Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)

• Autor(a) principal: Lopera-Barrero, Nelson Mauricio
• Data de Publicação: 2019
• Outros Autores: Souza, Felipe Pinheiro de, Lima, Ed Christian Suzuki de, Urrea-Rojas, Angela Maria, Castro, Pedro Luiz de, Goes, Elenice Souza dos Reis, Pandolfi, Victor César Freitas, Yamachita, Andrei Lincoln, Oliveira, Carlos Antonio Lopes de, Leite, Natália Gonçalves, Ribeiro, Ricardo Pereira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Semina. Ciências Agrárias (Online)
• Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727
• topic: Brycon orbignyanus; Conservation; Genetic variability; Microsatellites; Piaractus mesopotamicus; Prochilodus lineatus.; Brycon orbignyanus; Conservação; Microssatélites; Piaractus mesopotamicus; Prochilodus lineatus; Variabilidade genética.

In recent years, anthropogenic factors such as pollution, overfishing, and construction of hydroelectric plants have significantly impacted natural fish populations. Research focusing on genetically evaluation of these impacts is necessary to objectively target conservation programs. The aim of this study was to evaluate the genetic diversity of Curimba (Prochilodus lineatus), Pacu (Piaractus mesopotamicus), and Piracanjuba (Brycon orbignyanus) populations from the Água Vermelha Reservoir, Rio Grande-SP. Microsatellite loci were amplified, producing 56, 24, and 26 alleles for the populations of the three species, respectively. The number of alleles per locus ranged from three to ten for P. lineatus, two to five for P. mesopotamicus, and two to four for B. orbignyanus. The observed heterozygosity (Ho) was higher in the P. lineatus population (0.547), relative to the P. mesopotamicus and B. orbignyanus populations (0.473 and 0.527, respectively). The mean values of Ho were lower than the average expected heterozygosity (He) in the three species, being corroborated by the positive inbreeding coefficient (Fis). Deviations from the Hardy-Weinberg equilibrium (HWE) were found in five, three, and two loci for P. lineatus, P. mesopotamicus, and B. orbignyanus, respectively. Wilcoxon tests revealed recent bottlenecks in the three species, evidenced by a significant excess of heterozygotes (p < 0.05) detected only in the Infinite Allele Model (IAM). In conclusion, adequate genetic variability was observed in the three populations with the presence of heterozygous deficits.