Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896 |
Resumo: | Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l). |
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oai:ojs.pkp.sfu.ca:article/2896 |
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Semina. Ciências Agrárias (Online) |
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Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4Uso de matérias primas da agroindústria – garapa e extrato de levedura – na produção de asparaginase por Zymomonas mobilis CP4Sugar cane juiceZymomonas mobilisAsparaginaseFermentation.GarapaZymomonas mobilisAsparaginaseFermentação.Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l).Garapa e extrato de levedura foram usados na produção de asparaginase por Zymomonas mobilis CP4. Na otimização utilizou metodologia de superfície de resposta com 2 variáveis (extrato de levedura e asparagina) em 3 níveis (1,0; 5,5 e 10,0 g/L) e uma repetição do ponto central. A fermentação em batelada utilizou garapa diluída a 8 % (P/V) de Açúcares Totais e inóculo de Zymomonas mobilis CP4 na concentração de 2 mg/mL. Após a fermentação de 18 horas, a maior produção obtida de asparaginase foi de 9,75 U/L em extrato de levedura em 5,5 g/L e asparagina em 1,0 g/L.UEL2007-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/289610.5433/1679-0359.2007v28n4p653Semina: Ciências Agrárias; Vol. 28 No. 4 (2007); 653-658Semina: Ciências Agrárias; v. 28 n. 4 (2007); 653-6581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896/2455Casotti, Josiani FatimaCamilios Neto, DoumitBruzon, GilceleneMachado, Robson Alessandro MattosBuzato, João BatistaCelligoi, Maria Antonia Pedrine Colaboneinfo:eu-repo/semantics/openAccess2015-11-19T18:38:54Zoai:ojs.pkp.sfu.ca:article/2896Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 Uso de matérias primas da agroindústria – garapa e extrato de levedura – na produção de asparaginase por Zymomonas mobilis CP4 |
title |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
spellingShingle |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 Casotti, Josiani Fatima Sugar cane juice Zymomonas mobilis Asparaginase Fermentation. Garapa Zymomonas mobilis Asparaginase Fermentação. |
title_short |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
title_full |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
title_fullStr |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
title_full_unstemmed |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
title_sort |
Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4 |
author |
Casotti, Josiani Fatima |
author_facet |
Casotti, Josiani Fatima Camilios Neto, Doumit Bruzon, Gilcelene Machado, Robson Alessandro Mattos Buzato, João Batista Celligoi, Maria Antonia Pedrine Colabone |
author_role |
author |
author2 |
Camilios Neto, Doumit Bruzon, Gilcelene Machado, Robson Alessandro Mattos Buzato, João Batista Celligoi, Maria Antonia Pedrine Colabone |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Casotti, Josiani Fatima Camilios Neto, Doumit Bruzon, Gilcelene Machado, Robson Alessandro Mattos Buzato, João Batista Celligoi, Maria Antonia Pedrine Colabone |
dc.subject.por.fl_str_mv |
Sugar cane juice Zymomonas mobilis Asparaginase Fermentation. Garapa Zymomonas mobilis Asparaginase Fermentação. |
topic |
Sugar cane juice Zymomonas mobilis Asparaginase Fermentation. Garapa Zymomonas mobilis Asparaginase Fermentação. |
description |
Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l). |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896 10.5433/1679-0359.2007v28n4p653 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896 |
identifier_str_mv |
10.5433/1679-0359.2007v28n4p653 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2896/2455 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 28 No. 4 (2007); 653-658 Semina: Ciências Agrárias; v. 28 n. 4 (2007); 653-658 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306059177787392 |