Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11792 |
Resumo: | Many fungal enzymes have relevant applications in different industrial areas. The objective of this work was to select fungi producing hydrolytic enzymes, as well as establish agro-industrial wastes capable of inducing higher production levels. Xylanase, endoglucanase, amylase and poligalacturonase activities were determined by incubating the culture filtrates with their respective substrates. Subsequently, the reducing sugars determination was carried out using 3,5-dinitrosalicylic acid reagent.The protein determination was performed according the modified Bradford method. Among the fungal strains evaluated, Aspergillus niger J4 showed higher levels of xylanase production (8.73 ± 0.34 U/ml) and this was greatest when brewer’s spent grain was used as substrate (9.80 ± 0.02 U/ml). Penicillium miczynskii produced the highest levels of endoglucanasic activity (0.13 ± 0.03 U/ml), which, in turn, was favored in the pineapple peel presence (0.18 ± 0.02 U/ml). In relation to amylase, A. niger J26 was selected as the best producer strain (6.10 ± 0.30 U/ml) with wheat bran as the best substrate for their production (7.32 ± 0.14 U/ml). Penicillium verruculosum exhibited the highest level of poligalacturonase activity (8.65 ± 0.12 U/ml)), especially when grown in orange peel presence (10.32 ± 0.10 U/ml). These wastes use in these enzymes production may not only reduce their production cost, but also substantially reduce the environmental impact caused by the deposition of these wastes on the environment. |
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Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interestSeleção de fungos filamentosos e de resíduos agroindustriais para a produção de enzimas de interesse biotecnológicoFungal strainsHydrolasesLignocellulosic and amylaceous substratesPectic substances.Linhagens fúngicasHidrolasesSubstratos lignocelulósicosAmiláceosSubstâncias pécticas.Ciências AgráriasMany fungal enzymes have relevant applications in different industrial areas. The objective of this work was to select fungi producing hydrolytic enzymes, as well as establish agro-industrial wastes capable of inducing higher production levels. Xylanase, endoglucanase, amylase and poligalacturonase activities were determined by incubating the culture filtrates with their respective substrates. Subsequently, the reducing sugars determination was carried out using 3,5-dinitrosalicylic acid reagent.The protein determination was performed according the modified Bradford method. Among the fungal strains evaluated, Aspergillus niger J4 showed higher levels of xylanase production (8.73 ± 0.34 U/ml) and this was greatest when brewer’s spent grain was used as substrate (9.80 ± 0.02 U/ml). Penicillium miczynskii produced the highest levels of endoglucanasic activity (0.13 ± 0.03 U/ml), which, in turn, was favored in the pineapple peel presence (0.18 ± 0.02 U/ml). In relation to amylase, A. niger J26 was selected as the best producer strain (6.10 ± 0.30 U/ml) with wheat bran as the best substrate for their production (7.32 ± 0.14 U/ml). Penicillium verruculosum exhibited the highest level of poligalacturonase activity (8.65 ± 0.12 U/ml)), especially when grown in orange peel presence (10.32 ± 0.10 U/ml). These wastes use in these enzymes production may not only reduce their production cost, but also substantially reduce the environmental impact caused by the deposition of these wastes on the environment.Muitas enzimas produzidas por fungos têm relevantes aplicações em diferentes áreas industriais. O objetivo deste trabalho foi selecionar fungos filamentosos produtores de enzimas hidrolíticas, bem como estabelecer os resíduos agroindustriais capazes de induzir maiores níveis de produção. As atividades xilanásica, endoglucanásica, amilásica e poligalacturonásica foram determinadas incubandose os filtrados de cultura com seus respectivos substratos. Posteriormente, a determinação de açúcares redutores foi realizada utilizando-se o reagente ácido 3,5-dinitrosalicílico. A determinação de proteínas foi realizada segundo o método de Bradford modificado. Dentre as linhagens fúngicas avaliadas, Aspergillus niger J4 apresentou maiores níveis de produção de xilanases (8,73 ± 0,34 U/mL) e esta foi maior quando o bagaço de malte foi utilizado como substrato (9,80 ± 0,02 U/mL). Penicillium miczynskii produziu os índices mais elevados de atividade endoglucanásica (0,13 ± 0,03 U/mL), sendo está última favorecida na presença de casca de abacaxi (0,18 ± 0,02 U/mL). Em relação à amilase, A. niger J26 foi selecionada como a melhor linhagem produtora (6,10 ± 0,30 U/mL), sendo o farelo de trigo estabelecido como o melhor substrato indutor de sua produção (7,32 ± 0,14 U/mL). Penicillium verruculosum