Detection of mycobacteria in coalho cheese sold in Northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459 |
Resumo: | The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP). |
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Semina. Ciências Agrárias (Online) |
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Detection of mycobacteria in coalho cheese sold in Northeastern BrazilDetecção de micobactérias em queijo de coalho comercializado no Nordeste do BrasilCoalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilumNested-PCR.Queijo de coalhoMycobacterium lehmaniiMycobacterium ssp.Mycobacterium rutilumNested-PCR.The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).O presente estudo teve como objetivo detectar e identificar Mycobaterium spp. em 50 amostras de queijo de coalho comercializados na região Nordeste do Brasil. Das 50 amostras analisadas, 35 foram produzidas pelo processo artesanal, com leite cru e 15 provenientes de estabelecimentos industrializados que realizam a pasteurização do leite. Nested-PCR convencional e em tempo real para o gene rv2807 do Complexo M. tuberculosis foi realizada diretamente das cinquenta amostras analisadas. Paralelamente, as amostras de queijo de coalho foram cultivadas em meio de Stonebrink e dos isolados bacterianos foi realizada PCR convencional com iniciadores que flanqueiam a região de diferenciação 4 (RD4), específica de M. bovis, mb400F. Os isolados bacterianos negativos na PCR para RD4 foram submetidos à PCR para hsp65 de Mycobacterium spp., com posterior sequenciamento do DNA. As culturas mostraram-se negativas para o Complexo M. tuberculosis, porém duas amostras oriundas do processo artesanal (com leite cru) (4%) apresentaram identidade com hsp65 de Mycobacterium lehmanii (ID da sequência: KY933786.1, identidades: 312/363 [86%]); e Mycobacterium rutilum (ID da sequência: LT629971.1, identidades: 331/371 [89%]), apontando-se como indicativos de contaminação ambiental. As micobactérias-não-tuberculosas (MNT) são microrganismos emergentes e de natureza ubíqua. Devido a essas características merecem maior atenção na cadeia produtiva de queijos, tanto nas boas práticas agropecuárias (BPAs) quanto nas boas práticas de fabricação de alimentos (BPFs).UEL2021-01-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa de CampoAvaliado por paresPesquisa de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4045910.5433/1679-0359.2021v42n1p439Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 439-446Semina: Ciências Agrárias; v. 42 n. 1 (2021); 439-4461679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459/28523Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMedeiros, Giovanni BritoRodrigues, Onaldo GuedesBezerra Neto, Pirajá SaraivaMedeiros, Rosália Severo deSarti, Elaine Cristina Fernandes BaezAraújo, Flábio Ribeiro deAzevedo, Sérgio Santos deHigino, Severino Silvano dos Santos2022-10-04T15:58:19Zoai:ojs.pkp.sfu.ca:article/40459Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T15:58:19Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil Detecção de micobactérias em queijo de coalho comercializado no Nordeste do Brasil |
title |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
spellingShingle |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil Medeiros, Giovanni Brito Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. Queijo de coalho Mycobacterium lehmanii Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. |
title_short |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
title_full |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
title_fullStr |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
title_full_unstemmed |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
title_sort |
Detection of mycobacteria in coalho cheese sold in Northeastern Brazil |
author |
Medeiros, Giovanni Brito |
author_facet |
Medeiros, Giovanni Brito Rodrigues, Onaldo Guedes Bezerra Neto, Pirajá Saraiva Medeiros, Rosália Severo de Sarti, Elaine Cristina Fernandes Baez Araújo, Flábio Ribeiro de Azevedo, Sérgio Santos de Higino, Severino Silvano dos Santos |
author_role |
author |
author2 |
Rodrigues, Onaldo Guedes Bezerra Neto, Pirajá Saraiva Medeiros, Rosália Severo de Sarti, Elaine Cristina Fernandes Baez Araújo, Flábio Ribeiro de Azevedo, Sérgio Santos de Higino, Severino Silvano dos Santos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros, Giovanni Brito Rodrigues, Onaldo Guedes Bezerra Neto, Pirajá Saraiva Medeiros, Rosália Severo de Sarti, Elaine Cristina Fernandes Baez Araújo, Flábio Ribeiro de Azevedo, Sérgio Santos de Higino, Severino Silvano dos Santos |
dc.subject.por.fl_str_mv |
Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. Queijo de coalho Mycobacterium lehmanii Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. |
topic |
Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. Queijo de coalho Mycobacterium lehmanii Mycobacterium ssp. Mycobacterium rutilum Nested-PCR. |
description |
The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa de Campo Avaliado por pares Pesquisa de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459 10.5433/1679-0359.2021v42n1p439 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459 |
identifier_str_mv |
10.5433/1679-0359.2021v42n1p439 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40459/28523 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 439-446 Semina: Ciências Agrárias; v. 42 n. 1 (2021); 439-446 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306083322298368 |