Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230 |
Resumo: | We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation. |
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Semina. Ciências Agrárias (Online) |
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Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?Cabra incubadora: os oócitos bovinos podem ser maturados no corno uterino de uma cabra?COCsBovine oocytesIn vitro maturationEx-situ maturationNonsurgical embryo transfer techniqueGoat.COCsOócitos bovinoMaturação in vitroMaturação ex-situTécnica de transferência de embriões não cirúrgicosCabra.We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation.Utilizamos uma cabra como incubadora viva, juntamente com técnicas de transferência de embriões não cirúrgicas associadas, para realizar a maturação ex situ (in vivo) de oócitos bovinos. Os complexos cumulus-oócitos bovinos (COCs), aspirados de folículos de 3-8 mm de ovários de abatedouro foram aleatoriamente divididos em dois grupos para maturação in vitro (IVM; n = 38) e ex situ (ESM; n = 40). A MIV foi realizada por um período de 24 h no meio TCM-199, a 38,5 ºC, e com 5% de CO2 em umidade máxima. Para o ESM, uma cabra nulípara pré-sincronizada (12 meses de idade) recebeu 40 COCs imaturos no ápice do corno uterino, por via transcervical. Após 24 h as estruturas foram recuperadas através de lavagem uterina. Análises de maturação nuclear e quantificação de lipídios foram realizadas em oócitos de ambos os grupos. A intensidade de fluorescente foi comparada usando o teste t de Student. Quarenta e sete por cento das estruturas foram recuperadas após lavagem uterina (19/40). A taxa de maturação nuclear foi de 94,5% (18/19) e 81,6% (31/38) para os grupos ESM e IVM, respectivamente. Os COCs maturados in vitro continham mais gotículas lipídicas, expressos como uma quantidade maior (p < 0,05) da luz fluorescente emitida do que os COCs ex situ (858 ± 73 vs 550 ± 64 unidades de fluorescência arbitrárias, respectivamente). Este é o primeiro relatório a associar técnicas de transferência de embriões não cirúrgicas e uma cabra como incubadora viva, para a maturação de oócitos bovinos. Conclui-se que os oócitos bovinos podem progredir meioticamente no corno uterino de uma cabra, e que a transferência transcervical de oócitos bovinos para o útero de cabra poderia ser uma alternativa à maturação nuclear, visando à redução de lipídios.UEL2019-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3523010.5433/1679-0359.2019v40n6Supl3p3789Semina: Ciências Agrárias; Vol. 40 No. 6Supl3 (2019); 3789-3796Semina: Ciências Agrárias; v. 40 n. 6Supl3 (2019); 3789-37961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230/26260Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFonseca, Jeferson Fereira daBatista, Ribrio Ivan Tavares PereiraTrevizan, Juliane TeramachiSouza-Fabjan, Joanna Maria GonçalvesBrandão, Felipe ZandonadiCamargo, Luiz Sérgio Almeida2022-10-10T15:46:54Zoai:ojs.pkp.sfu.ca:article/35230Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T15:46:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? Cabra incubadora: os oócitos bovinos podem ser maturados no corno uterino de uma cabra? |
title |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
spellingShingle |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? Fonseca, Jeferson Fereira da COCs Bovine oocytes In vitro maturation Ex-situ maturation Nonsurgical embryo transfer technique Goat. COCs Oócitos bovino Maturação in vitro Maturação ex-situ Técnica de transferência de embriões não cirúrgicos Cabra. |
title_short |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
title_full |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
title_fullStr |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
title_full_unstemmed |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
title_sort |
Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat? |
author |
Fonseca, Jeferson Fereira da |
author_facet |
Fonseca, Jeferson Fereira da Batista, Ribrio Ivan Tavares Pereira Trevizan, Juliane Teramachi Souza-Fabjan, Joanna Maria Gonçalves Brandão, Felipe Zandonadi Camargo, Luiz Sérgio Almeida |
author_role |
author |
author2 |
Batista, Ribrio Ivan Tavares Pereira Trevizan, Juliane Teramachi Souza-Fabjan, Joanna Maria Gonçalves Brandão, Felipe Zandonadi Camargo, Luiz Sérgio Almeida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca, Jeferson Fereira da Batista, Ribrio Ivan Tavares Pereira Trevizan, Juliane Teramachi Souza-Fabjan, Joanna Maria Gonçalves Brandão, Felipe Zandonadi Camargo, Luiz Sérgio Almeida |
dc.subject.por.fl_str_mv |
COCs Bovine oocytes In vitro maturation Ex-situ maturation Nonsurgical embryo transfer technique Goat. COCs Oócitos bovino Maturação in vitro Maturação ex-situ Técnica de transferência de embriões não cirúrgicos Cabra. |
topic |
COCs Bovine oocytes In vitro maturation Ex-situ maturation Nonsurgical embryo transfer technique Goat. COCs Oócitos bovino Maturação in vitro Maturação ex-situ Técnica de transferência de embriões não cirúrgicos Cabra. |
description |
We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230 10.5433/1679-0359.2019v40n6Supl3p3789 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl3p3789 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35230/26260 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl3 (2019); 3789-3796 Semina: Ciências Agrárias; v. 40 n. 6Supl3 (2019); 3789-3796 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306080997605376 |