Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls

Detalhes bibliográficos
Autor(a) principal: Oberst, Eneder Rosana
Data de Publicação: 2002
Outros Autores: Jobim, Maria Inês M., Cimarosti, Helena Iturvides, Souza, Diogo Onofre, Salbego, Christianne Gazzana, Wald, Vera Beatriz, Mattos, Rodrigo Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2063
Resumo: Two dimensional polyacrylamide gel electrophoresis was performed in seminal plasma of seven Bos taurus taurus and seven Bos taurus indicus bulls with high semen freezability, from an artificial insemination center. In a 8% polyacrylamide gels, three bands of 195, 66 and 55 kDa, present in 100% of the samples in both sub-species, were analyzed by their optical densities. In Bos taurus samples, the opticals densities of 55 kDa band, imunoidentified as osteopontin were superior (p < 0.05) than Bos indicus samples. The 66 kDa band was imunoidentified as albumin. In the Bos taurus samples, the bands did not show any variation among the bull samples, but in the Bos indicus, the albumin optical densities showed significant variation among samples.
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spelling Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine BullsImunoidentificação de Albumina e Osteopontina no Plasma Seminal de Reprodutores Taurinos e ZebuínosBovinesSeminal PlasmaProteinsBidimensional electrophoresis.BovinosPlasma seminalProteínasEletroforese bidimensional.Two dimensional polyacrylamide gel electrophoresis was performed in seminal plasma of seven Bos taurus taurus and seven Bos taurus indicus bulls with high semen freezability, from an artificial insemination center. In a 8% polyacrylamide gels, three bands of 195, 66 and 55 kDa, present in 100% of the samples in both sub-species, were analyzed by their optical densities. In Bos taurus samples, the opticals densities of 55 kDa band, imunoidentified as osteopontin were superior (p < 0.05) than Bos indicus samples. The 66 kDa band was imunoidentified as albumin. In the Bos taurus samples, the bands did not show any variation among the bull samples, but in the Bos indicus, the albumin optical densities showed significant variation among samples.As proteínas do plasma seminal de 14 reprodutores (7 Bos taurus taurus e 7 Bos taurus indicus), foram analisadas por eletroforese bidimensional, em géis de poliacrilamida a 8%, corados por Comassie Blue. Três bandas protéicas, presentes em 100% das amostras de plasma seminal, foram quantificadas de acordo com a densidade óptica exibida: 195 kDa, pI 6,5-7,5 ; 66 kDa, pI 5,4 e 55 kDa, pI 4,5. As amostras de plasma seminal provenientes de taurinos apresentaram densidades ópticas significativamente superiores (p &lt; 0,05) às dos zebuínos na bandaUEL2002-03-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/206310.5433/1679-0359.2002v23n1p21Semina: Ciências Agrárias; Vol. 23 No. 1 (2002); 21-28Semina: Ciências Agrárias; v. 23 n. 1 (2002); 21-281679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2063/1771Oberst, Eneder RosanaJobim, Maria Inês M.Cimarosti, Helena IturvidesSouza, Diogo OnofreSalbego, Christianne GazzanaWald, Vera BeatrizMattos, Rodrigo Costainfo:eu-repo/semantics/openAccess2011-03-24T22:00:11Zoai:ojs.pkp.sfu.ca:article/2063Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2011-03-24T22:00:11Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
Imunoidentificação de Albumina e Osteopontina no Plasma Seminal de Reprodutores Taurinos e Zebuínos
title Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
spellingShingle Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
Oberst, Eneder Rosana
Bovines
Seminal Plasma
Proteins
Bidimensional electrophoresis.
Bovinos
Plasma seminal
Proteínas
Eletroforese bidimensional.
title_short Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
title_full Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
title_fullStr Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
title_full_unstemmed Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
title_sort Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls
author Oberst, Eneder Rosana
author_facet Oberst, Eneder Rosana
Jobim, Maria Inês M.
Cimarosti, Helena Iturvides
Souza, Diogo Onofre
Salbego, Christianne Gazzana
Wald, Vera Beatriz
Mattos, Rodrigo Costa
author_role author
author2 Jobim, Maria Inês M.
Cimarosti, Helena Iturvides
Souza, Diogo Onofre
Salbego, Christianne Gazzana
Wald, Vera Beatriz
Mattos, Rodrigo Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oberst, Eneder Rosana
Jobim, Maria Inês M.
Cimarosti, Helena Iturvides
Souza, Diogo Onofre
Salbego, Christianne Gazzana
Wald, Vera Beatriz
Mattos, Rodrigo Costa
dc.subject.por.fl_str_mv Bovines
Seminal Plasma
Proteins
Bidimensional electrophoresis.
Bovinos
Plasma seminal
Proteínas
Eletroforese bidimensional.
topic Bovines
Seminal Plasma
Proteins
Bidimensional electrophoresis.
Bovinos
Plasma seminal
Proteínas
Eletroforese bidimensional.
description Two dimensional polyacrylamide gel electrophoresis was performed in seminal plasma of seven Bos taurus taurus and seven Bos taurus indicus bulls with high semen freezability, from an artificial insemination center. In a 8% polyacrylamide gels, three bands of 195, 66 and 55 kDa, present in 100% of the samples in both sub-species, were analyzed by their optical densities. In Bos taurus samples, the opticals densities of 55 kDa band, imunoidentified as osteopontin were superior (p < 0.05) than Bos indicus samples. The 66 kDa band was imunoidentified as albumin. In the Bos taurus samples, the bands did not show any variation among the bull samples, but in the Bos indicus, the albumin optical densities showed significant variation among samples.
publishDate 2002
dc.date.none.fl_str_mv 2002-03-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2063
10.5433/1679-0359.2002v23n1p21
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2063
identifier_str_mv 10.5433/1679-0359.2002v23n1p21
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2063/1771
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 23 No. 1 (2002); 21-28
Semina: Ciências Agrárias; v. 23 n. 1 (2002); 21-28
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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