Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae

Detalhes bibliográficos
Autor(a) principal: Victor, Gustavo
Data de Publicação: 2014
Outros Autores: Nosé, Walton [UNIFESP], Faria e Sousa, Sidney Julio de, Pineda, Roberto, Alves, Milton Ruiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.5935/0034-7280.20140017
http://repositorio.unifesp.br/handle/11600/8360
Resumo: Purpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500µm). The laser cuts were made using an LDV® femtosecond laser in a Ziemer® anterior chamber. To obtain a better edge, the lamellae were trephined with an 8mm trephine (Katena®). The central corneal thickness and the anterior lamellae were measured using a Mitutoyo® thickness gauge with an accuracy of 0.001mm. Results: The central thickness of the 25 corneas ranged from 500 to 705µm (mean, 584 ± 51µm). The thickness of the anterior lamellae ranged from 420 to 480µm (mean, 455 ± 12.7µm). The anterior lamellae diameters were 7.90 ± 0.1mm, and all laser cuts were round. The lamellar interfaces appeared regular by surgical microscopy. There were no cases of inter-lamellar adhesion. Conclusion: The LDV® femtosecond laser appears to be a safe and reliable instrument for cutting deep anterior lamellae from donor corneoscleral buttons. Minimal variation in donor lamellar depth with the laser will be useful for creating donor corneal tissue for deeper anterior lamellar keratoplasty or endothelial keratoplasty surgery or both from a single donor cornea.
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spelling Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellaeLaser de femtossegundo de baixa energia e alta frequência na confecção de lamelas corneanas doadorasCorneaCorneal transplantationCorneal pachymetryEndotheliumLasersCórneaTransplante de córneaPaquimetria corneanaLasersEndotélioPurpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500µm). The laser cuts were made using an LDV® femtosecond laser in a Ziemer® anterior chamber. To obtain a better edge, the lamellae were trephined with an 8mm trephine (Katena®). The central corneal thickness and the anterior lamellae were measured using a Mitutoyo® thickness gauge with an accuracy of 0.001mm. Results: The central thickness of the 25 corneas ranged from 500 to 705µm (mean, 584 ± 51µm). The thickness of the anterior lamellae ranged from 420 to 480µm (mean, 455 ± 12.7µm). The anterior lamellae diameters were 7.90 ± 0.1mm, and all laser cuts were round. The lamellar interfaces appeared regular by surgical microscopy. There were no cases of inter-lamellar adhesion. Conclusion: The LDV® femtosecond laser appears to be a safe and reliable instrument for cutting deep anterior lamellae from donor corneoscleral buttons. Minimal variation in donor lamellar depth with the laser will be useful for creating donor corneal tissue for deeper anterior lamellar keratoplasty or endothelial keratoplasty surgery or both from a single donor cornea.Objetivo: Avaliar a eficácia e segurança de um laser de femtossegundo de baixa energia e alta taxa de repetição para confecção de lamelas corneanas doadoras anteriores profundas. Métodos: Este é um estudo prospectivo de investigação laboratorial. Vinte e cinco botões corneanos foram cortados com laser de femtossegundo para criar lamelas corneanas doadoras anteriores profundas (diâmetro, 10mm; espessura, 500µm). O corte a laser foi realizado com femtosecond laser LDV® na câmara anterior artificial da Ziemer®. Para obter-se uma melhor borda, as lamelas foram trepanadas com um trépano de 8mm da Katena®. A paquimetria corneana central e as lamelas anteriores foram aferidas utilizando o paquímetro Mitutoyo®, com acurácia de 0.001mm. Resultados: A paquimetria central das 25 córneas variou de 500 a 705µm (média de 584 ± 51µm). A espessura das lamelas anteriores variou de 420 a 480µm (media de 455 ± 12.7µm). O diâmetro das lamelas corneanas doadoras foi 7.90 ± 0,1mm, sendo todos os cortes redondos. As interfaces lamelares apresentaram-se regular ao microscópio cirúrgico. Não houve casos de adesão interlamelar. Conclusão: O laser de femtossegundo LDV® mostrou-se seguro e eficaz para confeccionar lamelas corneanas doadoras a partir de botões córneo-esclerais. Mínima variação na espessura das lamelas doadoras confeccionadas com o laser será útil para criação de tecidos corneanos doadores para ceratoplastia lamelar anterior profunda ou ceratoplastia endoteliais, ou ambas, a partir de uma só córnea.Universidade de São Paulo Department of OphthalmologyUniversidade Federal de São Paulo (UNIFESP) Department of OphthalmologyHarvard