Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C

Detalhes bibliográficos
Autor(a) principal: Sampaio, Breno Fernandes Barreto
Data de Publicação: 2020
Outros Autores: Nogueira, Bruno Gomes, Souza, Maria Inês Lenz, Costa-e-Silva, Eliane Vianna da, Neto Zúccari, Carmem Estefânia Serra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36068
Resumo: Plasma membrane composition has impact on phase transition from liquid crystal to gel state of cooled sperm cell. The incorporation of polyunsaturated fatty acids increases its fluidity and can contribute to sperm motility. The aim of this study was to compare the effect of adding docosahexaenoic acid (DHA) and ?-tocopherol (?-Toh) to the cooling extender, singly or combined, to the equine sperm parameters, submitted to cooling, up to 72 hours. Two ejaculates of ten stallions collected with artificial vagina were used, and evaluated for motility, plasma membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation, according to the following treatments: C; DHA; ?-Toh; DHA/?-Toh; EtOH 100: and EtOH 140 (corresponding to control; 10 ng mL-1 of DHA; 2 mM of ?-Toh; : 10 ng mL-1 of DHA + 2 mM of ?-Toh; 100 µL of ethanol and 140 µL of ethanol respectively). DHA treatment showed higher motility (68.2 ± 12.3; p < 0.05) when compared to control (62.1 ± 16.2), DHA/?-Toh (61.3 ± 12.7) and EtOH (58.1 ± 8.6) groups. In lipid peroxidation assay, the control group showed 2,506.2 ± 796.4 ng of MDA 108 spermatozoa-1, being significantly higher (p < 0.05) than the groups treated with DHA (2,036.0 ± 687.0), ?-Toh (1,890.8 ± 749.5) and DHA/?-Toh (1,821.1 ± 627.2). In conclusion, ?-Toh was effective in diminishing lipid peroxidation of equine sperm subjected to cooling, and DHA improved sperm motility and, in spite of being a polyunsaturated fatty acid with high susceptibility to peroxidation, reduced lipid peroxidation.
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spelling Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°CEfeitos da adição de ácido docosahexaenoico e ?-tocoferol na qualidade do espermatozoide equino armazenados a 5°CAntioxidantDHAStallionVitamin E.AntioxidanteDHAGaranhãoVitamina E.Plasma membrane composition has impact on phase transition from liquid crystal to gel state of cooled sperm cell. The incorporation of polyunsaturated fatty acids increases its fluidity and can contribute to sperm motility. The aim of this study was to compare the effect of adding docosahexaenoic acid (DHA) and ?-tocopherol (?-Toh) to the cooling extender, singly or combined, to the equine sperm parameters, submitted to cooling, up to 72 hours. Two ejaculates of ten stallions collected with artificial vagina were used, and evaluated for motility, plasma membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation, according to the following treatments: C; DHA; ?-Toh; DHA/?-Toh; EtOH 100: and EtOH 140 (corresponding to control; 10 ng mL-1 of DHA; 2 mM of ?-Toh; : 10 ng mL-1 of DHA + 2 mM of ?-Toh; 100 µL of ethanol and 140 µL of ethanol respectively). DHA treatment showed higher motility (68.2 ± 12.3; p < 0.05) when compared to control (62.1 ± 16.2), DHA/?-Toh (61.3 ± 12.7) and EtOH (58.1 ± 8.6) groups. In lipid peroxidation assay, the control group showed 2,506.2 ± 796.4 ng of MDA 108 spermatozoa-1, being significantly higher (p < 0.05) than the groups treated with DHA (2,036.0 ± 687.0), ?-Toh (1,890.8 ± 749.5) and DHA/?-Toh (1,821.1 ± 627.2). In conclusion, ?-Toh was effective in diminishing lipid peroxidation of equine sperm subjected to cooling, and DHA improved sperm motility and, in spite of being a polyunsaturated fatty acid with high susceptibility to peroxidation, reduced lipid peroxidation.A composição da membrana plasmática tem impacto na transição de fase do estado cristal líquido para o estado gel das células espermáticas submetidas à refrigeração. A incorporação de ácidos graxos poliinsaturados aumenta sua fluidez e pode contribuir para a motilidade espermática. O objetivo deste estudo foi comparar o efeito da adição de ácido docosahexaenóico (DHA) e ?-tocoferol (?-Toh) ao diluidor de refrigeração, isoladamente ou combinado, aos parâmetros do sêmen equino, submetidos à refrigeração por até 72 horas. Foram utilizados dois ejaculados de dez garanhões coletados com vagina artificial e avaliados quanto à motilidade, integridade da membrana plasmática, fragmentação da cromatina, atividade mitocondrial e peroxidação lipídica, de acordo com os seguintes tratamentos: C; DHA; a-Toh; DHA / a-Toh; EtOH 100: e EtOH 140 (correspondentes ao controle; 10 ng mL-1 de DHA; 2 mM de ?-Toh;: 10 ng mL-1 de DHA + 2 mM de ?-Toh; 100 µL de etanol e 140 µL de etanol, respectivamente). O tratamento com DHA mostrou maior motilidade (68,2 ± 12,3; p < 0,05) quando comparado aos grupos controle (62,1 ± 16,2), DHA/?