Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system

Detalhes bibliográficos
Autor(a) principal: Oliveira, Camila Costa de
Data de Publicação: 2021
Outros Autores: Bertholoto, Danielli Monsores, Nascimento, Elisamara Caldeira do, Zonta, Everaldo, Coelho, Shana de Mattos de Oliveira, Souza, Miliane Moreira Soares de, Melo, Paulo Regis Bandeira, Coelho, Irene da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40859
Resumo: Animal waste is widely used in organic production systems. However, these residues can increase antimicrobial determinants in the soil. In this perspective, this study was developed to evaluate the presence of sulfonamide resistance genes in soils from an organic production system that received animal waste as organic fertilizer. Soil samples were collected from four properties with different management practices to increase soil fertility. Three properties use the animal waste from the conventional system and the other use plant residues as soil cover and a legal reserve. The extraction of total DNA from soil was carried out followed by the amplification of genes encoding sulfonamide resistance (sul1 and sul2) by the PCR (polymerase chain reaction) technique. The sul1 and sul2 genes were detected only in soils treated with animal waste. The genes were not detected in soils from the legal reserve and the property that used plant residues as soil cover. These results indicate that the use of animal waste as agricultural fertilizer can increase genes for resistance to antimicrobials in the soil and the composting process may not be enough to eliminate them. This information reiterates the need to implement standards that establish quality parameters for animal waste, considering resistance to antimicrobials, as well as the development of management strategies that reduce the risk of spreading resistance to antimicrobials when these residues are applied to soils.
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spelling Sulfonamide resistance genes in soils treated with waste from animal production in an organic production systemGenes de resistência a sulfonamida em solos tratados com resíduos da produção animal em sistema orgânico de produçãoAnimal wasteAntimicrobial resistanceBovine manurePoultry litter.Resíduos animaisResistência a antimicrobianosEsterco bovinoEsterco de galinha.Animal waste is widely used in organic production systems. However, these residues can increase antimicrobial determinants in the soil. In this perspective, this study was developed to evaluate the presence of sulfonamide resistance genes in soils from an organic production system that received animal waste as organic fertilizer. Soil samples were collected from four properties with different management practices to increase soil fertility. Three properties use the animal waste from the conventional system and the other use plant residues as soil cover and a legal reserve. The extraction of total DNA from soil was carried out followed by the amplification of genes encoding sulfonamide resistance (sul1 and sul2) by the PCR (polymerase chain reaction) technique. The sul1 and sul2 genes were detected only in soils treated with animal waste. The genes were not detected in soils from the legal reserve and the property that used plant residues as soil cover. These results indicate that the use of animal waste as agricultural fertilizer can increase genes for resistance to antimicrobials in the soil and the composting process may not be enough to eliminate them. This information reiterates the need to implement standards that establish quality parameters for animal waste, considering resistance to antimicrobials, as well as the development of management strategies that reduce the risk of spreading resistance to antimicrobials when these residues are applied to soils.Resíduos animais são amplamente utilizados em sistemas de produção orgânicos. No entanto, esses resíduos podem incrementar determinantes antimicrobianos no solo. Nesta perspectiva, este estudo foi desenvolvido para avaliar a presença de genes de resistência à sulfonamida em solos do sistema de produção orgânico que receberam resíduos de animais como fertilizante orgânico. Amostras de solo foram coletadas de quatro propriedades com diferentes formas de manejo para aumentar a fertilidade do solo. Três utilizaram resíduos animais do sistema convencional; uma utilizou resíduos vegetais como cobertura do solo, além de uma reserva legal. Foi realizada a extração do DNA total do solo, seguida da amplificação dos genes que codificam resistência sulfonamida (sul1 e sul2) pela técnica de PCR (Polymerase Chain Reaction). Os genes sul1 e sul2 foram detectados apenas nos solos tratados com dejetos de animais. Não foram detectados nos solos da reserva legal e das propriedades que utilizavam resíduos vegetais como cobertura do solo. Esses resultados indicam que o uso de resíduos animais como fertilizante agrícola pode incrementar genes de resistência aos antimicrobianos no solo e que o processo de compostagem pode não ser suficiente para eliminá-los. Essas informações reiteram a necessidade de implementar padrões que estabeleçam parâmetros de qualidade para os resíduos animais, levando em conta a resistência aos antimicrobianos, bem como o desenvolvimento de estratégias de manejo que reduzam o risco de disseminação da resistência aos antimicrobianos quando esses resíduos são aplicados no solo.UEL2021-04-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMétodo hipotético-dedutivoAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4085910.5433/1679-0359.2021v42n3Supl1p2031Semina: Ciências Agrárias; Vol. 42 No. 3Supl1 (2021); 2031-2040Semina: Ciências Agrárias; v. 42 n. 3Supl1 (2021); 2031-20401679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40859/29473Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessOliveira, Camila Costa deBertholoto, Danielli MonsoresNascimento, Elisamara Caldeira doZonta, EveraldoCoelho, Shana de Mattos de OliveiraSouza, Miliane Moreira Soares deMelo, Paulo Regis BandeiraCoelho, Irene da Silva2022-09-30T16:53:31Zoai:ojs.pkp.sfu.ca:article/40859Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T16:53:31Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
Genes de resistência a sulfonamida em solos tratados com resíduos da produção animal em sistema orgânico de produção
title Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
spellingShingle Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
Oliveira, Camila Costa de
Animal waste
Antimicrobial resistance
Bovine manure
Poultry litter.
