Nematicidal activity of crambe extracts on Meloidogyne spp.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22378 |
Resumo: | Alternative methods for the control of nematodes, such as the use of plant secondary metabolites, can be explored for integrated pest management systems. The objective of this work was to assess the best solvent for obtaining allyl isothiocyanate from Crambe abyssinica leaves, and the effects of this extract on Meloidogyne incognita and M. javanica. Dry leaves of C. abyssinica at 200 mg L-1 were used to prepare extracts by using water (by infusion and grinding), acetone, water + ethanol (hydroalcoholic extraction), methanol, hexane, and chloroform as solvents. Following the evaporation of the solvents, the residue was resuspended in water for use in the experiments. Distilled water and chemical nematicide were used as control treatments. Once the most effective extracts were defined, the following dosages of dried crambe leaves were used: 0, 200, 300, 400, and 500 mg L-1. High performance liquid chromatography (HPLC) was used to quantify the allyl isothiocyanate present in the extracts. After the solvents evaporated, the residues were eluted with water and used in assays with 200 eggs for the hatching test or 200 second stage juveniles (J2) for mobility and mortality tests. The hydroalcoholic extract was the most effective in reducing the hatching of M. incognita and M. javanica juveniles, by 71.6 and 74.4 percentage points, respectively. The mortality of M. incognita and M. javanica in the hydroalcoholic extract was 93.2 and 64.4%, respectively, followed by the methanol extract (17.6 and 34%) and the extract obtained by grinding (9.2 and 28%). The hydroalcoholic extract at 250 mg L-1 showed high nematicidal effect. The HPLC analysis of the extracts revealed that only the methanol and hydroalcoholic extracts had allyl isothiocyanate, indicating that the inhibitory effects on the hatching, mobility, and mortality were not solely attributed to the presence of this compound. |
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Nematicidal activity of crambe extracts on Meloidogyne spp.Atividade nematicida de extratos de crambe sobre Meloidogyne spp.Allyl isothiocyanateAlternative controlCrambe abyssinicaGlucosinolateMeloidogyne incógnitaMeloidogyne javanica.Alil isotiocianatoControle alternativoCrambe abyssinicaGlicosinolatoMeloidogyne incógnitaMeloidogyne javanica.Alternative methods for the control of nematodes, such as the use of plant secondary metabolites, can be explored for integrated pest management systems. The objective of this work was to assess the best solvent for obtaining allyl isothiocyanate from Crambe abyssinica leaves, and the effects of this extract on Meloidogyne incognita and M. javanica. Dry leaves of C. abyssinica at 200 mg L-1 were used to prepare extracts by using water (by infusion and grinding), acetone, water + ethanol (hydroalcoholic extraction), methanol, hexane, and chloroform as solvents. Following the evaporation of the solvents, the residue was resuspended in water for use in the experiments. Distilled water and chemical nematicide were used as control treatments. Once the most effective extracts were defined, the following dosages of dried crambe leaves were used: 0, 200, 300, 400, and 500 mg L-1. High performance liquid chromatography (HPLC) was used to quantify the allyl isothiocyanate present in the extracts. After the solvents evaporated, the residues were eluted with water and used in assays with 200 eggs for the hatching test or 200 second stage juveniles (J2) for mobility and mortality tests. The hydroalcoholic extract was the most effective in reducing the hatching of M. incognita and M. javanica juveniles, by 71.6 and 74.4 percentage points, respectively. The mortality of M. incognita and M. javanica in the hydroalcoholic extract was 93.2 and 64.4%, respectively, followed by the methanol extract (17.6 and 34%) and the extract obtained by grinding (9.2 and 28%). The hydroalcoholic extract at 250 mg L-1 showed high nematicidal effect. The HPLC analysis of the extracts revealed that only the methanol and hydroalcoholic extracts had allyl isothiocyanate, indicating that the inhibitory effects on the hatching, mobility, and mortality were not solely attributed to the presence of this compound.Medidas alternativas de controle de fitonematoides, como o uso de metabólitos secundários produzidos pelas plantas, podem ser exploradas no contexto do manejo integrado em fitossanidade. O objetivo deste trabalho foi verificar quais os melhores solventes para obtenção de alil isotiocianato de folhas de Crambe abyssinica, e o efeito dos mesmos sobre Meloidogyne incognita e Meloidogyne javanica. Folhas secas de crambe na concentração de 200 mg L-1 foram utilizadas para o preparo dos extratos com os solventes água (para os extratos obtidos por infusão e trituração), acetona (cetônico), água + álcool etílico (hidroalcoólico), álcool metílico (metanólico), hexano (hexânico) e clorofórmio (clorofórmico). Após a rotoevaporação dos solventes o resíduo foi ressuspenso em água para utilização nos ensaios. Água destilada e nematicida foram usados como tratamentos controle. Após a seleção dos melhores extratos, os selecionados foram avaliados nas seguintes doses: 0, 200, 300, 400 e 500 mg L-1 de folhas secas de crambe. Para quantificar o alil isotiocianato presente nos extratos foi utilizada cromatografia líquida de alta pressão (HPLC). Para avaliar a eclosão foram utilizados 200 ovos e para motilidade e mortalidade, 200 juvenis de segundo estádio (J2). O extrato hidroalcoólico foi o mais efetivo em reduzir a eclosão de juvenis de M. incognita e M. javanica, com redução de 71.6 e 74.4 pontos percentuais, respectivamente. A mortalidade de