-Toh (61,3 ± 12,7) e EtOH (58,1 ± 8,6). Na análise de peroxidação lipídica, o grupo controle mostrou 2.506,2 ± 796,4 ng de MDA 108 espermatozoides-1, sendo significativamente maior (p < 0,05) do que os grupos tratados com DHA (2.036,0 ± 687,0), ?-Toh (1.890,8 ± 749,5) e DHA / a-Toh (1.821,1 ± 627,2). Em conclusão, ?-Toh foi eficaz na diminuição da peroxidação lipídica de espermatozoide equino submetido ao resfriamento, e o DHA melhorou a motilidade espermática e, apesar de ser um ácido graxo poliinsaturado com alta suscetibilidade à peroxidação, reduziu a peroxidação lipídicaUEL2020-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3606810.5433/1679-0359.2020v41n1p167Semina: Ciências Agrárias; Vol. 41 No. 1 (2020); 167-180Semina: Ciências Agrárias; v. 41 n. 1 (2020); 167-1801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36068/26498Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSampaio, Breno Fernandes BarretoNogueira, Bruno GomesSouza, Maria Inês LenzCosta-e-Silva, Eliane Vianna daNeto Zúccari, Carmem Estefânia Serra2022-10-10T14:35:24Zoai:ojs.pkp.sfu.ca:article/36068Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T14:35:24Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
Efeitos da adição de ácido docosahexaenoico e ?-tocoferol na qualidade do espermatozoide equino armazenados a 5°C
title Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
spellingShingle Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
Sampaio, Breno Fernandes Barreto
Antioxidant
DHA
Stallion
Vitamin E.
Antioxidante
DHA
Garanhão
Vitamina E.
title_short Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
title_full Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
title_fullStr Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
title_full_unstemmed Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
title_sort Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C
author Sampaio, Breno Fernandes Barreto
author_facet Sampaio, Breno Fernandes Barreto
Nogueira, Bruno Gomes
Souza, Maria Inês Lenz
Costa-e-Silva, Eliane Vianna da
Neto Zúccari, Carmem Estefânia Serra
author_role author
author2 Nogueira, Bruno Gomes
Souza, Maria Inês Lenz
Costa-e-Silva, Eliane Vianna da
Neto Zúccari, Carmem Estefânia Serra
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sampaio, Breno Fernandes Barreto
Nogueira, Bruno Gomes
Souza, Maria Inês Lenz
Costa-e-Silva, Eliane Vianna da
Neto Zúccari, Carmem Estefânia Serra
dc.subject.por.fl_str_mv Antioxidant
DHA
Stallion
Vitamin E.
Antioxidante
DHA
Garanhão
Vitamina E.
topic Antioxidant
DHA
Stallion
Vitamin E.
Antioxidante
DHA
Garanhão
Vitamina E.
description Plasma membrane composition has impact on phase transition from liquid crystal to gel state of cooled sperm cell. The incorporation of polyunsaturated fatty acids increases its fluidity and can contribute to sperm motility. The aim of this study was to compare the effect of adding docosahexaenoic acid (DHA) and ?-tocopherol (?-Toh) to the cooling extender, singly or combined, to the equine sperm parameters, submitted to cooling, up to 72 hours. Two ejaculates of ten stallions collected with artificial vagina were used, and evaluated for motility, plasma membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation, according to the following treatments: C; DHA; ?-Toh; DHA/?-Toh; EtOH 100: and EtOH 140 (corresponding to control; 10 ng mL-1 of DHA; 2 mM of ?-Toh; : 10 ng mL-1 of DHA + 2 mM of ?-Toh; 100 µL of ethanol and 140 µL of ethanol respectively). DHA treatment showed higher motility (68.2 ± 12.3; p < 0.05) when compared to control (62.1 ± 16.2), DHA/?-Toh (61.3 ± 12.7) and EtOH (58.1 ± 8.6) groups. In lipid peroxidation assay, the control group showed 2,506.2 ± 796.4 ng of MDA 108 spermatozoa-1, being significantly higher (p < 0.05) than the groups treated with DHA (2,036.0 ± 687.0), ?-Toh (1,890.8 ± 749.5) and DHA/?-Toh (1,821.1 ± 627.2). In conclusion, ?-Toh was effective in diminishing lipid peroxidation of equine sperm subjected to cooling, and DHA improved sperm motility and, in spite of being a polyunsaturated fatty acid with high susceptibility to peroxidation, reduced lipid peroxidation.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36068
10.5433/1679-0359.2020v41n1p167
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36068
identifier_str_mv 10.5433/1679-0359.2020v41n1p167
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36068/26498
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 1 (2020); 167-180
Semina: Ciências Agrárias; v. 41 n. 1 (2020); 167-180
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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