Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil

Detalhes bibliográficos
Autor(a) principal: Cordeiro, Matheus Dias
Data de Publicação: 2022
Outros Autores: Araújo, Izabela Mesquita, Silva, Claúdia Bezerra da, Guterres, Alexandro, Fonseca, Adivaldo Henrique da, Baêta, Bruna de Azevedo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45940
Resumo: The present study aimed to use polymerase chain reaction (PCR) to detect species of the order Piroplasmida, such as Anaplasma spp., Borrelia spp., and Ehrlichia spp., circulating in the blood of Didelphis aurita in a peridomiciliary environment. Blood samples collected from big-eared opossum (Didelphis aurita) were screened for hemoparasites using PCR. The extracted DNA was tested for tick-borne hemoparasites. We were unable to detect hemoparasites, such as Ehrlichia spp., Babesia spp., Anaplasma spp., and Borrelia spp. Theileria DNA was detected in only one sample screened using PCR for an approximately 650-base pair fragment of the 18S rRNA gene. Sequencing and BLAST analysis of a subset of the PCR amplicons revealed 97% (535/553 bp) identity with Theileria bicornis. The detection of Theileria sp. in D. aurita challenges us to pursue more in-depth studies of marsupial piroplasmosids and to evaluate the morphological aspects of the findings and their possible involvement in zoonoses.
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spelling Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, BrazilHemoparasitos em Didelphis aurita capturados em Seropédica, Estado do Rio de Janeiro, BrasilMarsupialTheileriaTicksFleasVector-borne.MarsupialTheileriaCarrapatosPulgasVetores.The present study aimed to use polymerase chain reaction (PCR) to detect species of the order Piroplasmida, such as Anaplasma spp., Borrelia spp., and Ehrlichia spp., circulating in the blood of Didelphis aurita in a peridomiciliary environment. Blood samples collected from big-eared opossum (Didelphis aurita) were screened for hemoparasites using PCR. The extracted DNA was tested for tick-borne hemoparasites. We were unable to detect hemoparasites, such as Ehrlichia spp., Babesia spp., Anaplasma spp., and Borrelia spp. Theileria DNA was detected in only one sample screened using PCR for an approximately 650-base pair fragment of the 18S rRNA gene. Sequencing and BLAST analysis of a subset of the PCR amplicons revealed 97% (535/553 bp) identity with Theileria bicornis. The detection of Theileria sp. in D. aurita challenges us to pursue more in-depth studies of marsupial piroplasmosids and to evaluate the morphological aspects of the findings and their possible involvement in zoonoses.O objetivo do presente estudo foi utilizar a reação em cadeia da polimerase (PCR) para detectar espécies da ordem Piroplasmida, como Anaplasma spp., Borrelia spp. e Ehrlichia spp., circulando no sangue de Didelphis aurita em ambiente peridomiciliar. Amostras de sangue coletadas de gambá da orelha preta (Didelphis aurita) foram triadas para hemoparasitas por meio da PCR. O DNA extraído foi testado para alguns hemoparasitas transmitidos por carrapatos. Não conseguimos detectar hemoparasitos, como Ehrlichia spp., Babesia spp., Anaplasma spp. e Borrelia spp. O DNA de Theileria foi detectado apenas em uma amostra. Um fragmento de ~650 pares de bases do gene 18S rRNA foi sequenciado. e a análise pelo BLAST (um subconjunto de amplicons de PCR) revelou uma identidade de 97% (535/553 pb) com Theileria bicornis. A detecção de Theileria sp. in D. aurita nos desafia a aprofundar estudos sobre piroplasmosídeos marsupiais e avaliar os aspectos morfológicos dos achados e seu possível envolvimento com zoonoses.UEL2022-11-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPolymerase Chain Reaccionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4594010.5433/1679-0359.2022v43n6p2403Semina: Ciências Agrárias; Vol. 43 No. 6 (2022); 2403-2414Semina: Ciências Agrárias; v. 43 n. 6 (2022); 2403-24141679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45940/48047Copyright (c) 2022 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCordeiro, Matheus DiasAraújo, Izabela MesquitaSilva, Claúdia Bezerra daGuterres, AlexandroFonseca, Adivaldo Henrique daBaêta, Bruna de Azevedo2023-01-31T12:58:04Zoai:ojs.pkp.sfu.ca:article/45940Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-31T12:58:04Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
Hemoparasitos em Didelphis aurita capturados em Seropédica, Estado do Rio de Janeiro, Brasil
title Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
spellingShingle Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
Cordeiro, Matheus Dias
Marsupial
Theileria
Ticks
Fleas
Vector-borne.
Marsupial
Theileria
Carrapatos
Pulgas
Vetores.
title_short Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
title_full Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
title_fullStr Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
title_full_unstemmed Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
title_sort Hemoparasites in Didelphis aurita from Seropédica, Rio de Janeiro State, Brazil
author Cordeiro, Matheus Dias
author_facet Cordeiro, Matheus Dias
Araújo, Izabela Mesquita
Silva, Claúdia Bezerra da
Guterres, Alexandro
Fonseca, Adivaldo Henrique da
Baêta, Bruna de Azevedo
author_role author
author2 Araújo, Izabela Mesquita
Silva, Claúdia Bezerra da
Guterres, Alexandro
Fonseca, Adivaldo Henrique da
Baêta, Bruna de Azevedo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cordeiro, Matheus Dias
Araújo, Izabela Mesquita
Silva, Claúdia Bezerra da
Guterres, Alexandro
Fonseca, Adivaldo Henrique da
Baêta, Bruna de Azevedo
dc.subject.por.fl_str_mv Marsupial
Theileria
Ticks
Fleas
Vector-borne.
Marsupial
Theileria
Carrapatos
Pulgas
Vetores.
topic Marsupial
Theileria
Ticks
Fleas
Vector-borne.
Marsupial
Theileria
Carrapatos
Pulgas
Vetores.
description The present study aimed to use polymerase chain reaction (PCR) to detect species of the order Piroplasmida, such as Anaplasma spp., Borrelia spp., and Ehrlichia spp., circulating in the blood of Didelphis aurita in a peridomiciliary environment. Blood samples collected from big-eared opossum (Didelphis aurita) were screened for hemoparasites using PCR. The extracted DNA was tested for tick-borne hemoparasites. We were unable to detect hemoparasites, such as Ehrlichia spp., Babesia spp., Anaplasma spp., and Borrelia spp. Theileria DNA was detected in only one sample screened using PCR for an approximately 650-base pair fragment of the 18S rRNA gene. Sequencing and BLAST analysis of a subset of the PCR amplicons revealed 97% (535/553 bp) identity with Theileria bicornis. The detection of Theileria sp. in D. aurita challenges us to pursue more in-depth studies of marsupial piroplasmosids and to evaluate the morphological aspects of the findings and their possible involvement in zoonoses.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Polymerase Chain Reaccion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45940
10.5433/1679-0359.2022v43n6p2403
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45940
identifier_str_mv 10.5433/1679-0359.2022v43n6p2403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/45940/48047
dc.rights.driver.fl_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 43 No. 6 (2022); 2403-2414
Semina: Ciências Agrárias; v. 43 n. 6 (2022); 2403-2414
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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