Resíduos animais
Resistência a antimicrobianos
Esterco bovino
Esterco de galinha.
title_short Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
title_full Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
title_fullStr Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
title_full_unstemmed Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
title_sort Sulfonamide resistance genes in soils treated with waste from animal production in an organic production system
author Oliveira, Camila Costa de
author_facet Oliveira, Camila Costa de
Bertholoto, Danielli Monsores
Nascimento, Elisamara Caldeira do
Zonta, Everaldo
Coelho, Shana de Mattos de Oliveira
Souza, Miliane Moreira Soares de
Melo, Paulo Regis Bandeira
Coelho, Irene da Silva
author_role author
author2 Bertholoto, Danielli Monsores
Nascimento, Elisamara Caldeira do
Zonta, Everaldo
Coelho, Shana de Mattos de Oliveira
Souza, Miliane Moreira Soares de
Melo, Paulo Regis Bandeira
Coelho, Irene da Silva
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Camila Costa de
Bertholoto, Danielli Monsores
Nascimento, Elisamara Caldeira do
Zonta, Everaldo
Coelho, Shana de Mattos de Oliveira
Souza, Miliane Moreira Soares de
Melo, Paulo Regis Bandeira
Coelho, Irene da Silva
dc.subject.por.fl_str_mv Animal waste
Antimicrobial resistance
Bovine manure
Poultry litter.
Resíduos animais
Resistência a antimicrobianos
Esterco bovino
Esterco de galinha.
topic Animal waste
Antimicrobial resistance
Bovine manure
Poultry litter.
Resíduos animais
Resistência a antimicrobianos
Esterco bovino
Esterco de galinha.
description Animal waste is widely used in organic production systems. However, these residues can increase antimicrobial determinants in the soil. In this perspective, this study was developed to evaluate the presence of sulfonamide resistance genes in soils from an organic production system that received animal waste as organic fertilizer. Soil samples were collected from four properties with different management practices to increase soil fertility. Three properties use the animal waste from the conventional system and the other use plant residues as soil cover and a legal reserve. The extraction of total DNA from soil was carried out followed by the amplification of genes encoding sulfonamide resistance (sul1 and sul2) by the PCR (polymerase chain reaction) technique. The sul1 and sul2 genes were detected only in soils treated with animal waste. The genes were not detected in soils from the legal reserve and the property that used plant residues as soil cover. These results indicate that the use of animal waste as agricultural fertilizer can increase genes for resistance to antimicrobials in the soil and the composting process may not be enough to eliminate them. This information reiterates the need to implement standards that establish quality parameters for animal waste, considering resistance to antimicrobials, as well as the development of management strategies that reduce the risk of spreading resistance to antimicrobials when these residues are applied to soils.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Método hipotético-dedutivo
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40859
10.5433/1679-0359.2021v42n3Supl1p2031
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40859
identifier_str_mv 10.5433/1679-0359.2021v42n3Supl1p2031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40859/29473
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 3Supl1 (2021); 2031-2040
Semina: Ciências Agrárias; v. 42 n. 3Supl1 (2021); 2031-2040
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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