Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496 |
Resumo: | Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria. |
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Semina. Ciências Agrárias (Online) |
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Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistanceAvaliação do óleo essencial de alecrim (Rosmarinus officinalis L.) como modulador da resistência bacterianaEssential oilRosemaryBacterial resistance.5.07.01.00-2Óleo essencialAlecrimResistência bacteriana.5.07.01.00-2Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria.Diversos microrganismos podem ser veiculados por alimentos causando doenças nos seres humanos. Os antibióticos comumente utilizados no tratamento dessas doenças têm apresentado baixo ou nenhum efeito, tendo em vista à resistência que muitos microrganismos têm adquirido. Diante deste quadro, o objetivo desta pesquisa foi avaliar o óleo essencial das folhas de Rosmarinus officinalis L. como modulador da resistência bacteriana a drogas. O óleo essencial foi obtido através de hidrodestilação em aparelho de Clevenger por 3h. Foram testadas 4 cepas de E.coli resistentes a Amplicilina (AMP) e a Tetraciclina (TET) e 4 cepas de Salmonella spp. resistentes a Nitrofurantoína (NIT). As cepas em suspensão escala MacFarland 0,5 foram inoculadas em agar Mueller Hinton, em seguida os discos dos antibióticos embebidos com 10 e 20?L do óleo de alecrim puro foram dispostos sobre as placas. Após 24h/37ºC foram medidos os halos ao redor dos discos. Todas as cepas avaliadas apresentaram susceptibilidade à ação combinada do óleo essencial com os antibióticos testados. Os resultados obtidos indicam ser promissora a utilização de óleo essencial de alecrim em associação com antibióticos no combate a bactérias patogênicas.UEL2012-05-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/749610.5433/1679-0359.2012v33n2p687Semina: Ciências Agrárias; Vol. 33 No. 2 (2012); 687-696Semina: Ciências Agrárias; v. 33 n. 2 (2012); 687-6961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496/10452Ribeiro, Daniele SilvaMelo, Daniela BenevidesGuimarães, Alaíse GilVelozo, Eudes Silvainfo:eu-repo/semantics/openAccess2015-11-19T18:37:37Zoai:ojs.pkp.sfu.ca:article/7496Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance Avaliação do óleo essencial de alecrim (Rosmarinus officinalis L.) como modulador da resistência bacteriana |
title |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
spellingShingle |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance Ribeiro, Daniele Silva Essential oil Rosemary Bacterial resistance. 5.07.01.00-2 Óleo essencial Alecrim Resistência bacteriana. 5.07.01.00-2 |
title_short |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
title_full |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
title_fullStr |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
title_full_unstemmed |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
title_sort |
Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance |
author |
Ribeiro, Daniele Silva |
author_facet |
Ribeiro, Daniele Silva Melo, Daniela Benevides Guimarães, Alaíse Gil Velozo, Eudes Silva |
author_role |
author |
author2 |
Melo, Daniela Benevides Guimarães, Alaíse Gil Velozo, Eudes Silva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Daniele Silva Melo, Daniela Benevides Guimarães, Alaíse Gil Velozo, Eudes Silva |
dc.subject.por.fl_str_mv |
Essential oil Rosemary Bacterial resistance. 5.07.01.00-2 Óleo essencial Alecrim Resistência bacteriana. 5.07.01.00-2 |
topic |
Essential oil Rosemary Bacterial resistance. 5.07.01.00-2 Óleo essencial Alecrim Resistência bacteriana. 5.07.01.00-2 |
description |
Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496 10.5433/1679-0359.2012v33n2p687 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496 |
identifier_str_mv |
10.5433/1679-0359.2012v33n2p687 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496/10452 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 33 No. 2 (2012); 687-696 Semina: Ciências Agrárias; v. 33 n. 2 (2012); 687-696 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306064326295552 |