Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)

Detalhes bibliográficos
Autor(a) principal: Lopera-Barrero, Nelson Mauricio
Data de Publicação: 2019
Outros Autores: Souza, Felipe Pinheiro de, Lima, Ed Christian Suzuki de, Urrea-Rojas, Angela Maria, Castro, Pedro Luiz de, Goes, Elenice Souza dos Reis, Pandolfi, Victor César Freitas, Yamachita, Andrei Lincoln, Oliveira, Carlos Antonio Lopes de, Leite, Natália Gonçalves, Ribeiro, Ricardo Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727
Resumo: In recent years, anthropogenic factors such as pollution, overfishing, and construction of hydroelectric plants have significantly impacted natural fish populations. Research focusing on genetically evaluation of these impacts is necessary to objectively target conservation programs. The aim of this study was to evaluate the genetic diversity of Curimba (Prochilodus lineatus), Pacu (Piaractus mesopotamicus), and Piracanjuba (Brycon orbignyanus) populations from the Água Vermelha Reservoir, Rio Grande-SP. Microsatellite loci were amplified, producing 56, 24, and 26 alleles for the populations of the three species, respectively. The number of alleles per locus ranged from three to ten for P. lineatus, two to five for P. mesopotamicus, and two to four for B. orbignyanus. The observed heterozygosity (Ho) was higher in the P. lineatus population (0.547), relative to the P. mesopotamicus and B. orbignyanus populations (0.473 and 0.527, respectively). The mean values of Ho were lower than the average expected heterozygosity (He) in the three species, being corroborated by the positive inbreeding coefficient (Fis). Deviations from the Hardy-Weinberg equilibrium (HWE) were found in five, three, and two loci for P. lineatus, P. mesopotamicus, and B. orbignyanus, respectively. Wilcoxon tests revealed recent bottlenecks in the three species, evidenced by a significant excess of heterozygotes (p < 0.05) detected only in the Infinite Allele Model (IAM). In conclusion, adequate genetic variability was observed in the three populations with the presence of heterozygous deficits.
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spelling Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)Diversidade genética de populações de peixes migratórios do reservatório do Rio Grande (São Paulo, Brasil)Brycon orbignyanusConservationGenetic variabilityMicrosatellitesPiaractus mesopotamicusProchilodus lineatus.Brycon orbignyanusConservaçãoMicrossatélitesPiaractus mesopotamicusProchilodus lineatusVariabilidade genética.In recent years, anthropogenic factors such as pollution, overfishing, and construction of hydroelectric plants have significantly impacted natural fish populations. Research focusing on genetically evaluation of these impacts is necessary to objectively target conservation programs. The aim of this study was to evaluate the genetic diversity of Curimba (Prochilodus lineatus), Pacu (Piaractus mesopotamicus), and Piracanjuba (Brycon orbignyanus) populations from the Água Vermelha Reservoir, Rio Grande-SP. Microsatellite loci were amplified, producing 56, 24, and 26 alleles for the populations of the three species, respectively. The number of alleles per locus ranged from three to ten for P. lineatus, two to five for P. mesopotamicus, and two to four for B. orbignyanus. The observed heterozygosity (Ho) was higher in the P. lineatus population (0.547), relative to the P. mesopotamicus and B. orbignyanus populations (0.473 and 0.527, respectively). The mean values of Ho were lower than the average expected heterozygosity (He) in the three species, being corroborated by the positive inbreeding coefficient (Fis). Deviations from the Hardy-Weinberg equilibrium (HWE) were found in five, three, and two loci for P. lineatus, P. mesopotamicus, and B. orbignyanus, respectively. Wilcoxon tests revealed recent bottlenecks in the three species, evidenced by a significant excess of heterozygotes (p < 0.05) detected only in the Infinite Allele Model (IAM). In conclusion, adequate genetic variability was observed in the three populations with the presence of heterozygous deficits.Nos últimos anos, fatores antrópicos como poluição, sobrepesca e construção de hidrelétricas têm impactado significativamente as populações naturais de peixes. Pesquisas que permitam avaliar geneticamente esse impacto são necessárias para orientar objetivamente programas de conservação. O objetivo do presente estudo foi avaliar a diversidade genética de populações de Curimba (Prochilodus lineatus), Pacu (Piaractus mesopotamicus) e Piracanjuba (Brycon orbignyanus) provenientes do reservatório de Água Vermelha, Rio Grande-SP. Foram amplificados loci microssatélites que produziram 56, 24 e 26 alelos para as populações das três espécies, respectivamente. O número de alelos por locus variou de três a dez para P. lineatus, dois a cinco para P. mesopotamicus e dois a quatro para B. orbignianus. A heterozigosidade observada (Ho) foi mais elevada na população de P. lineatus (0,547) em relação às populações de P. mesopotamicus e B. orbignyanus (0,473 e 0,527, respectivamente). A média dos valores de Ho foram inferiores à média de He nas três espécies sendo corroborado pelo Fis positivo observado. Foi encontrado desvio do equilíbrio de Hardy-Weinberg (HWE) em cinco loci para P. lineatus, três loci para P. mesopotamicus e dois em B. orbignyanus. A análise de bottleneck revelou excesso de heterozigotos (p < 0,05) pelo teste de Wilcoxon nas três espécies apenas no modelo Infinite Allele Model (IAM). Foi observada adequada variabilidade genética nas três populações com a presença de déficit de heterozigotos.UEL2019-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3272710.5433/1679-0359.2019v40n1p503Semina: Ciências Agrárias; Vol. 40 No. 1 (2019); 503-510Semina: Ciências Agrárias; v. 40 n. 1 (2019); 503-5101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727/25025Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLopera-Barrero, Nelson MauricioSouza, Felipe Pinheiro deLima, Ed Christian Suzuki deUrrea-Rojas, Angela MariaCastro, Pedro Luiz deGoes, Elenice Souza dos ReisPandolfi, Victor César FreitasYamachita, Andrei LincolnOliveira, Carlos Antonio Lopes deLeite, Natália GonçalvesRibeiro, Ricardo Pereira2022-10-19T15:38:26Zoai:ojs.pkp.sfu.ca:article/32727Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T15:38:26Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
Diversidade genética de populações de peixes migratórios do reservatório do Rio Grande (São Paulo, Brasil)
title Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
spellingShingle Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
Lopera-Barrero, Nelson Mauricio
Brycon orbignyanus
Conservation
Genetic variability
Microsatellites
Piaractus mesopotamicus
Prochilodus lineatus.
