In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System

Detalhes bibliográficos
Autor(a) principal: Geron, Luiz Juliano Valério
Data de Publicação: 2017
Outros Autores: Garcia, Jocilaine, Coelho, Kallynka Samara Martins, Aguiar, Sílvia Cristina de, Zanine, Andreson de Moura, Souza, Alexandre Lima de, Carvalho, Joilma Toniolo Honório de, Roberto, Lucas Silva, Sousa Neto, Eurico Lucas de, Ferreira, Daniele de Jesus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26727
Resumo: We evaluated the fractions of protein and carbohydrates in distillers dried grains with solubles (DDGS), corn grain (CG), soybean meal (SM), and corn silage (CS), as well as the in vitro digestibility (IVD) of DDGS, CG, SM, CS, rations containing 0.0, 8.0, 16.0, and 24.0% DDGS, and in vitro fermentation parameters after 24 h of incubation. DDGS were obtained following microbial fermentation for ethanol production from a sugar and alcohol distillery located in the state of Mato Grosso - Brazil. The Cornell Net Carbohydrate and Protein System (CNCPS) was used to determine the protein and carbohydrate fractions of experimental diets. For the in vitro nutrient digestion assay using the experimental foods and experimental diets, two sheep with an average body weight of 26 kg were used as inoculum donors. The in vitro digestibility of food and feed was assayed in three replicates. Fraction A of DDGS CP was 88, 71, and 37% lower in relation to fraction A of SM, CG, and CS, respectively. Fraction B2 of DDGS protein contained 21% CP, which represents 78.84% of DDGS protein in fraction B2, and is higher than the SM, which was 70.44%. The B3 fraction of CP, which is partly released during ruminal fermentation, was 18% lower for SM compared to DDGS, and is expressed in %CP. For carbohydrate fractionation, the DDGS presented 8.64% for the A + B1 fraction on a DM basis, which was 62, 86, and 74% lower compared to those obtained for SM, CG. and CS, respectively. The hemicellulose and cellulose contents of DDGS were higher than those of SM, as verified in fraction B2, with a value of 46.92%, expressed in DM. The in vitro digestibility coefficients (IVDC) of the DDGS nutrients did not differ (p > 0.05) in relation to those of the other experimental foods. The inclusion of DDGS in rations formulated for sheep did not change (p > 0.05) the IVDC of DM, OM, CP. NDF, or ADF, with mean values of 70.93, 70.64, 59.58, 52.83, and 43.40%, respectively. Therefore, DDGS comprise a protein-rich food containing more than 70% CP in fraction B2, with a large amount of carbohydrates bound to the cell wall. In addition, DDGS possess a similar digestibility coefficient to corn grain and soybean meal; however, up to 24% can be included in feed formulations for ruminants without changing the in vitro digestibility coefficient of nutrients.
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spelling In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein SystemDigestibilidade in vitro e caracterização nutricional de grão seco de destilaria com solúveis segundo o sistema CornellRumen degradable proteinRuminal fermentationSheepSoybean meal.Farelo de sojaFermentação ruminalProteína degradável no rúmenOvinos.We evaluated the fractions of protein and carbohydrates in distillers dried grains with solubles (DDGS), corn grain (CG), soybean meal (SM), and corn silage (CS), as well as the in vitro digestibility (IVD) of DDGS, CG, SM, CS, rations containing 0.0, 8.0, 16.0, and 24.0% DDGS, and in vitro fermentation parameters after 24 h of incubation. DDGS were obtained following microbial fermentation for ethanol production from a sugar and alcohol distillery located in the state of Mato Grosso - Brazil. The Cornell Net Carbohydrate and Protein System (CNCPS) was used to determine the protein and carbohydrate fractions of experimental diets. For the in vitro nutrient digestion assay using the experimental foods and experimental diets, two sheep with an average body weight of 26 kg were used as inoculum donors. The in vitro digestibility of food and feed was assayed in three replicates. Fraction A of DDGS CP was 88, 71, and 37% lower in relation to fraction A of SM, CG, and CS, respectively. Fraction B2 of DDGS protein contained 21% CP, which represents 78.84% of DDGS protein in fraction B2, and is higher than the SM, which was 70.44%. The B3 fraction of CP, which is partly released during ruminal fermentation, was 18% lower for SM compared to DDGS, and is expressed in %CP. For carbohydrate fractionation, the DDGS presented 8.64% for the A + B1 fraction on a DM basis, which was 62, 86, and 