Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Semina. Ciências Agrárias (Online) |
Download full: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15950 |
Summary: | Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil. |
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Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinosLongitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattleAnaplasma marginaleBlood smearELISAPCR.Medicina VeterináriaAnaplasma marginaleBlood smearELISAPCR.Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil.Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil.UEL2014-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa observacionalapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1595010.5433/1679-0359.2014v35n4Suplp2491Semina: Ciências Agrárias; Vol. 35 No. 4Supl (2014); 2491-2500Semina: Ciências Agrárias; v. 35 n. 4Supl (2014); 2491-25001679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15950/pdf_430Copyright (c) 2014 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Jenevaldo Barbosa daCastro, Gustavo Nunes de SantanaFonseca, Adivaldo Henrique2023-01-16T13:59:36Zoai:ojs.pkp.sfu.ca:article/15950Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-16T13:59:36Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle |
title |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
spellingShingle |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos Silva, Jenevaldo Barbosa da Anaplasma marginale Blood smear ELISA PCR. Medicina Veterinária Anaplasma marginale Blood smear ELISA PCR. |
title_short |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
title_full |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
title_fullStr |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
title_full_unstemmed |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
title_sort |
Estudo longitudinal dos fatores de risco para anaplasmose e transmissão transplacentária em rebanhos bovinos |
author |
Silva, Jenevaldo Barbosa da |
author_facet |
Silva, Jenevaldo Barbosa da Castro, Gustavo Nunes de Santana Fonseca, Adivaldo Henrique |
author_role |
author |
author2 |
Castro, Gustavo Nunes de Santana Fonseca, Adivaldo Henrique |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva, Jenevaldo Barbosa da Castro, Gustavo Nunes de Santana Fonseca, Adivaldo Henrique |
dc.subject.por.fl_str_mv |
Anaplasma marginale Blood smear ELISA PCR. Medicina Veterinária Anaplasma marginale Blood smear ELISA PCR. |
topic |
Anaplasma marginale Blood smear ELISA PCR. Medicina Veterinária Anaplasma marginale Blood smear ELISA PCR. |
description |
Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa observacional |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15950 10.5433/1679-0359.2014v35n4Suplp2491 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15950 |
identifier_str_mv |
10.5433/1679-0359.2014v35n4Suplp2491 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15950/pdf_430 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2014 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2014 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 35 No. 4Supl (2014); 2491-2500 Semina: Ciências Agrárias; v. 35 n. 4Supl (2014); 2491-2500 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306070389161984 |