Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29867 |
Resumo: | Porcine parvovirus 4 (PPV4) has been reported in several countries and the high rate of concurrent PCV2 infection with PPV4 may trigger the “porcine circovirus disease” (PCVD). This has awakened the interest in how PPV4 virus behaves. The aim of this study was to show that, like other swine viruses, the elimination of PPV4 in semen is intermittent. The study was conducted in the Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regional (Apta Regional). Over a period of six months, four boars were monitored and the semen was collected every 10 days, totalizing 74 semen samples. Through PCR testing, PPV4 was detected in 32/74 (43.2%) semen samples. Three sequences of 284 nt, showed identity varying from 98.2% to 100%, confirming the detection of the virus in all boars. Semen volume and sperm motility did not indicate significant difference between PPV4 positive or negative semen samples (p > 0.05). There was no significant difference between the monthly distributions of positive and negative samples. This is the first study showing intermittent shedding of PPV4 in naturally infected boars. Absence of typical clinical signs and/or influence in semen quantity was also observed. |
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Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first reportPrimeiro relato de eliminação intermitente de Parvovírus suíno 4 (PPV4) em sêmen de suíno infectado naturalmenteAnimal PPVPCRSeminal plasmaViral shedding.Excreção viralPCRPlasma seminalVírus animal.Porcine parvovirus 4 (PPV4) has been reported in several countries and the high rate of concurrent PCV2 infection with PPV4 may trigger the “porcine circovirus disease” (PCVD). This has awakened the interest in how PPV4 virus behaves. The aim of this study was to show that, like other swine viruses, the elimination of PPV4 in semen is intermittent. The study was conducted in the Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regional (Apta Regional). Over a period of six months, four boars were monitored and the semen was collected every 10 days, totalizing 74 semen samples. Through PCR testing, PPV4 was detected in 32/74 (43.2%) semen samples. Three sequences of 284 nt, showed identity varying from 98.2% to 100%, confirming the detection of the virus in all boars. Semen volume and sperm motility did not indicate significant difference between PPV4 positive or negative semen samples (p > 0.05). There was no significant difference between the monthly distributions of positive and negative samples. This is the first study showing intermittent shedding of PPV4 in naturally infected boars. Absence of typical clinical signs and/or influence in semen quantity was also observed.O Parvovírus suíno 4 (PPV4) foi descrito em vários países e a alta taxa de infecção simultânea entre PCV2 e PPV4 está associada ao desencadeamento da Circovirose. Isso despertou o interesse em estudar o comportamento do PPV4. O objetivo deste estudo foi mostrar que, assim como outros vírus suínos, a eliminação de PPV4 no sêmen é intermitente. O estudo foi realizado na Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regionais. Durante um período de seis meses, quatro cachaços foram monitorados e o sêmen foi coletado a cada 10 dias, totalizando 74 amostras de sêmen. Através de testes de PCR, o PPV4 foi detectado em 43,2% (32/74) das amostras de sêmen. Três sequências de 284 nt apresentaram 100% de identidade entre si, e quando, comparadas com sequências de PPV4 disponíveis no GenBank, mostraram uma identidade de 98.2% a 100%, confirmando a detecção do PPV4 nas amostras de sêmen dos cachaços. O volume de sêmen e a motilidade espermática não mostraram diferença significativa entre amostras positivas ou negativas de PPV4 (p > 0,05). Não houve diferença significativa entre as distribuições mensais de amostras positivas e negativas. Este é o primeiro estudo que mostra a eliminação intermitente de PPV4 em cachaços infectados naturalmente. Ausência de sinais clínicos típicos e / ou influência na quantidade de sêmen também foi observada.UEL2018-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2986710.5433/1679-0359.2018v39n5p2281Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 2281-2286Semina: Ciências Agrárias; v. 39 n. 5 (2018); 2281-22861679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29867/24308Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCastro, Alessandra Marnie Martins Gomes deBersano, Josete GarciaFeijó, Vanessa AparecidaOgata, Renato AkioNara, Julia MiticoOliveira, João Edson Faria deOliveira †, Roberto Carlos Vicente deBaldisseri Junior, Flavio Aparecido2022-10-20T18:27:32Zoai:ojs.pkp.sfu.ca:article/29867Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-20T18:27:32Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report Primeiro relato de eliminação intermitente de Parvovírus suíno 4 (PPV4) em sêmen de suíno infectado naturalmente |
title |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
spellingShingle |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report Castro, Alessandra Marnie Martins Gomes de Animal PPV PCR Seminal plasma Viral shedding. Excreção viral PCR Plasma seminal Vírus animal. |
title_short |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
title_full |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
title_fullStr |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
title_full_unstemmed |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
title_sort |
Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report |
author |
Castro, Alessandra Marnie Martins Gomes de |
author_facet |
Castro, Alessandra Marnie Martins Gomes de Bersano, Josete Garcia Feijó, Vanessa Aparecida Ogata, Renato Akio Nara, Julia Mitico Oliveira, João Edson Faria de Oliveira †, Roberto Carlos Vicente de Baldisseri Junior, Flavio Aparecido |
author_role |
author |
author2 |
Bersano, Josete Garcia Feijó, Vanessa Aparecida Ogata, Renato Akio Nara, Julia Mitico Oliveira, João Edson Faria de Oliveira †, Roberto Carlos Vicente de Baldisseri Junior, Flavio Aparecido |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Castro, Alessandra Marnie Martins Gomes de Bersano, Josete Garcia Feijó, Vanessa Aparecida Ogata, Renato Akio Nara, Julia Mitico Oliveira, João Edson Faria de Oliveira †, Roberto Carlos Vicente de Baldisseri Junior, Flavio Aparecido |
dc.subject.por.fl_str_mv |
Animal PPV PCR Seminal plasma Viral shedding. Excreção viral PCR Plasma seminal Vírus animal. |
topic |
Animal PPV PCR Seminal plasma Viral shedding. Excreção viral PCR Plasma seminal Vírus animal. |
description |
Porcine parvovirus 4 (PPV4) has been reported in several countries and the high rate of concurrent PCV2 infection with PPV4 may trigger the “porcine circovirus disease” (PCVD). This has awakened the interest in how PPV4 virus behaves. The aim of this study was to show that, like other swine viruses, the elimination of PPV4 in semen is intermittent. The study was conducted in the Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regional (Apta Regional). Over a period of six months, four boars were monitored and the semen was collected every 10 days, totalizing 74 semen samples. Through PCR testing, PPV4 was detected in 32/74 (43.2%) semen samples. Three sequences of 284 nt, showed identity varying from 98.2% to 100%, confirming the detection of the virus in all boars. Semen volume and sperm motility did not indicate significant difference between PPV4 positive or negative semen samples (p > 0.05). There was no significant difference between the monthly distributions of positive and negative samples. This is the first study showing intermittent shedding of PPV4 in naturally infected boars. Absence of typical clinical signs and/or influence in semen quantity was also observed. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29867 10.5433/1679-0359.2018v39n5p2281 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29867 |
identifier_str_mv |
10.5433/1679-0359.2018v39n5p2281 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29867/24308 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 39 No. 5 (2018); 2281-2286 Semina: Ciências Agrárias; v. 39 n. 5 (2018); 2281-2286 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306078226219008 |