74% lower compared to those obtained for SM, CG. and CS, respectively. The hemicellulose and cellulose contents of DDGS were higher than those of SM, as verified in fraction B2, with a value of 46.92%, expressed in DM. The in vitro digestibility coefficients (IVDC) of the DDGS nutrients did not differ (p > 0.05) in relation to those of the other experimental foods. The inclusion of DDGS in rations formulated for sheep did not change (p > 0.05) the IVDC of DM, OM, CP. NDF, or ADF, with mean values of 70.93, 70.64, 59.58, 52.83, and 43.40%, respectively. Therefore, DDGS comprise a protein-rich food containing more than 70% CP in fraction B2, with a large amount of carbohydrates bound to the cell wall. In addition, DDGS possess a similar digestibility coefficient to corn grain and soybean meal; however, up to 24% can be included in feed formulations for ruminants without changing the in vitro digestibility coefficient of nutrients.Foram avaliadas as frações da proteína e dos carboidratos de grãos secos de destilaria com solúveis (GSDS), grão de milho (GM), farelo de soja (FS), e silagem de milho (SM), e a digestibilidade in vitro (CDIV) do GSDS, GM, FS, SM e de rações contendo a inclusão de 0,0%; 8,0%; 16,0% e 24,0% de GSDS, bem como os parâmetros de fermentação in vitro após 24 horas de incubação. O GSDS foi obtido após processo de fermentação microbiana para a produção do etanol, de uma destilaria de flex de açúcar e álcool localizada no estado de Mato Grosso - Brasil. Para determinação das frações proteicas e de carboidratos dos alimentos experimentais foi utilizado Cornell Net Carbohydrate and Protein System – CNCPS. Para o ensaio de digestão in vitro dos nutrientes dos alimentos experimentais e das rações experimentais foram utilizados dois ovinos com peso corporal médio de 26 kg, como doadores de inóculo. O ensaio de digestibilidade in vitro dos alimentos e das rações foi conduzido com três repetições de campo. A fração A da PB do GSDS apresentou valores 88%, 71% e 37% menores em relação a fração A do FS, GM e SM, respectivamente. A fração B2 da proteína do GSDS apresentou um teor de 21% da PB, o que representa 78,84% da proteína do GSDS na fração B2, valor superior ao do FS, que foi de 70,44%. A fração B3 da PB, a qual parte escapa da fermentação ruminal foi 18% menor para o FS em relação ao GSDS, expresso em % da PB. Para o fracionamento de carboidratos, o GSDS apresentou um valor para a fração A+ B1 de 8,64% na MS resultado inferior em 62%; 86% e 74% aos obtidos para o FS, GM e SM. O GSDS apresentou teores de hemicelulose e celulose superior ao FS, como verificado na fração B2, com valor de 46,92 % expresso na MS. Os coeficientes de digestibilidade in vitro (CDIV) dos nutrientes do GSDS não diferiu (p > 0,05) em relação aos demais alimentos experimentais. A inclusão do GSDS em rações formuladas para ovinos não alterou (p > 0,05) o CDIV da MS; MO; PB; FDN e FDA, com valores médios de 70,93%; 70,64%; 59,58%; 52,83% e 43,40%, respectivamente. Desta maneira, conclui-se que o grão seco de destilaria com solúveis apresenta-se como alimento proteico com mais de 70% da PB na fração B2 e possui levada quantidade de carboidratos ligados à parede celular. Além disso, o grão seco de destilaria com solúveis apresenta um coeficiente de digestibilidade semelhante ao do grão de milho e ao farelo de soja, contudo, este pode ser incluído em até 24% nas formulações de rações para ruminantes sem alterar o coeficiente de digestibilidade in vitro dos nutrientes.UEL2017-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2672710.5433/1679-0359.2017v38n4p2029Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 2029-2040Semina: Ciências Agrárias; v. 38 n. 4 (2017); 2029-20401679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26727/21379Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGeron, Luiz Juliano ValérioGarcia, JocilaineCoelho, Kallynka Samara MartinsAguiar, Sílvia Cristina deZanine, Andreson de MouraSouza, Alexandre Lima deCarvalho, Joilma Toniolo Honório deRoberto, Lucas SilvaSousa Neto, Eurico Lucas deFerreira, Daniele de Jesus2022-10-24T12:42:08Zoai:ojs.pkp.sfu.ca:article/26727Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T12:42:08Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
Digestibilidade in vitro e caracterização nutricional de grão seco de destilaria com solúveis segundo o sistema Cornell
title In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
spellingShingle In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
Geron, Luiz Juliano Valério
Rumen degradable protein
Ruminal fermentation
Sheep
Soybean meal.