Brycon orbignyanus
Conservação
Microssatélites
Piaractus mesopotamicus
Prochilodus lineatus
Variabilidade genética.
title_short Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
title_full Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
title_fullStr Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
title_full_unstemmed Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
title_sort Genetic diversity of migratory fish populations of the Rio Grande Reservoir (São Paulo, Brazil)
author Lopera-Barrero, Nelson Mauricio
author_facet Lopera-Barrero, Nelson Mauricio
Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Urrea-Rojas, Angela Maria
Castro, Pedro Luiz de
Goes, Elenice Souza dos Reis
Pandolfi, Victor César Freitas
Yamachita, Andrei Lincoln
Oliveira, Carlos Antonio Lopes de
Leite, Natália Gonçalves
Ribeiro, Ricardo Pereira
author_role author
author2 Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Urrea-Rojas, Angela Maria
Castro, Pedro Luiz de
Goes, Elenice Souza dos Reis
Pandolfi, Victor César Freitas
Yamachita, Andrei Lincoln
Oliveira, Carlos Antonio Lopes de
Leite, Natália Gonçalves
Ribeiro, Ricardo Pereira
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopera-Barrero, Nelson Mauricio
Souza, Felipe Pinheiro de
Lima, Ed Christian Suzuki de
Urrea-Rojas, Angela Maria
Castro, Pedro Luiz de
Goes, Elenice Souza dos Reis
Pandolfi, Victor César Freitas
Yamachita, Andrei Lincoln
Oliveira, Carlos Antonio Lopes de
Leite, Natália Gonçalves
Ribeiro, Ricardo Pereira
dc.subject.por.fl_str_mv Brycon orbignyanus
Conservation
Genetic variability
Microsatellites
Piaractus mesopotamicus
Prochilodus lineatus.
Brycon orbignyanus
Conservação
Microssatélites
Piaractus mesopotamicus
Prochilodus lineatus
Variabilidade genética.
topic Brycon orbignyanus
Conservation
Genetic variability
Microsatellites
Piaractus mesopotamicus
Prochilodus lineatus.
Brycon orbignyanus
Conservação
Microssatélites
Piaractus mesopotamicus
Prochilodus lineatus
Variabilidade genética.
description In recent years, anthropogenic factors such as pollution, overfishing, and construction of hydroelectric plants have significantly impacted natural fish populations. Research focusing on genetically evaluation of these impacts is necessary to objectively target conservation programs. The aim of this study was to evaluate the genetic diversity of Curimba (Prochilodus lineatus), Pacu (Piaractus mesopotamicus), and Piracanjuba (Brycon orbignyanus) populations from the Água Vermelha Reservoir, Rio Grande-SP. Microsatellite loci were amplified, producing 56, 24, and 26 alleles for the populations of the three species, respectively. The number of alleles per locus ranged from three to ten for P. lineatus, two to five for P. mesopotamicus, and two to four for B. orbignyanus. The observed heterozygosity (Ho) was higher in the P. lineatus population (0.547), relative to the P. mesopotamicus and B. orbignyanus populations (0.473 and 0.527, respectively). The mean values of Ho were lower than the average expected heterozygosity (He) in the three species, being corroborated by the positive inbreeding coefficient (Fis). Deviations from the Hardy-Weinberg equilibrium (HWE) were found in five, three, and two loci for P. lineatus, P. mesopotamicus, and B. orbignyanus, respectively. Wilcoxon tests revealed recent bottlenecks in the three species, evidenced by a significant excess of heterozygotes (p < 0.05) detected only in the Infinite Allele Model (IAM). In conclusion, adequate genetic variability was observed in the three populations with the presence of heterozygous deficits.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727
10.5433/1679-0359.2019v40n1p503
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727
identifier_str_mv 10.5433/1679-0359.2019v40n1p503
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/32727/25025
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 1 (2019); 503-510
Semina: Ciências Agrárias; v. 40 n. 1 (2019); 503-510
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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