exibiu os maiores níveis de atividade poligalacturonásica (8,65 ± 0,12 U/mL), especialmente quando cultivado em presença de casca de laranja (10,32 ± 0,10 U/mL). O emprego destes resíduos no processo de produção destas enzimas poderá não apenas reduzir seus custos de produção, como também diminuir, substancialmente, o impacto ambiental causado pela deposição destes resíduos no ambiente.UEL2012-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1179210.5433/1679-0359.2012v33n6p2267Semina: Ciências Agrárias; Vol. 33 No. 6 (2012); 2267-2278Semina: Ciências Agrárias; v. 33 n. 6 (2012); 2267-22781679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11792/11683Copyright (c) 2012 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessStroparo, Erivelton CésarBeitel, Susan MichelzResende, Juliano Tadeu VilelaKnob, Adriana2023-01-27T13:07:39Zoai:ojs.pkp.sfu.ca:article/11792Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-27T13:07:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest Seleção de fungos filamentosos e de resíduos agroindustriais para a produção de enzimas de interesse biotecnológico |
title |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
spellingShingle |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest Stroparo, Erivelton César Fungal strains Hydrolases Lignocellulosic and amylaceous substrates Pectic substances. Linhagens fúngicas Hidrolases Substratos lignocelulósicos Amiláceos Substâncias pécticas. Ciências Agrárias |
title_short |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
title_full |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
title_fullStr |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
title_full_unstemmed |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
title_sort |
Filamentous fungi and agro-industrial residues selection for enzyme production of biotechnological interest |
author |
Stroparo, Erivelton César |
author_facet |
Stroparo, Erivelton César Beitel, Susan Michelz Resende, Juliano Tadeu Vilela Knob, Adriana |
author_role |
author |
author2 |
Beitel, Susan Michelz Resende, Juliano Tadeu Vilela Knob, Adriana |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Stroparo, Erivelton César Beitel, Susan Michelz Resende, Juliano Tadeu Vilela Knob, Adriana |
dc.subject.por.fl_str_mv |
Fungal strains Hydrolases Lignocellulosic and amylaceous substrates Pectic substances. Linhagens fúngicas Hidrolases Substratos lignocelulósicos Amiláceos Substâncias pécticas. Ciências Agrárias |
topic |
Fungal strains Hydrolases Lignocellulosic and amylaceous substrates Pectic substances. Linhagens fúngicas Hidrolases Substratos lignocelulósicos Amiláceos Substâncias pécticas. Ciências Agrárias |
description |
Many fungal enzymes have relevant applications in different industrial areas. The objective of this work was to select fungi producing hydrolytic enzymes, as well as establish agro-industrial wastes capable of inducing higher production levels. Xylanase, endoglucanase, amylase and poligalacturonase activities were determined by incubating the culture filtrates with their respective substrates. Subsequently, the reducing sugars determination was carried out using 3,5-dinitrosalicylic acid reagent.The protein determination was performed according the modified Bradford method. Among the fungal strains evaluated, Aspergillus niger J4 showed higher levels of xylanase production (8.73 ± 0.34 U/ml) and this was greatest when brewer’s spent grain was used as substrate (9.80 ± 0.02 U/ml). Penicillium miczynskii produced the highest levels of endoglucanasic activity (0.13 ± 0.03 U/ml), which, in turn, was favored in the pineapple peel presence (0.18 ± 0.02 U/ml). In relation to amylase, A. niger J26 was selected as the best producer strain (6.10 ± 0.30 U/ml) with wheat bran as the best substrate for their production (7.32 ± 0.14 U/ml). Penicillium verruculosum exhibited the highest level of poligalacturonase activity (8.65 ± 0.12 U/ml)), especially when grown in orange peel presence (10.32 ± 0.10 U/ml). These wastes use in these enzymes production may not only reduce their production cost, but also substantially reduce the environmental impact caused by the deposition of these wastes on the environment. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11792 10.5433/1679-0359.2012v33n6p2267 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11792 |
identifier_str_mv |
10.5433/1679-0359.2012v33n6p2267 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11792/11683 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2012 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2012 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 6 (2012); 2267-2278 Semina: Ciências Agrárias; v. 33 n. 6 (2012); 2267-2278 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306067081953280 |