No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars

Detalhes bibliográficos
Autor(a) principal: Storino, Gabriel Yuri [UNESP]
Data de Publicação: 2020
Outros Autores: Xavier, Eduarda Bellini [UNESP], Mechler-Dreibi, Marina Lopes [UNESP], Simonatto, Artur [UNESP], Gatto, Igor Renan Honorato [UNESP], Oliveira, Maria Emilia Franco [UNESP], Pituco, Edviges Maristela, de Oliveira, Luís Guilherme [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetmic.2019.108512
http://hdl.handle.net/11449/198200
Resumo: Bovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed.
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spelling No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boarsPestivirusSemenSeminal qualityViral sheddingBovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/nBiological Institute of São Paulo, Av. Conselheiro Rodrigues Alves, 1252 - Vila MarianaSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Biological Institute of São PauloStorino, Gabriel Yuri [UNESP]Xavier, Eduarda Bellini [UNESP]Mechler-Dreibi, Marina Lopes [UNESP]Simonatto, Artur [UNESP]Gatto, Igor Renan Honorato [UNESP]Oliveira, Maria Emilia Franco [UNESP]Pituco, Edviges Maristelade Oliveira, Luís Guilherme [UNESP]2020-12-12T01:06:20Z2020-12-12T01:06:20Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.vetmic.2019.108512Veterinary Microbiology, v. 240.1873-25420378-1135http://hdl.handle.net/11449/19820010.1016/j.vetmic.2019.1085122-s2.0-85075628394Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T09:55:30Zoai:repositorio.unesp.br:11449/198200Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:45:45.081326Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
title No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
spellingShingle No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
Storino, Gabriel Yuri [UNESP]
Pestivirus
Semen
Seminal quality
Viral shedding
title_short No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
title_full No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
title_fullStr No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
title_full_unstemmed No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
title_sort No effects of noncytopathic bovine viral diarrhea virus type 2 on the reproductive tract of experimentally inoculated boars
author Storino, Gabriel Yuri [UNESP]
author_facet Storino, Gabriel Yuri [UNESP]
Xavier, Eduarda Bellini [UNESP]
Mechler-Dreibi, Marina Lopes [UNESP]
Simonatto, Artur [UNESP]
Gatto, Igor Renan Honorato [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Pituco, Edviges Maristela
de Oliveira, Luís Guilherme [UNESP]
author_role author
author2 Xavier, Eduarda Bellini [UNESP]
Mechler-Dreibi, Marina Lopes [UNESP]
Simonatto, Artur [UNESP]
Gatto, Igor Renan Honorato [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Pituco, Edviges Maristela
de Oliveira, Luís Guilherme [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Biological Institute of São Paulo
dc.contributor.author.fl_str_mv Storino, Gabriel Yuri [UNESP]
Xavier, Eduarda Bellini [UNESP]
Mechler-Dreibi, Marina Lopes [UNESP]
Simonatto, Artur [UNESP]
Gatto, Igor Renan Honorato [UNESP]
Oliveira, Maria Emilia Franco [UNESP]
Pituco, Edviges Maristela
de Oliveira, Luís Guilherme [UNESP]
dc.subject.por.fl_str_mv Pestivirus
Semen
Seminal quality
Viral shedding
topic Pestivirus
Semen
Seminal quality
Viral shedding
description Bovine viral diarrhea virus (BVDV) infections in pigs may result in transient leukopenia, chronic gastroenteritis, septicemia, and hemorrhagic lesions. Both classical swine fever virus (CSF) and the atypical porcine pestivirus (APPV) are shed in the semen of infected boars. Because these viruses share conserved regions and present antigenic similarity, they may not be the only species belonging to the genus Pestivirus that can be shed in the semen of infected pigs. The objective of this study was to evaluate the testicular and epididymal changes, seminal parameters, and viral shedding in the reproductive tract of boars experimentally inoculated with noncytopathic BVDV-2. Six males were selected, and samples of blood, semen, and preputial swabs were collected every four days until the 52nd day after inoculation. The samples were tested for the presence of viral RNA by RT-PCR. An aliquot of whole blood was used to perform hematological analyses, which showed a significant reduction in monocyte counts and a significant increase in lymphocyte counts when comparing the pre- and postinoculation periods. The neutralizing antibody titers were determined by the virus neutralization test. None of the animals presented clinical signs or worsening of the seminal parameters that were evaluated. Moreover, BVDV-2 shedding by the reproductive route was not observed.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:06:20Z
2020-12-12T01:06:20Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetmic.2019.108512
Veterinary Microbiology, v. 240.
1873-2542
0378-1135
http://hdl.handle.net/11449/198200
10.1016/j.vetmic.2019.108512
2-s2.0-85075628394
url http://dx.doi.org/10.1016/j.vetmic.2019.108512
http://hdl.handle.net/11449/198200
identifier_str_mv Veterinary Microbiology, v. 240.
1873-2542
0378-1135
10.1016/j.vetmic.2019.108512
2-s2.0-85075628394
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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