughterhouse wastewater treatment plants is a rich source of organo-mineral constituents and bacteria with biotechnological potential for degrading organic substances.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25953
10.5433/1679-0359.2017v38n3p1209
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25953
identifier_str_mv 10.5433/1679-0359.2017v38n3p1209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/25953/20994
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 3 (2017); 1209-1216
Semina: Ciências Agrárias; v. 38 n. 3 (2017); 1209-1216
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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