Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281 |
Resumo: | Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus. |
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Semina. Ciências Agrárias (Online) |
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Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybeanCaracterização dos genes de cepas de Bradyrhizobium spp. contrastantes quanto à eficiência de fixação biológica de nitrogênio em sojaBradyrhizobiumRhizobacteriaPCR-RFLPRestriction Polymorphisms.BradyrhizobiumRizobactériasPCR-RFLPPolimorfismo de restrição.Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus.Bactérias do gênero Bradyrhizobium podem estabelecer simbiose com a soja e suprir a demanda de nitrogênio pela fixação biológica de nitrogênio (FBN). Este estudo teve como objetivo caracterizar genes relacionados à eficiência na FBN em cepas de B. japonicum contrastantes quanto a eficiência na FBN. As sequências gênicas relacionadas à eficiência da FBN em soja foram previamente identificadas na cepa S370 de B. japonicum utilizando uma técnica de DNA subtrativo. Os genes foram amplificados com iniciadores construídos a partir do genoma da cepa USDA 110. Os produtos de PCR foram digeridos com endonucleases de restrição e os produtos de RFLP foram analisados por eletroforese horizontal. Dos quatro genes estudados, apenas blr 3208 e blr 4511 amplificaram na maioria das cepas. Não foi observado polimorfismo no perfil de restrição dos genes blr 3208 e blr 4511 por PCR-RFLP. As cepas contrastantes tiveram os genes blr 3208 e blr 4511 sequenciados e a comparação das sequências nucleotídicas por análise de alinhamento múltiplo mostrou a presença de regiões internas preservadas, confirmando a análise realizada por PCR-RFLP. Os genes blr 3208 e blr 4511 são altamente conservados entre as cepas de B. japonicum, o que pode estar relacionado à função adaptativa durante o processo evolutivo do gênero Bradyrhizobium.UEL2020-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4028110.5433/1679-0359.2020v41n6Supl2p3067Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3067-3080Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3067-30801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281/28262Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMedeiros, Camila dePereira, Gilberto AguiarFreitas, Janyeli Dorini Silva deOliveira Filho, Olavo Bilac Quaresma deValle, Juliana Silveira doLinde, Giani AndreaPaccola-Meirelles, Luzia DorettoColauto, Nelson BarrosBarcellos, Fernando Gomes2022-10-04T16:31:27Zoai:ojs.pkp.sfu.ca:article/40281Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T16:31:27Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean Caracterização dos genes de cepas de Bradyrhizobium spp. contrastantes quanto à eficiência de fixação biológica de nitrogênio em soja |
title |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
spellingShingle |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean Medeiros, Camila de Bradyrhizobium Rhizobacteria PCR-RFLP Restriction Polymorphisms. Bradyrhizobium Rizobactérias PCR-RFLP Polimorfismo de restrição. |
title_short |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
title_full |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
title_fullStr |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
title_full_unstemmed |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
title_sort |
Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean |
author |
Medeiros, Camila de |
author_facet |
Medeiros, Camila de Pereira, Gilberto Aguiar Freitas, Janyeli Dorini Silva de Oliveira Filho, Olavo Bilac Quaresma de Valle, Juliana Silveira do Linde, Giani Andrea Paccola-Meirelles, Luzia Doretto Colauto, Nelson Barros Barcellos, Fernando Gomes |
author_role |
author |
author2 |
Pereira, Gilberto Aguiar Freitas, Janyeli Dorini Silva de Oliveira Filho, Olavo Bilac Quaresma de Valle, Juliana Silveira do Linde, Giani Andrea Paccola-Meirelles, Luzia Doretto Colauto, Nelson Barros Barcellos, Fernando Gomes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros, Camila de Pereira, Gilberto Aguiar Freitas, Janyeli Dorini Silva de Oliveira Filho, Olavo Bilac Quaresma de Valle, Juliana Silveira do Linde, Giani Andrea Paccola-Meirelles, Luzia Doretto Colauto, Nelson Barros Barcellos, Fernando Gomes |
dc.subject.por.fl_str_mv |
Bradyrhizobium Rhizobacteria PCR-RFLP Restriction Polymorphisms. Bradyrhizobium Rizobactérias PCR-RFLP Polimorfismo de restrição. |
topic |
Bradyrhizobium Rhizobacteria PCR-RFLP Restriction Polymorphisms. Bradyrhizobium Rizobactérias PCR-RFLP Polimorfismo de restrição. |
description |
Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281 10.5433/1679-0359.2020v41n6Supl2p3067 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281 |
identifier_str_mv |
10.5433/1679-0359.2020v41n6Supl2p3067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/40281/28262 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3067-3080 Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3067-3080 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306083280355328 |