Farelo de soja
Fermentação ruminal
Proteína degradável no rúmen
Ovinos.
title_short In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
title_full In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
title_fullStr In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
title_full_unstemmed In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
title_sort In vitro digestibility and nutritional characterization of distillers dried grains with solubles according to the Cornell Net Carbohydrate and Protein System
author Geron, Luiz Juliano Valério
author_facet Geron, Luiz Juliano Valério
Garcia, Jocilaine
Coelho, Kallynka Samara Martins
Aguiar, Sílvia Cristina de
Zanine, Andreson de Moura
Souza, Alexandre Lima de
Carvalho, Joilma Toniolo Honório de
Roberto, Lucas Silva
Sousa Neto, Eurico Lucas de
Ferreira, Daniele de Jesus
author_role author
author2 Garcia, Jocilaine
Coelho, Kallynka Samara Martins
Aguiar, Sílvia Cristina de
Zanine, Andreson de Moura
Souza, Alexandre Lima de
Carvalho, Joilma Toniolo Honório de
Roberto, Lucas Silva
Sousa Neto, Eurico Lucas de
Ferreira, Daniele de Jesus
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Geron, Luiz Juliano Valério
Garcia, Jocilaine
Coelho, Kallynka Samara Martins
Aguiar, Sílvia Cristina de
Zanine, Andreson de Moura
Souza, Alexandre Lima de
Carvalho, Joilma Toniolo Honório de
Roberto, Lucas Silva
Sousa Neto, Eurico Lucas de
Ferreira, Daniele de Jesus
dc.subject.por.fl_str_mv Rumen degradable protein
Ruminal fermentation
Sheep
Soybean meal.
Farelo de soja
Fermentação ruminal
Proteína degradável no rúmen
Ovinos.
topic Rumen degradable protein
Ruminal fermentation
Sheep
Soybean meal.
Farelo de soja
Fermentação ruminal
Proteína degradável no rúmen
Ovinos.
description We evaluated the fractions of protein and carbohydrates in distillers dried grains with solubles (DDGS), corn grain (CG), soybean meal (SM), and corn silage (CS), as well as the in vitro digestibility (IVD) of DDGS, CG, SM, CS, rations containing 0.0, 8.0, 16.0, and 24.0% DDGS, and in vitro fermentation parameters after 24 h of incubation. DDGS were obtained following microbial fermentation for ethanol production from a sugar and alcohol distillery located in the state of Mato Grosso - Brazil. The Cornell Net Carbohydrate and Protein System (CNCPS) was used to determine the protein and carbohydrate fractions of experimental diets. For the in vitro nutrient digestion assay using the experimental foods and experimental diets, two sheep with an average body weight of 26 kg were used as inoculum donors. The in vitro digestibility of food and feed was assayed in three replicates. Fraction A of DDGS CP was 88, 71, and 37% lower in relation to fraction A of SM, CG, and CS, respectively. Fraction B2 of DDGS protein contained 21% CP, which represents 78.84% of DDGS protein in fraction B2, and is higher than the SM, which was 70.44%. The B3 fraction of CP, which is partly released during ruminal fermentation, was 18% lower for SM compared to DDGS, and is expressed in %CP. For carbohydrate fractionation, the DDGS presented 8.64% for the A + B1 fraction on a DM basis, which was 62, 86, and 74% lower compared to those obtained for SM, CG. and CS, respectively. The hemicellulose and cellulose contents of DDGS were higher than those of SM, as verified in fraction B2, with a value of 46.92%, expressed in DM. The in vitro digestibility coefficients (IVDC) of the DDGS nutrients did not differ (p > 0.05) in relation to those of the other experimental foods. The inclusion of DDGS in rations formulated for sheep did not change (p > 0.05) the IVDC of DM, OM, CP. NDF, or ADF, with mean values of 70.93, 70.64, 59.58, 52.83, and 43.40%, respectively. Therefore, DDGS comprise a protein-rich food containing more than 70% CP in fraction B2, with a large amount of carbohydrates bound to the cell wall. In addition, DDGS possess a similar digestibility coefficient to corn grain and soybean meal; however, up to 24% can be included in feed formulations for ruminants without changing the in vitro digestibility coefficient of nutrients.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26727
10.5433/1679-0359.2017v38n4p2029
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26727
identifier_str_mv 10.5433/1679-0359.2017v38n4p2029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26727/21379
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 2029-2040
Semina: Ciências Agrárias; v. 38 n. 4 (2017); 2029-2040
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
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instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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