Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4

Detalhes bibliográficos
Autor(a) principal: Casotti, Josiani Fatima
Data de Publicação: 2007
Outros Autores: Camilios Neto, Doumit, Bruzon, Gilcelene, Machado, Robson Alessandro Mattos, Buzato, João Batista, Celligoi, Maria Antonia Pedrine Colabone
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896
Resumo: Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l).
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spelling Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4Uso de matérias primas da agroindústria – garapa e extrato de levedura – na produção de asparaginase por Zymomonas mobilis CP4Sugar cane juiceZymomonas mobilisAsparaginaseFermentation.GarapaZymomonas mobilisAsparaginaseFermentação.Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l).Garapa e extrato de levedura foram usados na produção de asparaginase por Zymomonas mobilis CP4. Na otimização utilizou metodologia de superfície de resposta com 2 variáveis (extrato de levedura e asparagina) em 3 níveis (1,0; 5,5 e 10,0 g/L) e uma repetição do ponto central. A fermentação em batelada utilizou garapa diluída a 8 % (P/V) de Açúcares Totais e inóculo de Zymomonas mobilis CP4 na concentração de 2 mg/mL. Após a fermentação de 18 horas, a maior produção obtida de asparaginase foi de 9,75 U/L em extrato de levedura em 5,5 g/L e asparagina em 1,0 g/L.UEL2007-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/289610.5433/1679-0359.2007v28n4p653Semina: Ciências Agrárias; Vol. 28 No. 4 (2007); 653-658Semina: Ciências Agrárias; v. 28 n. 4 (2007); 653-6581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896/2455Casotti, Josiani FatimaCamilios Neto, DoumitBruzon, GilceleneMachado, Robson Alessandro MattosBuzato, João BatistaCelligoi, Maria Antonia Pedrine Colaboneinfo:eu-repo/semantics/openAccess2015-11-19T18:38:54Zoai:ojs.pkp.sfu.ca:article/2896Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
Uso de matérias primas da agroindústria – garapa e extrato de levedura – na produção de asparaginase por Zymomonas mobilis CP4
title Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
spellingShingle Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
Casotti, Josiani Fatima
Sugar cane juice
Zymomonas mobilis
Asparaginase
Fermentation.
Garapa
Zymomonas mobilis
Asparaginase
Fermentação.
title_short Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
title_full Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
title_fullStr Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
title_full_unstemmed Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
title_sort Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
author Casotti, Josiani Fatima
author_facet Casotti, Josiani Fatima
Camilios Neto, Doumit
Bruzon, Gilcelene
Machado, Robson Alessandro Mattos
Buzato, João Batista
Celligoi, Maria Antonia Pedrine Colabone
author_role author
author2 Camilios Neto, Doumit
Bruzon, Gilcelene
Machado, Robson Alessandro Mattos
Buzato, João Batista
Celligoi, Maria Antonia Pedrine Colabone
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Casotti, Josiani Fatima
Camilios Neto, Doumit
Bruzon, Gilcelene
Machado, Robson Alessandro Mattos
Buzato, João Batista
Celligoi, Maria Antonia Pedrine Colabone
dc.subject.por.fl_str_mv Sugar cane juice
Zymomonas mobilis
Asparaginase
Fermentation.
Garapa
Zymomonas mobilis
Asparaginase
Fermentação.
topic Sugar cane juice
Zymomonas mobilis
Asparaginase
Fermentation.
Garapa
Zymomonas mobilis
Asparaginase
Fermentação.
description Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l).
publishDate 2007
dc.date.none.fl_str_mv 2007-08-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896
10.5433/1679-0359.2007v28n4p653
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896
identifier_str_mv 10.5433/1679-0359.2007v28n4p653
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896/2455
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 28 No. 4 (2007); 653-658
Semina: Ciências Agrárias; v. 28 n. 4 (2007); 653-658
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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