Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean

Detalhes bibliográficos
Autor(a) principal: Medeiros, Camila de
Data de Publicação: 2020
Outros Autores: Pereira, Gilberto Aguiar, Freitas, Janyeli Dorini Silva de, Oliveira Filho, Olavo Bilac Quaresma de, Valle, Juliana Silveira do, Linde, Giani Andrea, Paccola-Meirelles, Luzia Doretto, Colauto, Nelson Barros, Barcellos, Fernando Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281
Resumo: Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus.
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spelling Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybeanCaracterização dos genes de cepas de Bradyrhizobium spp. contrastantes quanto à eficiência de fixação biológica de nitrogênio em sojaBradyrhizobiumRhizobacteriaPCR-RFLPRestriction Polymorphisms.BradyrhizobiumRizobactériasPCR-RFLPPolimorfismo de restrição.Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus.Bactérias do gênero Bradyrhizobium podem estabelecer simbiose com a soja e suprir a demanda de nitrogênio pela fixação biológica de nitrogênio (FBN). Este estudo teve como objetivo caracterizar genes relacionados à eficiência na FBN em cepas de B. japonicum contrastantes quanto a eficiência na FBN. As sequências gênicas relacionadas à eficiência da FBN em soja foram previamente identificadas na cepa S370 de B. japonicum utilizando uma técnica de DNA subtrativo. Os genes foram amplificados com iniciadores construídos a partir do genoma da cepa USDA 110. Os produtos de PCR foram digeridos com endonucleases de restrição e os produtos de RFLP foram analisados por eletroforese horizontal. Dos quatro genes estudados, apenas blr 3208 e blr 4511 amplificaram na maioria das cepas. Não foi observado polimorfismo no perfil de restrição dos genes blr 3208 e blr 4511 por PCR-RFLP. As cepas contrastantes tiveram os genes blr 3208 e blr 4511 sequenciados e a comparação das sequências nucleotídicas por análise de alinhamento múltiplo mostrou a presença de regiões internas preservadas, confirmando a análise realizada por PCR-RFLP. Os genes blr 3208 e blr 4511 são altamente conservados entre as cepas de B. japonicum, o que pode estar relacionado à função adaptativa durante o processo evolutivo do gênero Bradyrhizobium.UEL2020-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4028110.5433/1679-0359.2020v41n6Supl2p3067Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3067-3080Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3067-30801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281/28262Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMedeiros, Camila dePereira, Gilberto AguiarFreitas, Janyeli Dorini Silva deOliveira Filho, Olavo Bilac Quaresma deValle, Juliana Silveira doLinde, Giani AndreaPaccola-Meirelles, Luzia DorettoColauto, Nelson BarrosBarcellos, Fernando Gomes2022-10-04T16:31:27Zoai:ojs.pkp.sfu.ca:article/40281Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T16:31:27Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
Caracterização dos genes de cepas de Bradyrhizobium spp. contrastantes quanto à eficiência de fixação biológica de nitrogênio em soja
title Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
spellingShingle Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
Medeiros, Camila de
Bradyrhizobium
Rhizobacteria
PCR-RFLP
Restriction Polymorphisms.
Bradyrhizobium
Rizobactérias
PCR-RFLP
Polimorfismo de restrição.
title_short Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
title_full Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
title_fullStr Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
title_full_unstemmed Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
title_sort Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
author Medeiros, Camila de
author_facet Medeiros, Camila de
Pereira, Gilberto Aguiar
Freitas, Janyeli Dorini Silva de
Oliveira Filho, Olavo Bilac Quaresma de
Valle, Juliana Silveira do
Linde, Giani Andrea
Paccola-Meirelles, Luzia Doretto
Colauto, Nelson Barros
Barcellos, Fernando Gomes
author_role author
author2 Pereira, Gilberto Aguiar
Freitas, Janyeli Dorini Silva de
Oliveira Filho, Olavo Bilac Quaresma de
Valle, Juliana Silveira do
Linde, Giani Andrea
Paccola-Meirelles, Luzia Doretto
Colauto, Nelson Barros
Barcellos, Fernando Gomes
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Medeiros, Camila de
Pereira, Gilberto Aguiar
Freitas, Janyeli Dorini Silva de
Oliveira Filho, Olavo Bilac Quaresma de
Valle, Juliana Silveira do
Linde, Giani Andrea
Paccola-Meirelles, Luzia Doretto
Colauto, Nelson Barros
Barcellos, Fernando Gomes
dc.subject.por.fl_str_mv Bradyrhizobium
Rhizobacteria
PCR-RFLP
Restriction Polymorphisms.
Bradyrhizobium
Rizobactérias
PCR-RFLP
Polimorfismo de restrição.
topic Bradyrhizobium
Rhizobacteria
PCR-RFLP
Restriction Polymorphisms.
Bradyrhizobium
Rizobactérias
PCR-RFLP
Polimorfismo de restrição.
description Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281
10.5433/1679-0359.2020v41n6Supl2p3067
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281
identifier_str_mv 10.5433/1679-0359.2020v41n6Supl2p3067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281/28262
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3067-3080
Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3067-3080
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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