Medical School Department of OphthalmologyUNIFESP, Department of OphthalmologySciELOSociedade Brasileira de OftalmologiaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Harvard Medical School Department of OphthalmologyVictor, GustavoNosé, Walton [UNIFESP]Faria e Sousa, Sidney Julio dePineda, RobertoAlves, Milton Ruiz2015-06-14T13:47:04Z2015-06-14T13:47:04Z2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion71-74application/pdfhttp://dx.doi.org/10.5935/0034-7280.20140017Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 73, n. 2, p. 71-74, 2014.10.5935/0034-7280.20140017S0034-72802014000200071.pdf0034-7280S0034-72802014000200071http://repositorio.unifesp.br/handle/11600/8360engRevista Brasileira de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T13:30:19Zoai:repositorio.unifesp.br/:11600/8360Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T13:30:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
Laser de femtossegundo de baixa energia e alta frequência na confecção de lamelas corneanas doadoras
title Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
spellingShingle Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
Victor, Gustavo
Cornea
Corneal transplantation
Corneal pachymetry
Endothelium
Lasers
Córnea
Transplante de córnea
Paquimetria corneana
Lasers
Endotélio
title_short Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
title_full Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
title_fullStr Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
title_full_unstemmed Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
title_sort Analysis of low-energy and high-frequency femtosecond laser for the construction of deep anterior donor corneal lamellae
author Victor, Gustavo
author_facet Victor, Gustavo
Nosé, Walton [UNIFESP]
Faria e Sousa, Sidney Julio de
Pineda, Roberto
Alves, Milton Ruiz
author_role author
author2 Nosé, Walton [UNIFESP]
Faria e Sousa, Sidney Julio de
Pineda, Roberto
Alves, Milton Ruiz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Harvard Medical School Department of Ophthalmology
dc.contributor.author.fl_str_mv Victor, Gustavo
Nosé, Walton [UNIFESP]
Faria e Sousa, Sidney Julio de
Pineda, Roberto
Alves, Milton Ruiz
dc.subject.por.fl_str_mv Cornea
Corneal transplantation
Corneal pachymetry
Endothelium
Lasers
Córnea
Transplante de córnea
Paquimetria corneana
Lasers
Endotélio
topic Cornea
Corneal transplantation
Corneal pachymetry
Endothelium
Lasers
Córnea
Transplante de córnea
Paquimetria corneana
Lasers
Endotélio
description Purpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500µm). The laser cuts were made using an LDV® femtosecond laser in a Ziemer® anterior chamber. To obtain a better edge, the lamellae were trephined with an 8mm trephine (Katena®). The central corneal thickness and the anterior lamellae were measured using a Mitutoyo® thickness gauge with an accuracy of 0.001mm. Results: The central thickness of the 25 corneas ranged from 500 to 705µm (mean, 584 ± 51µm). The thickness of the anterior lamellae ranged from 420 to 480µm (mean, 455 ± 12.7µm). The anterior lamellae diameters were 7.90 ± 0.1mm, and all laser cuts were round. The lamellar interfaces appeared regular by surgical microscopy. There were no cases of inter-lamellar adhesion. Conclusion: The LDV® femtosecond laser appears to be a safe and reliable instrument for cutting deep anterior lamellae from donor corneoscleral buttons. Minimal variation in donor lamellar depth with the laser will be useful for creating donor corneal tissue for deeper anterior lamellar keratoplasty or endothelial keratoplasty surgery or both from a single donor cornea.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
2015-06-14T13:47:04Z
2015-06-14T13:47:04Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/0034-7280.20140017
Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 73, n. 2, p. 71-74, 2014.
10.5935/0034-7280.20140017
S0034-72802014000200071.pdf
0034-7280
S0034-72802014000200071
http://repositorio.unifesp.br/handle/11600/8360
url http://dx.doi.org/10.5935/0034-7280.20140017
http://repositorio.unifesp.br/handle/11600/8360
identifier_str_mv Revista Brasileira de Oftalmologia. Sociedade Brasileira de Oftalmologia, v. 73, n. 2, p. 71-74, 2014.
10.5935/0034-7280.20140017
S0034-72802014000200071.pdf
0034-7280
S0034-72802014000200071
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 71-74
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
publisher.none.fl_str_mv Sociedade Brasileira de Oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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