Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Luciane de
Data de Publicação: 2020
Outros Autores: Pigatto, Anita Marchionatti, Pigatto, João Antonio Tadeu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38486
Resumo: The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.
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spelling Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation mediumAvaliação do endotélio da córnea de equinos (Equus cabbalus) acondicionado em meio de preservação de córnea Eusol-C®Corneal transplantation. HorsesEndotheliumCorneal storageCell viability.Transplante de córneaCavalosEndotélio.Preservação da córnea. Viabilidade celular.The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.Objetivou-se avaliar a manutenção do endotélio da córnea de equinos em meio de preservação a frio EUSOL-C® em diferentes períodos de acondicionamento (sete e 14 dias) utilizando microscopia eletrônica de varredura. Foram avaliados 40 bulbos oculares de 20 equinos. Os bulbos oculares foram divididos em quatro grupos (G1, G2, G3 e G4), nos quais: G1 foi composto por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante sete dias; o G3 foi formado por 10 botões corneais acondicionados em meio de preservação para córnea Eusol-C® durante 14 dias. O G2 e G4 foram formados pelas córneas controle armazenadas imediatamente em glutaraldeído. A média da perda celular observada no G1 foi de 7,62%, no G2 foi de 7,04 %, no G3 foi de 9,12% e o no G4 foi de 7,16%. Não foram observadas diferenças estatisticamente significativas entre os quatro grupos. Foi possível concluir que o meio de preservação hipotérmico Eusol-C® proporcionou de forma satisfatória a preservação do endotélio da córnea na espécie equina durante o período de até 14 dias.UEL2020-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontrabalho científicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3848610.5433/1679-0359.2020v41n6Supl2p3155Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3155-3164Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3155-31641679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38486/28269Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAlbuquerque, Luciane dePigatto, Anita MarchionattiPigatto, João Antonio Tadeu2022-10-04T16:41:34Zoai:ojs.pkp.sfu.ca:article/38486Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T16:41:34Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
Avaliação do endotélio da córnea de equinos (Equus cabbalus) acondicionado em meio de preservação de córnea Eusol-C®
title Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
spellingShingle Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
Albuquerque, Luciane de
Corneal transplantation. Horses
Endothelium
Corneal storage
Cell viability.
Transplante de córnea
Cavalos
Endotélio.
Preservação da córnea
. Viabilidade celular.
title_short Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
title_full Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
title_fullStr Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
title_full_unstemmed Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
title_sort Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
author Albuquerque, Luciane de
author_facet Albuquerque, Luciane de
Pigatto, Anita Marchionatti
Pigatto, João Antonio Tadeu
author_role author
author2 Pigatto, Anita Marchionatti
Pigatto, João Antonio Tadeu
author2_role author
author
dc.contributor.author.fl_str_mv Albuquerque, Luciane de
Pigatto, Anita Marchionatti
Pigatto, João Antonio Tadeu
dc.subject.por.fl_str_mv Corneal transplantation. Horses
Endothelium
Corneal storage
Cell viability.
Transplante de córnea
Cavalos
Endotélio.
Preservação da córnea
. Viabilidade celular.
topic Corneal transplantation. Horses
Endothelium
Corneal storage
Cell viability.
Transplante de córnea
Cavalos
Endotélio.
Preservação da córnea
. Viabilidade celular.
description The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
trabalho científico
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38486
10.5433/1679-0359.2020v41n6Supl2p3155
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38486
identifier_str_mv 10.5433/1679-0359.2020v41n6Supl2p3155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38486/28269
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3155-3164
Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3155-3164
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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