Virulence of Escherichia coli O139:K82

Detalhes bibliográficos
Autor(a) principal: Brito, Benito Guimarães de
Data de Publicação: 2004
Outros Autores: Tagliari, Kelly Cristina, Piffer, Itamar Antônio, Leite, Domingos da Silva, Silva, Ari Bernardes da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2030
Resumo: In order to evaluate the pathogenicity and antimicrobial resistance pattern of BK 125 Escherichia coli strain, several tests were assessed: serotype, slide agglutination for detection of the fimbriae F18, presence of the gene Stx2e, verotoxin production, hemolytic activity, pathogenicity assessement using piglet inoculation and antimicrobial resistance to drugs. The strain BK125 showed the following profile: F18+, Stx2e+, Hly+, resistance to streptomicin, tetraciclin, sulfonamides. It produced clinical disease and death of infected piglets. Moreover, it was possible to recover the BK125 strain from diarrheic feces and from the gut contents of the piglet, with high rate of recovery of colonies expressing fimbriae F18. The present results suggest that the E. coli BK125 (O139:K82) strain could produce virulence factors and experimentally reproduce oedema disease in pigs.
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spelling Virulence of Escherichia coli O139:K82Virulência de Escherichia coli O139:K82DiarrhoeaPigletsOedema diseaseVirulence factors.DiarréiaLeitõesDoença do edemaFatores de virulência.In order to evaluate the pathogenicity and antimicrobial resistance pattern of BK 125 Escherichia coli strain, several tests were assessed: serotype, slide agglutination for detection of the fimbriae F18, presence of the gene Stx2e, verotoxin production, hemolytic activity, pathogenicity assessement using piglet inoculation and antimicrobial resistance to drugs. The strain BK125 showed the following profile: F18+, Stx2e+, Hly+, resistance to streptomicin, tetraciclin, sulfonamides. It produced clinical disease and death of infected piglets. Moreover, it was possible to recover the BK125 strain from diarrheic feces and from the gut contents of the piglet, with high rate of recovery of colonies expressing fimbriae F18. The present results suggest that the E. coli BK125 (O139:K82) strain could produce virulence factors and experimentally reproduce oedema disease in pigs.Com o objetivo de identificar a patogenicidade e resistência a antimicrobianos da cepa de Escherichia coli BK125, foram utilizados os seguintes testes: sorotipagem, aglutinação em lâmina para detecção da fímbria F18, PCR para verificar a presença do gene Stx2e e teste de citoxicidade para avaliar a expressão da verotoxina, ensaio para detecção de hemolisinas, patogenicidade em leitões e antibiograma. A cepa BK125 apresentou o seguinte perfil: F18+, Stx2e+, Hly+, resistente a estreptomicina, tetraciclina, sulfonamida e foi capaz de provocar a doença clínica e morte em leitões inoculados. Também foi possível o resgate dessa cepa de fezes diarréicas e do conteúdo intestinal dos leitões revelando assim, alto índice de recuperação de colônias inoculadas. Os resultados permitem concluir que a E. coli BK125 (O139:K82) é produtora de fatores de virulência e reproduz experimentalmente a doença do edema em suínos.UEL2004-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/203010.5433/1679-0359.2001v22n2p119Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 119-122Semina: Ciências Agrárias; v. 22 n. 2 (2001); 119-1221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2030/1748Brito, Benito Guimarães deTagliari, Kelly CristinaPiffer, Itamar AntônioLeite, Domingos da SilvaSilva, Ari Bernardes dainfo:eu-repo/semantics/openAccess2009-05-18T13:38:37Zoai:ojs.pkp.sfu.ca:article/2030Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-05-18T13:38:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Virulence of Escherichia coli O139:K82
Virulência de Escherichia coli O139:K82
title Virulence of Escherichia coli O139:K82
spellingShingle Virulence of Escherichia coli O139:K82
Brito, Benito Guimarães de
Diarrhoea
Piglets
Oedema disease
Virulence factors.
Diarréia
Leitões
Doença do edema
Fatores de virulência.
title_short Virulence of Escherichia coli O139:K82
title_full Virulence of Escherichia coli O139:K82
title_fullStr Virulence of Escherichia coli O139:K82
title_full_unstemmed Virulence of Escherichia coli O139:K82
title_sort Virulence of Escherichia coli O139:K82
author Brito, Benito Guimarães de
author_facet Brito, Benito Guimarães de
Tagliari, Kelly Cristina
Piffer, Itamar Antônio
Leite, Domingos da Silva
Silva, Ari Bernardes da
author_role author
author2 Tagliari, Kelly Cristina
Piffer, Itamar Antônio
Leite, Domingos da Silva
Silva, Ari Bernardes da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Brito, Benito Guimarães de
Tagliari, Kelly Cristina
Piffer, Itamar Antônio
Leite, Domingos da Silva
Silva, Ari Bernardes da
dc.subject.por.fl_str_mv Diarrhoea
Piglets
Oedema disease
Virulence factors.
Diarréia
Leitões
Doença do edema
Fatores de virulência.
topic Diarrhoea
Piglets
Oedema disease
Virulence factors.
Diarréia
Leitões
Doença do edema
Fatores de virulência.
description In order to evaluate the pathogenicity and antimicrobial resistance pattern of BK 125 Escherichia coli strain, several tests were assessed: serotype, slide agglutination for detection of the fimbriae F18, presence of the gene Stx2e, verotoxin production, hemolytic activity, pathogenicity assessement using piglet inoculation and antimicrobial resistance to drugs. The strain BK125 showed the following profile: F18+, Stx2e+, Hly+, resistance to streptomicin, tetraciclin, sulfonamides. It produced clinical disease and death of infected piglets. Moreover, it was possible to recover the BK125 strain from diarrheic feces and from the gut contents of the piglet, with high rate of recovery of colonies expressing fimbriae F18. The present results suggest that the E. coli BK125 (O139:K82) strain could produce virulence factors and experimentally reproduce oedema disease in pigs.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2030
10.5433/1679-0359.2001v22n2p119
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2030
identifier_str_mv 10.5433/1679-0359.2001v22n2p119
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2030/1748
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 119-122
Semina: Ciências Agrárias; v. 22 n. 2 (2001); 119-122
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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