Outbreak of Clostridium perfringens type D enterotoxemia in sheep
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32883 |
Resumo: | This work describes the first Brazilian laboratory-confirmed outbreak of enterotoxemia caused by Clostridium perfringens type D in sheep, which occurred in the state of Paraná. We address the epidemiological aspects involved, the diagnostic modalities employed, and the clinical signs and pathological findings observed. Eight healthy pregnant female sheep with no history of vaccination for clostridiosis presented with a history of abrupt feeding changes and neurological manifestations that quickly evolved to illness, coma and death. Four other females with clinical neurological signs were referred to the Veterinary Hospital of the Universidade Federal do Paraná, Palotina Sector. These animals presented with lethargy, motor incoordination, opisthotonus, pedal movements, muscle tremors, spastic paralysis, bruxism, mandibular trismus, sialorrhea, hyperexcitability and the inability to stand. They were examined and euthanized due to the seriousness of the clinical picture with an unfavorable prognosis. We performed gross anatomical and microscopic analyses of the organs and intestinal contents. We also performed bacterial isolation with molecular typing. From the intestinal contents, we detected toxins by means of the seroneutralization technique in mice. At necropsy, we noted pulmonary edema (2/4), necrotizing enteritis (4/4) and hyperemia of the leptomeninges (1/4). Microscopically, we observed lymphohistiocytic interstitial pneumonia, necrotic enteritis associated with the presence of rods, and nephrosis with interstitial lymphohistiocytic nephritis. No significant brain lesions were observed. Using serum neutralization, we identified epsilon toxin in the intestinal contents of all four animals. C. perfringens type D was identified. Based on the history, clinical signs, postmortem findings, and laboratory confirmation of the presence of epsilon toxin, we concluded that C. perfringens type D enterotoxemia caused this outbreak of sheep deaths. |
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Semina. Ciências Agrárias (Online) |
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Outbreak of Clostridium perfringens type D enterotoxemia in sheepSurto de enterotoxemia por Clostridium perfringens tipo D em ovinosClostridiosisEpsilon toxinNeurological signsOutbreak diagnosedSmall ruminant.ClostridiosesToxina épsilonSinais neurológicosSurto diagnosticadoPequenos ruminantes.This work describes the first Brazilian laboratory-confirmed outbreak of enterotoxemia caused by Clostridium perfringens type D in sheep, which occurred in the state of Paraná. We address the epidemiological aspects involved, the diagnostic modalities employed, and the clinical signs and pathological findings observed. Eight healthy pregnant female sheep with no history of vaccination for clostridiosis presented with a history of abrupt feeding changes and neurological manifestations that quickly evolved to illness, coma and death. Four other females with clinical neurological signs were referred to the Veterinary Hospital of the Universidade Federal do Paraná, Palotina Sector. These animals presented with lethargy, motor incoordination, opisthotonus, pedal movements, muscle tremors, spastic paralysis, bruxism, mandibular trismus, sialorrhea, hyperexcitability and the inability to stand. They were examined and euthanized due to the seriousness of the clinical picture with an unfavorable prognosis. We performed gross anatomical and microscopic analyses of the organs and intestinal contents. We also performed bacterial isolation with molecular typing. From the intestinal contents, we detected toxins by means of the seroneutralization technique in mice. At necropsy, we noted pulmonary edema (2/4), necrotizing enteritis (4/4) and hyperemia of the leptomeninges (1/4). Microscopically, we observed lymphohistiocytic interstitial pneumonia, necrotic enteritis associated with the presence of rods, and nephrosis with interstitial lymphohistiocytic nephritis. No significant brain lesions were observed. Using serum neutralization, we identified epsilon toxin in the intestinal contents of all four animals. C. perfringens type D was identified. Based on the history, clinical signs, postmortem findings, and laboratory confirmation of the presence of epsilon toxin, we concluded that C. perfringens type D enterotoxemia caused this outbreak of sheep deaths.Este trabalho descreve o primeiro surto brasileiro com confirmação laboratorial de enterotoxemia em ovinos por Clostridium perfringens tipo D, ocorrido no estado do Paraná, abordando os aspectos epidemiológicos envolvidos, sinais clínicos observados, achados patológicos e diagnóstico empregado. Oito fêmeas ovina, gestantes, em bom estado corporal e com histórico de mudanças bruscas na alimentação, sem histórico de vacinação para clostridioses, apresentaram manifestações clínicas neurológicas que logo evoluíram para decúbito, coma e morte. Outras quatro fêmeas apresentaram os sinais clínicos neurológicos e foram encaminhadas ao Hospital Veterinário da Universidade Federal do Paraná, setor Palotina. Os animais foram examinados e submetidos à eutanásia devido à gravidade do quadro clínico e prognóstico desfavorável. Destes quatro animais foram coletados fragmentos de órgãos e do conteúdo intestinal. Foi realizada análise macroscópica e microscópica dos órgãos, bem como isolamento bacteriano, tipificação molecular do agente e a detecção das toxinas por meio da técnica de soroneutralização em camundongos a partir do conteúdo intestinal. Os quatro ovinos examinados apresentaram letargia, incoordenação motora, opistótono, movimentos de pedalagem, tremores musculares, paralisia espástica, bruxismo, trismo mandibular, sialorréia, hiperexcitabilidade e decúbito. Na necropsia, constatou-se edema pulmonar (2/4), enterite necrohemorrágica (4/4) e hiperemia de leptomeninges (1/4). Microscopicamente