Detection of mycobacteria in coalho cheese sold in Northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Medeiros, Giovanni Brito
Data de Publicação: 2021
Outros Autores: Rodrigues, Onaldo Guedes, Bezerra Neto, Pirajá Saraiva, Medeiros, Rosália Severo de, Sarti, Elaine Cristina Fernandes Baez, Araújo, Flábio Ribeiro de, Azevedo, Sérgio Santos de, Higino, Severino Silvano dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459
Resumo: The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).
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spelling Detection of mycobacteria in coalho cheese sold in Northeastern BrazilDetecção de micobactérias em queijo de coalho comercializado no Nordeste do BrasilCoalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilumNested-PCR.Queijo de coalhoMycobacterium lehmaniiMycobacterium ssp.Mycobacterium rutilumNested-PCR.The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).O presente estudo teve como objetivo detectar e identificar Mycobaterium spp. em 50 amostras de queijo de coalho comercializados na região Nordeste do Brasil. Das 50 amostras analisadas, 35 foram produzidas pelo processo artesanal, com leite cru e 15 provenientes de estabelecimentos industrializados que realizam a pasteurização do leite. Nested-PCR convencional e em tempo real para o gene rv2807 do Complexo M. tuberculosis foi realizada diretamente das cinquenta amostras analisadas. Paralelamente, as amostras de queijo de coalho foram cultivadas em meio de Stonebrink e dos isolados bacterianos foi realizada PCR convencional com iniciadores que flanqueiam a região de diferenciação 4 (RD4), específica de M. bovis, mb400F. Os isolados bacterianos negativos na PCR para RD4 foram submetidos à PCR para hsp65 de Mycobacterium spp., com posterior sequenciamento do DNA. As culturas mostraram-se negativas para o Complexo M. tuberculosis, porém duas amostras oriundas do processo artesanal (com leite cru) (4%) apresentaram identidade com hsp65 de Mycobacterium lehmanii (ID da sequência: KY933786.1, identidades: 312/363 [86%]); e Mycobacterium rutilum (ID da sequência: LT629971.1, identidades: 331/371 [89%]), apontando-se como indicativos de contaminação ambiental. As micobactérias-não-tuberculosas (MNT) são microrganismos emergentes e de natureza ubíqua. Devido a essas características merecem maior atenção na cadeia produtiva de queijos, tanto nas boas práticas agropecuárias (BPAs) quanto nas boas práticas de fabricação de alimentos (BPFs).UEL2021-01-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa de CampoAvaliado por paresPesquisa de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4045910.5433/1679-0359.2021v42n1p439Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 439-446Semina: Ciências Agrárias; v. 42 n. 1 (2021); 439-4461679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459/28523Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMedeiros, Giovanni BritoRodrigues, Onaldo GuedesBezerra Neto, Pirajá SaraivaMedeiros, Rosália Severo deSarti, Elaine Cristina Fernandes BaezAraújo, Flábio Ribeiro deAzevedo, Sérgio Santos deHigino, Severino Silvano dos Santos2022-10-04T15:58:19Zoai:ojs.pkp.sfu.ca:article/40459Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T15:58:19Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
Detecção de micobactérias em queijo de coalho comercializado no Nordeste do Brasil
title Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
spellingShingle Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
Medeiros, Giovanni Brito
Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum
Nested-PCR.
Queijo de coalho
Mycobacterium lehmanii
Mycobacterium ssp.
Mycobacterium rutilum
Nested-PCR.
title_short Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
title_full Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
title_fullStr Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
title_full_unstemmed Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
title_sort Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
author Medeiros, Giovanni Brito
author_facet Medeiros, Giovanni Brito
Rodrigues, Onaldo Guedes
Bezerra Neto, Pirajá Saraiva
Medeiros, Rosália Severo de
Sarti, Elaine Cristina Fernandes Baez
Araújo, Flábio Ribeiro de
Azevedo, Sérgio Santos de
Higino, Severino Silvano dos Santos
author_role author
author2 Rodrigues, Onaldo Guedes
Bezerra Neto, Pirajá Saraiva
Medeiros, Rosália Severo de
Sarti, Elaine Cristina Fernandes Baez
Araújo, Flábio Ribeiro de
Azevedo, Sérgio Santos de
Higino, Severino Silvano dos Santos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Medeiros, Giovanni Brito
Rodrigues, Onaldo Guedes
Bezerra Neto, Pirajá Saraiva
Medeiros, Rosália Severo de
Sarti, Elaine Cristina Fernandes Baez
Araújo, Flábio Ribeiro de
Azevedo, Sérgio Santos de
Higino, Severino Silvano dos Santos
dc.subject.por.fl_str_mv Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum
Nested-PCR.
Queijo de coalho
Mycobacterium lehmanii
Mycobacterium ssp.
Mycobacterium rutilum
Nested-PCR.
topic Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum
Nested-PCR.
Queijo de coalho
Mycobacterium lehmanii
Mycobacterium ssp.
Mycobacterium rutilum
Nested-PCR.
description The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).
publishDate 2021
dc.date.none.fl_str_mv 2021-01-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa de Campo
Avaliado por pares
Pesquisa de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459
10.5433/1679-0359.2021v42n1p439
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459
identifier_str_mv 10.5433/1679-0359.2021v42n1p439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459/28523
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 439-446
Semina: Ciências Agrárias; v. 42 n. 1 (2021); 439-446
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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