No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetmic.2019.108512 http://hdl.handle.net/11449/198200 |
Resumo: | Bovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed. |
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No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boarsPestivirusSemenSeminal qualityViral sheddingBovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/nBiological Institute of São Paulo, Av. Conselheiro Rodrigues Alves, 1252 - Vila MarianaSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Biological Institute of São PauloStorino, Gabriel Yuri [UNESP]Xavier, Eduarda Bellini [UNESP]Mechler-Dreibi, Marina Lopes [UNESP]Simonatto, Artur [UNESP]Gatto, Igor Renan Honorato [UNESP]Oliveira, Maria Emilia Franco [UNESP]Pituco, Edviges Maristelade Oliveira, Luís Guilherme [UNESP]2020-12-12T01:06:20Z2020-12-12T01:06:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetmic.2019.108512Veterinary Microbiology, v. 240.1873-25420378-1135http://hdl.handle.net/11449/19820010.1016/j.vetmic.2019.1085122-s2.0-85075628394Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T09:55:30Zoai:repositorio.unesp.br:11449/198200Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:45:45.081326Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
title |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
spellingShingle |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars Storino, Gabriel Yuri [UNESP] Pestivirus Semen Seminal quality Viral shedding |
title_short |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
title_full |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
title_fullStr |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
title_full_unstemmed |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
title_sort |
No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars |
author |
Storino, Gabriel Yuri [UNESP] |
author_facet |
Storino, Gabriel Yuri [UNESP] Xavier, Eduarda Bellini [UNESP] Mechler-Dreibi, Marina Lopes [UNESP] Simonatto, Artur [UNESP] Gatto, Igor Renan Honorato [UNESP] Oliveira, Maria Emilia Franco [UNESP] Pituco, Edviges Maristela de Oliveira, Luís Guilherme [UNESP] |
author_role |
author |
author2 |
Xavier, Eduarda Bellini [UNESP] Mechler-Dreibi, Marina Lopes [UNESP] Simonatto, Artur [UNESP] Gatto, Igor Renan Honorato [UNESP] Oliveira, Maria Emilia Franco [UNESP] Pituco, Edviges Maristela de Oliveira, Luís Guilherme [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Biological Institute of São Paulo |
dc.contributor.author.fl_str_mv |
Storino, Gabriel Yuri [UNESP] Xavier, Eduarda Bellini [UNESP] Mechler-Dreibi, Marina Lopes [UNESP] Simonatto, Artur [UNESP] Gatto, Igor Renan Honorato [UNESP] Oliveira, Maria Emilia Franco [UNESP] Pituco, Edviges Maristela de Oliveira, Luís Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
Pestivirus Semen Seminal quality Viral shedding |
topic |
Pestivirus Semen Seminal quality Viral shedding |
description |
Bovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:06:20Z 2020-12-12T01:06:20Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetmic.2019.108512 Veterinary Microbiology, v. 240. 1873-2542 0378-1135 http://hdl.handle.net/11449/198200 10.1016/j.vetmic.2019.108512 2-s2.0-85075628394 |
url |
http://dx.doi.org/10.1016/j.vetmic.2019.108512 http://hdl.handle.net/11449/198200 |
identifier_str_mv |
Veterinary Microbiology, v. 240. 1873-2542 0378-1135 10.1016/j.vetmic.2019.108512 2-s2.0-85075628394 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128558414954496 |