havia pneumonia intersticial linfohistiocitária, enterite necrótica associada com a presença de bastonetes e nefrose com nefrite intersticial linfohistiocitária. Não foram observadas lesões encefálicas dignas de nota em função do quadro agudo da doença. Por meio da soroneutralização foi possível identificar a presença da toxina épsilon no conteúdo intestinal dos quatro animais em estudo. No isolamento e identificação microbiológica identificou-se C. perfringens tipo D. Diante do histórico, sinais clínicos, achados post-mpostmortem e a confirmação laboratorial da presença e identificação da toxina épsilon foi possível concluir o diagnóstico de enterotoxemia por C. perfringens tipo D como a causa do surto de mortes em ovinos no presente estudo.UEL2019-08-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRelato de casoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3288310.5433/1679-0359.2019v40n6p2593Semina: Ciências Agrárias; Vol. 40 No. 6 (2019); 2593-2602Semina: Ciências Agrárias; v. 40 n. 6 (2019); 2593-26021679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32883/25934Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSalvarani, Felipe MasieroFaccin, MayaneFreitas, Nayra Fernanda de Queiroz RamosMatos, Mônica Regina deGarcia, Edismair CarvalhoPagliosa, Geane MacielBarbosa, José DiomedesLobato, Francisco Carlos FariaViott, Aline de Marco2022-10-10T16:53:04Zoai:ojs.pkp.sfu.ca:article/32883Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:53:04Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep Surto de enterotoxemia por Clostridium perfringens tipo D em ovinos |
title |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
spellingShingle |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep Salvarani, Felipe Masiero Clostridiosis Epsilon toxin Neurological signs Outbreak diagnosed Small ruminant. Clostridioses Toxina épsilon Sinais neurológicos Surto diagnosticado Pequenos ruminantes. |
title_short |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
title_full |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
title_fullStr |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
title_full_unstemmed |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
title_sort |
Outbreak of Clostridium perfringens type D enterotoxemia in sheep |
author |
Salvarani, Felipe Masiero |
author_facet |
Salvarani, Felipe Masiero Faccin, Mayane Freitas, Nayra Fernanda de Queiroz Ramos Matos, Mônica Regina de Garcia, Edismair Carvalho Pagliosa, Geane Maciel Barbosa, José Diomedes Lobato, Francisco Carlos Faria Viott, Aline de Marco |
author_role |
author |
author2 |
Faccin, Mayane Freitas, Nayra Fernanda de Queiroz Ramos Matos, Mônica Regina de Garcia, Edismair Carvalho Pagliosa, Geane Maciel Barbosa, José Diomedes Lobato, Francisco Carlos Faria Viott, Aline de Marco |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Salvarani, Felipe Masiero Faccin, Mayane Freitas, Nayra Fernanda de Queiroz Ramos Matos, Mônica Regina de Garcia, Edismair Carvalho Pagliosa, Geane Maciel Barbosa, José Diomedes Lobato, Francisco Carlos Faria Viott, Aline de Marco |
dc.subject.por.fl_str_mv |
Clostridiosis Epsilon toxin Neurological signs Outbreak diagnosed Small ruminant. Clostridioses Toxina épsilon Sinais neurológicos Surto diagnosticado Pequenos ruminantes. |
topic |
Clostridiosis Epsilon toxin Neurological signs Outbreak diagnosed Small ruminant. Clostridioses Toxina épsilon Sinais neurológicos Surto diagnosticado Pequenos ruminantes. |
description |
This work describes the first Brazilian laboratory-confirmed outbreak of enterotoxemia caused by Clostridium perfringens type D in sheep, which occurred in the state of Paraná. We address the epidemiological aspects involved, the diagnostic modalities employed, and the clinical signs and pathological findings observed. Eight healthy pregnant female sheep with no history of vaccination for clostridiosis presented with a history of abrupt feeding changes and neurological manifestations that quickly evolved to illness, coma and death. Four other females with clinical neurological signs were referred to the Veterinary Hospital of the Universidade Federal do Paraná, Palotina Sector. These animals presented with lethargy, motor incoordination, opisthotonus, pedal movements, muscle tremors, spastic paralysis, bruxism, mandibular trismus, sialorrhea, hyperexcitability and the inability to stand. They were examined and euthanized due to the seriousness of the clinical picture with an unfavorable prognosis. We performed gross anatomical and microscopic analyses of the organs and intestinal contents. We also performed bacterial isolation with molecular typing. From the intestinal contents, we detected toxins by means of the seroneutralization technique in mice. At necropsy, we noted pulmonary edema (2/4), necrotizing enteritis (4/4) and hyperemia of the leptomeninges (1/4). Microscopically, we observed lymphohistiocytic interstitial pneumonia, necrotic enteritis associated with the presence of rods, and nephrosis with interstitial lymphohistiocytic nephritis. No significant brain lesions were observed. Using serum neutralization, we identified epsilon toxin in the intestinal contents of all four animals. C. perfringens type D was identified. Based on the history, clinical signs, postmortem findings, and laboratory confirmation of the presence of epsilon toxin, we concluded that C. perfringens type D enterotoxemia caused this outbreak of sheep deaths. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Relato de caso |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32883 10.5433/1679-0359.2019v40n6p2593 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32883 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6p2593 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32883/25934 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6 (2019); 2593-2602 Semina: Ciências Agrárias; v. 40 n. 6 (2019); 2593-2602 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306079686885376 |