In vitro multiplication of pear tree cultivar Cascatense
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20385 |
Resumo: | One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose. |
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In vitro multiplication of pear tree cultivar CascatenseMultiplicação in vitro de pereira cultivar CascatenseHimédia mediumFerrous sulfateFerric sulfateSucroseBenzylaminopurine.Meio HimédiaSulfato ferrosoSulfato férricoSacaroseBenzilaminopurina.One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose.Um dos principais entraves ao Desenvolvimento e Expansão da cultura da pereira no Brasil TEM SIDO Uma falta de cultivares adaptadas Às condições edafoclimáticas das Regiões potencialmente produtoras. Visando dificuldade ESTA Sanar, a Embrapa Clima Temperado desenvolveu e lançou Uma cv. Cascatense, com baixa exigencia em Frio (~ 400 h), Mais adequada Ao Clima do Rio Grande do Sul e de Santa Catarina. No entanto, mudas com Alta Qualidade Genética e Sanitária de São Necessárias Para garantir Uma boa Formação de hum pomar Produtivo. Plantas com Qualidade Sanitária e uniformidade PODEM Ser obtidas atraves da micropropagação in vitro. ASSIM, O Presente Trabalho Teve Como Objetivo VerificAR Uma Influência de Diferentes Meios de cultivo (MS, MS¾, WPM, QL e himedia), concentrações de sacarose (0, 15, 30, 45 e 60 g L-1) e benzilaminopurina - BAP (0; 0,4; 0,8; 1,2 e 1,6 mg L-1) na Multiplicação in vitro . da cv Cascatense, visando Obter hum protocolo Eficiente Para potencializar SUA PROPAGAÇÃO e reprodutibilidade. Os Estudos foram conduzidos em Três Experimentos sequenciais, nos Quais foram avaliados o Número de brotações POR explante, o comprimento das brotações, o Número de Gemas axilares POR brotação, massa fresca e massa seca das brotações. A cv. Cascatense PODE Ser propagada in vitro com a utilização do Meio himedia suplementado com 0,8 mg L-1 de BAP e 30 g L-1 de sacarose.UEL2016-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionScientific researchPesquisa Científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2038510.5433/1679-0359.2016v37n2p581Semina: Ciências Agrárias; Vol. 37 No. 2 (2016); 581-594Semina: Ciências Agrárias; v. 37 n. 2 (2016); 581-5941679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20385/18580http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Ilda Mariclei de Castro daCuchiara, Cristina CopsteinWinhelmann, Mara CíntiaBianchi, Valmor JoãoBraga, Eugenia Jacira BolacelDutra, Leonardo FerreiraPeters, José Antonio2022-12-01T16:02:02Zoai:ojs.pkp.sfu.ca:article/20385Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-01T16:02:02Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
In vitro multiplication of pear tree cultivar Cascatense Multiplicação in vitro de pereira cultivar Cascatense |
| title |
In vitro multiplication of pear tree cultivar Cascatense |
| spellingShingle |
In vitro multiplication of pear tree cultivar Cascatense Silva, Ilda Mariclei de Castro da Himédia medium Ferrous sulfate Ferric sulfate Sucrose Benzylaminopurine. Meio Himédia Sulfato ferroso Sulfato férrico Sacarose Benzilaminopurina. |
| title_short |
In vitro multiplication of pear tree cultivar Cascatense |
| title_full |
In vitro multiplication of pear tree cultivar Cascatense |
| title_fullStr |
In vitro multiplication of pear tree cultivar Cascatense |
| title_full_unstemmed |
In vitro multiplication of pear tree cultivar Cascatense |
| title_sort |
In vitro multiplication of pear tree cultivar Cascatense |
| author |
Silva, Ilda Mariclei de Castro da |
| author_facet |
Silva, Ilda Mariclei de Castro da Cuchiara, Cristina Copstein Winhelmann, Mara Cíntia Bianchi, Valmor João Braga, Eugenia Jacira Bolacel Dutra, Leonardo Ferreira Peters, José Antonio |
| author_role |
author |
| author2 |
Cuchiara, Cristina Copstein Winhelmann, Mara Cíntia Bianchi, Valmor João Braga, Eugenia Jacira Bolacel Dutra, Leonardo Ferreira Peters, José Antonio |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Ilda Mariclei de Castro da Cuchiara, Cristina Copstein Winhelmann, Mara Cíntia Bianchi, Valmor João Braga, Eugenia Jacira Bolacel Dutra, Leonardo Ferreira Peters, José Antonio |
| dc.subject.por.fl_str_mv |
Himédia medium Ferrous sulfate Ferric sulfate Sucrose Benzylaminopurine. Meio Himédia Sulfato ferroso Sulfato férrico Sacarose Benzilaminopurina. |
| topic |
Himédia medium Ferrous sulfate Ferric sulfate Sucrose Benzylaminopurine. Meio Himédia Sulfato ferroso Sulfato férrico Sacarose Benzilaminopurina. |
| description |
One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-04-26 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Scientific research Pesquisa Científica |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20385 10.5433/1679-0359.2016v37n2p581 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20385 |
| identifier_str_mv |
10.5433/1679-0359.2016v37n2p581 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20385/18580 |
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http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
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UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 37 No. 2 (2016); 581-594 Semina: Ciências Agrárias; v. 37 n. 2 (2016); 581-594 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Universidade Estadual de Londrina (UEL) |
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UEL |
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UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1799306073322029056 |