Hemoglobin polymorphism in Hampshire Down sheep herd

Detalhes bibliográficos
Autor(a) principal: Rego, Fabíola Cristine de Almeida
Data de Publicação: 2011
Outros Autores: Junior, Flavio Antonio Barca, Cunha Filho, Luiz Fernando Coelho, Okano, Werner, Silva, Luis César da, Ludovico, Agostinho, Trapp, Silvia Manduca, Santana, Elsa, Tanaka, Neide Mariko
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3028
Resumo: The present study aimed to determine the types of hemoglobin in Hampshire Down crossbreed sheep, and verify that this locus is in Hardy-Weinberg equilibrium. There have been collected 46 blood samples of healthy Hampshire Down crossbreed sheep. These samples were used to separate the hemoglobin per electrophoresis. The electrophoresis of the hemoglobin revealed a slow band characterized as hemoglobin A (HbAA), a fast band characterized as hemoglobin B (HbBB) and two bands in the heterozygous hemoglobin A and B (HbAB). The HbAB type was the most frequently one, followed by hemoglobin A (HbAA) and B (HbBB). The genotypic frequency of individuals BB, AB and AA were 36,95; 54,35 and 8,70% respectively. The allelic frequency of A and B were respectively 35,87% and 64,13%.. The qui square test (?2 = 0.859 and p = 0.6509) confirmed that the tested locus is in Hardy-Weinberg equilibrium.
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spelling Hemoglobin polymorphism in Hampshire Down sheep herdPolimorfismo de hemoglobina em rebanho de ovinos Hampshire DownEElectrophoresisHemoglobin polymorphismSheep.EletroforesePolimorfismo de hemoglobinaOvinos.The present study aimed to determine the types of hemoglobin in Hampshire Down crossbreed sheep, and verify that this locus is in Hardy-Weinberg equilibrium. There have been collected 46 blood samples of healthy Hampshire Down crossbreed sheep. These samples were used to separate the hemoglobin per electrophoresis. The electrophoresis of the hemoglobin revealed a slow band characterized as hemoglobin A (HbAA), a fast band characterized as hemoglobin B (HbBB) and two bands in the heterozygous hemoglobin A and B (HbAB). The HbAB type was the most frequently one, followed by hemoglobin A (HbAA) and B (HbBB). The genotypic frequency of individuals BB, AB and AA were 36,95; 54,35 and 8,70% respectively. The allelic frequency of A and B were respectively 35,87% and 64,13%.. The qui square test (?2 = 0.859 and p = 0.6509) confirmed that the tested locus is in Hardy-Weinberg equilibrium.O presente trabalho teve como objetivo determinar os tipos de hemoglobinas em ovinos Hampshire Down e verificar se este locus encontra-se em equilíbrio de Hardy-Weinberg. Foram coletadas 46 amostras de sangue de ovinos Hampshire Down, considerados clinicamente sadios. Estas amostras foram utilizadas para a separação das hemoglobinas por eletroforese. A eletroforese das hemoglobinas revelou uma banda lenta caracterizada como hemoglobina B (HbBB), uma banda rápida caracterizada como hemoglobina A (HbAA) e duas bandas no heterozigoto para hemoglobinas A e B (HbAB). A variante HbAB foi a mais freqüentemente encontrada, seguida pela hemoglobina A (HbAA) e B (HbBB). A freqüência genotípica dos indivíduos BB, AB e AA foram 36,95; 54,35 e 8,70% respectivamente. A freqüência alélica de A e B foram respectivamente 35,87% e 64,13%. Pelo teste do qui-quadrado realizado (?2 = 0,859 e p=0,6509) confirmou-se que o locus testado está em equilíbrio de Hardy-Weinberg.UEL2011-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/302810.5433/1679-0359.2011v32n1p261Semina: Ciências Agrárias; Vol. 32 No. 1 (2011); 261-266Semina: Ciências Agrárias; v. 32 n. 1 (2011); 261-2661679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3028/7192Rego, Fabíola Cristine de AlmeidaJunior, Flavio Antonio BarcaCunha Filho, Luiz Fernando CoelhoOkano, WernerSilva, Luis César daLudovico, AgostinhoTrapp, Silvia ManducaSantana, ElsaTanaka, Neide Marikoinfo:eu-repo/semantics/openAccess2015-11-19T18:38:02Zoai:ojs.pkp.sfu.ca:article/3028Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:02Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Hemoglobin polymorphism in Hampshire Down sheep herd
Polimorfismo de hemoglobina em rebanho de ovinos Hampshire Down
title Hemoglobin polymorphism in Hampshire Down sheep herd
spellingShingle Hemoglobin polymorphism in Hampshire Down sheep herd
Rego, Fabíola Cristine de Almeida
EElectrophoresis
Hemoglobin polymorphism
Sheep.