M. incognita e M. javanica para o extrato hidroalcoólico foi de 93.2% e 64.4%, respectivamente, seguido do extrato metanólico (17.6% e 34%) e do extrato obtido por trituração (9.2% e 28%), respectivamente. Para o extrato hidroalcoólico, a concentração com melhor efeito nematicida foi de 250 mg L-1. Dos extratos analisados por HPLC, somente o metanólico e o hidroalcoólico apresentaram alil isotiocianato, indicando que os efeitos inibitórios da eclosão, motilidade e mortalidade não foram devidos apenas à presença desse composto.UEL2016-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionartigoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2237810.5433/1679-0359.2016v37n4p1857Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 1857-1870Semina: Ciências Agrárias; v. 37 n. 4 (2016); 1857-18701679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22378/19635http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessColtro-Roncato, SidianeStangarlin, José RenatoGonçalves Jr., Affonso CelsoKuhn, Odair JoséGonçalves, Edilaine Della ValentinaDildey, Omari Dangelo ForlinFlores, Éder Lisandro de Moraes2022-11-30T16:30:31Zoai:ojs.pkp.sfu.ca:article/22378Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-30T16:30:31Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Nematicidal activity of crambe extracts on Meloidogyne spp. Atividade nematicida de extratos de crambe sobre Meloidogyne spp. |
title |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
spellingShingle |
Nematicidal activity of crambe extracts on Meloidogyne spp. Coltro-Roncato, Sidiane Allyl isothiocyanate Alternative control Crambe abyssinica Glucosinolate Meloidogyne incógnita Meloidogyne javanica. Alil isotiocianato Controle alternativo Crambe abyssinica Glicosinolato Meloidogyne incógnita Meloidogyne javanica. |
title_short |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
title_full |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
title_fullStr |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
title_full_unstemmed |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
title_sort |
Nematicidal activity of crambe extracts on Meloidogyne spp. |
author |
Coltro-Roncato, Sidiane |
author_facet |
Coltro-Roncato, Sidiane Stangarlin, José Renato Gonçalves Jr., Affonso Celso Kuhn, Odair José Gonçalves, Edilaine Della Valentina Dildey, Omari Dangelo Forlin Flores, Éder Lisandro de Moraes |
author_role |
author |
author2 |
Stangarlin, José Renato Gonçalves Jr., Affonso Celso Kuhn, Odair José Gonçalves, Edilaine Della Valentina Dildey, Omari Dangelo Forlin Flores, Éder Lisandro de Moraes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Coltro-Roncato, Sidiane Stangarlin, José Renato Gonçalves Jr., Affonso Celso Kuhn, Odair José Gonçalves, Edilaine Della Valentina Dildey, Omari Dangelo Forlin Flores, Éder Lisandro de Moraes |
dc.subject.por.fl_str_mv |
Allyl isothiocyanate Alternative control Crambe abyssinica Glucosinolate Meloidogyne incógnita Meloidogyne javanica. Alil isotiocianato Controle alternativo Crambe abyssinica Glicosinolato Meloidogyne incógnita Meloidogyne javanica. |
topic |
Allyl isothiocyanate Alternative control Crambe abyssinica Glucosinolate Meloidogyne incógnita Meloidogyne javanica. Alil isotiocianato Controle alternativo Crambe abyssinica Glicosinolato Meloidogyne incógnita Meloidogyne javanica. |
description |
Alternative methods for the control of nematodes, such as the use of plant secondary metabolites, can be explored for integrated pest management systems. The objective of this work was to assess the best solvent for obtaining allyl isothiocyanate from Crambe abyssinica leaves, and the effects of this extract on Meloidogyne incognita and M. javanica. Dry leaves of C. abyssinica at 200 mg L-1 were used to prepare extracts by using water (by infusion and grinding), acetone, water + ethanol (hydroalcoholic extraction), methanol, hexane, and chloroform as solvents. Following the evaporation of the solvents, the residue was resuspended in water for use in the experiments. Distilled water and chemical nematicide were used as control treatments. Once the most effective extracts were defined, the following dosages of dried crambe leaves were used: 0, 200, 300, 400, and 500 mg L-1. High performance liquid chromatography (HPLC) was used to quantify the allyl isothiocyanate present in the extracts. After the solvents evaporated, the residues were eluted with water and used in assays with 200 eggs for the hatching test or 200 second stage juveniles (J2) for mobility and mortality tests. The hydroalcoholic extract was the most effective in reducing the hatching of M. incognita and M. javanica juveniles, by 71.6 and 74.4 percentage points, respectively. The mortality of M. incognita and M. javanica in the hydroalcoholic extract was 93.2 and 64.4%, respectively, followed by the methanol extract (17.6 and 34%) and the extract obtained by grinding (9.2 and 28%). The hydroalcoholic extract at 250 mg L-1 showed high nematicidal effect. The HPLC analysis of the extracts revealed that only the methanol and hydroalcoholic extracts had allyl isothiocyanate, indicating that the inhibitory effects on the hatching, mobility, and mortality were not solely attributed to the presence of this compound. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artigo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22378 10.5433/1679-0359.2016v37n4p1857 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22378 |
identifier_str_mv |
10.5433/1679-0359.2016v37n4p1857 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22378/19635 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 1857-1870 Semina: Ciências Agrárias; v. 37 n. 4 (2016); 1857-1870 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306074420936704 |