Heparin-binding proteins of seminal plasma in Nellore bulls

Detalhes bibliográficos
Autor(a) principal: Fernandes,Carlos Eurico
Data de Publicação: 2009
Outros Autores: Souza,Fabiana Ferreira de, Souza-Neto,Jayme Augusto, Ribola,Paulo Eduardo Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
Resumo: The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.
id UFSM-2_06cec2ff0d194a07ae63ebf4f42ec551
oai_identifier_str oai:scielo:S0103-84782009000100048
network_acronym_str UFSM-2
network_name_str Ciência rural (Online)
repository_id_str
spelling Heparin-binding proteins of seminal plasma in Nellore bullsbovineproteinsseminal plasmaBos taurus indicusThe aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.Universidade Federal de Santa Maria2009-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048Ciência Rural v.39 n.1 2009reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782008005000044info:eu-repo/semantics/openAccessFernandes,Carlos EuricoSouza,Fabiana Ferreira deSouza-Neto,Jayme AugustoRibola,Paulo Eduardo Martinseng2009-01-09T00:00:00ZRevista
dc.title.none.fl_str_mv Heparin-binding proteins of seminal plasma in Nellore bulls
title Heparin-binding proteins of seminal plasma in Nellore bulls
spellingShingle Heparin-binding proteins of seminal plasma in Nellore bulls
Fernandes,Carlos Eurico
bovine
proteins
seminal plasma
Bos taurus indicus
title_short Heparin-binding proteins of seminal plasma in Nellore bulls
title_full Heparin-binding proteins of seminal plasma in Nellore bulls
title_fullStr Heparin-binding proteins of seminal plasma in Nellore bulls
title_full_unstemmed Heparin-binding proteins of seminal plasma in Nellore bulls
title_sort Heparin-binding proteins of seminal plasma in Nellore bulls
author Fernandes,Carlos Eurico
author_facet Fernandes,Carlos Eurico
Souza,Fabiana Ferreira de
Souza-Neto,Jayme Augusto
Ribola,Paulo Eduardo Martins
author_role author
author2 Souza,Fabiana Ferreira de
Souza-Neto,Jayme Augusto
Ribola,Paulo Eduardo Martins
author2_role author
author
author
dc.contributor.author.fl_str_mv Fernandes,Carlos Eurico
Souza,Fabiana Ferreira de
Souza-Neto,Jayme Augusto
Ribola,Paulo Eduardo Martins
dc.subject.por.fl_str_mv bovine
proteins
seminal plasma
Bos taurus indicus
topic bovine
proteins
seminal plasma
Bos taurus indicus
description The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.
publishDate 2009
dc.date.none.fl_str_mv 2009-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782008005000044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.39 n.1 2009
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140532234289152

Registros relacionados