Eletroforese
Polimorfismo de hemoglobina
Ovinos.
title_short Hemoglobin polymorphism in Hampshire Down sheep herd
title_full Hemoglobin polymorphism in Hampshire Down sheep herd
title_fullStr Hemoglobin polymorphism in Hampshire Down sheep herd
title_full_unstemmed Hemoglobin polymorphism in Hampshire Down sheep herd
title_sort Hemoglobin polymorphism in Hampshire Down sheep herd
author Rego, Fabíola Cristine de Almeida
author_facet Rego, Fabíola Cristine de Almeida
Junior, Flavio Antonio Barca
Cunha Filho, Luiz Fernando Coelho
Okano, Werner
Silva, Luis César da
Ludovico, Agostinho
Trapp, Silvia Manduca
Santana, Elsa
Tanaka, Neide Mariko
author_role author
author2 Junior, Flavio Antonio Barca
Cunha Filho, Luiz Fernando Coelho
Okano, Werner
Silva, Luis César da
Ludovico, Agostinho
Trapp, Silvia Manduca
Santana, Elsa
Tanaka, Neide Mariko
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rego, Fabíola Cristine de Almeida
Junior, Flavio Antonio Barca
Cunha Filho, Luiz Fernando Coelho
Okano, Werner
Silva, Luis César da
Ludovico, Agostinho
Trapp, Silvia Manduca
Santana, Elsa
Tanaka, Neide Mariko
dc.subject.por.fl_str_mv EElectrophoresis
Hemoglobin polymorphism
Sheep.
Eletroforese
Polimorfismo de hemoglobina
Ovinos.
topic EElectrophoresis
Hemoglobin polymorphism
Sheep.
Eletroforese
Polimorfismo de hemoglobina
Ovinos.
description The present study aimed to determine the types of hemoglobin in Hampshire Down crossbreed sheep, and verify that this locus is in Hardy-Weinberg equilibrium. There have been collected 46 blood samples of healthy Hampshire Down crossbreed sheep. These samples were used to separate the hemoglobin per electrophoresis. The electrophoresis of the hemoglobin revealed a slow band characterized as hemoglobin A (HbAA), a fast band characterized as hemoglobin B (HbBB) and two bands in the heterozygous hemoglobin A and B (HbAB). The HbAB type was the most frequently one, followed by hemoglobin A (HbAA) and B (HbBB). The genotypic frequency of individuals BB, AB and AA were 36,95; 54,35 and 8,70% respectively. The allelic frequency of A and B were respectively 35,87% and 64,13%.. The qui square test (?2 = 0.859 and p = 0.6509) confirmed that the tested locus is in Hardy-Weinberg equilibrium.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3028
10.5433/1679-0359.2011v32n1p261
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3028
identifier_str_mv 10.5433/1679-0359.2011v32n1p261
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3028/7192
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 32 No. 1 (2011); 261-266
Semina: Ciências Agrárias; v. 32 n. 1 (2011); 261-266
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306059675860992

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