Effects of insulin on sperm cell quality in ram semen cooled at 5°C
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43389 |
Resumo: | Insulin is present in the seminal plasma and is involved in sperm activities like motility and capacitation. However, the effects of insulin on the viability of cooled ram sperm are not fully understood. Therefore, the objective of the current study was to evaluate the effect of insulin addition on ram sperm maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates were divided into two aliquots with (insulin group) or without (control group) insulin (3 IU mL-1) in the semen extender, and then cooled at 5°C for 48 hours. Subsequently, the sperm cells were evaluated for motility and kinetics using computer-assisted semen analysis. The samples were evaluated for acrosomal integrity by fluorescein using isothiocyanate combined with peanut agglutinin (FITC-PNA) and membrane functionality by the hypoosmotic swelling test. The semen analysis was performed after 24 or 48 hours of cooling. There was an increased percentage of progressive sperm motility (%), straightness (%), linearity (%) and beat caudal frequency (Hz) in the insulin group after 24 and 48 hours of cooling (p < 0.05). However, insulin did not affect total sperm motility, sperm velocities (VSL, VAP and VCL) (µm seg-1), acrosomal integrity and membrane functionality during cooling (p > 0.05). In conclusion, the addition of 3 IU mL-1 insulin to ram semen extender improved the quality of sperm motility after cooling. |
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Semina. Ciências Agrárias (Online) |
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Effects of insulin on sperm cell quality in ram semen cooled at 5°CEfeitos da insulina sobre a qualidade das células espermáticas no sêmen ovino refrigerado a 5 oCCoolingGlucoseSemen extenderSheepSpermatozoa.Diluidor seminalEspermatozoidesGlicoseOvinos e Resfriamento.Insulin is present in the seminal plasma and is involved in sperm activities like motility and capacitation. However, the effects of insulin on the viability of cooled ram sperm are not fully understood. Therefore, the objective of the current study was to evaluate the effect of insulin addition on ram sperm maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates were divided into two aliquots with (insulin group) or without (control group) insulin (3 IU mL-1) in the semen extender, and then cooled at 5°C for 48 hours. Subsequently, the sperm cells were evaluated for motility and kinetics using computer-assisted semen analysis. The samples were evaluated for acrosomal integrity by fluorescein using isothiocyanate combined with peanut agglutinin (FITC-PNA) and membrane functionality by the hypoosmotic swelling test. The semen analysis was performed after 24 or 48 hours of cooling. There was an increased percentage of progressive sperm motility (%), straightness (%), linearity (%) and beat caudal frequency (Hz) in the insulin group after 24 and 48 hours of cooling (p < 0.05). However, insulin did not affect total sperm motility, sperm velocities (VSL, VAP and VCL) (µm seg-1), acrosomal integrity and membrane functionality during cooling (p > 0.05). In conclusion, the addition of 3 IU mL-1 insulin to ram semen extender improved the quality of sperm motility after cooling.A insulina está presente no plasma seminal e participa de atividades espermáticas, como a motilidade e capacitação. Entretanto, os efeitos da insulina sobre a viabilidade do espermatozoide ovino resfriado ainda não estão elucidadas. Desta forma, o objetivo do presente estudo foi avaliar os efeitos da adição de insulina sobre o espermatozoide ovino durante o tempo de armazenamento à 5º C. Amostras espermáticas de seis carneiros da raça Santa Inês foram utilizadas. Os ejaculados foram divididos em duas aliquotas, com (grupo insulina) ou sem (grupo controle) adição de insulina (3 UI mL-1) no diluidor seminal e, posteriormente, resfriados até 5oC e mantidos armazenados por 48 horas. Em seguida, os espermatozoides foram avaliados quanto a motilidade e cinética utilizando um Sistema Computadorizado de Análise de Sêmen (CASA). Adicionalmente, as amostras espermáticas foram analizadas quanto a integridade acrosomal por meio de sondas fluorescentes (FITC-PNA) e, funcionalidade de membrana pelo teste hiposmótico. As análises seminais foram realizadas após 24 ou 48 horas de resfriamento. Foram verificados aumentos de espermatozoides com motilidade progressiva (%), retilinearidade (%), linearidade (%) e frequência de batimento caudal (BCF) (Hz) no grupo insulina após 24 ou 48 horas de resfriamento (p < 0.05). Entretanto, não houve efeito da adição de insulina sobre a porcentagem de espermatozoides moveis (%) e das velocidades espermáticas (VSL, VAP e VCL) (µm seg-1), integridade acrossomal e funcionalidade de membrana durante o resfriamento (p > 0.05). Conclui-se que adição de insulina (3 UI mL-1) no diluidor seminal melhora a qualidade da motilidade espermática durante o resfriamento.UEL2021-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionIn vitro Sperm analysisapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4338910.5433/1679-0359.2021v42n6SUPL2p3803Semina: Ciências Agrárias; Vol. 42 No. 6SUPL2 (2021); 3803-3812Semina: Ciências Agrárias; v. 42 n. 6SUPL2 (2021); 3803-38121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43389/30366Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessvan Tilburg, Maurício FragaOliveira, Rodrigo Vasconcelos deGadelha, Carla Renata FigueiredoFagundes, BrunoDias, Angelo Jose BurlaQuirino, Célia RaquelSilva, José Frederico Straggiotti2022-09-29T16:39:39Zoai:ojs.pkp.sfu.ca:article/43389Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-29T16:39:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C Efeitos da insulina sobre a qualidade das células espermáticas no sêmen ovino refrigerado a 5 oC |
| title |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| spellingShingle |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C van Tilburg, Maurício Fraga Cooling Glucose Semen extender Sheep Spermatozoa. Diluidor seminal Espermatozoides Glicose Ovinos e Resfriamento. |
| title_short |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| title_full |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| title_fullStr |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| title_full_unstemmed |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| title_sort |
Effects of insulin on sperm cell quality in ram semen cooled at 5°C |
| author |
van Tilburg, Maurício Fraga |
| author_facet |
van Tilburg, Maurício Fraga Oliveira, Rodrigo Vasconcelos de Gadelha, Carla Renata Figueiredo Fagundes, Bruno Dias, Angelo Jose Burla Quirino, Célia Raquel Silva, José Frederico Straggiotti |
| author_role |
author |
| author2 |
Oliveira, Rodrigo Vasconcelos de Gadelha, Carla Renata Figueiredo Fagundes, Bruno Dias, Angelo Jose Burla Quirino, Célia Raquel Silva, José Frederico Straggiotti |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
van Tilburg, Maurício Fraga Oliveira, Rodrigo Vasconcelos de Gadelha, Carla Renata Figueiredo Fagundes, Bruno Dias, Angelo Jose Burla Quirino, Célia Raquel Silva, José Frederico Straggiotti |
| dc.subject.por.fl_str_mv |
Cooling Glucose Semen extender Sheep Spermatozoa. Diluidor seminal Espermatozoides Glicose Ovinos e Resfriamento. |
| topic |
Cooling Glucose Semen extender Sheep Spermatozoa. Diluidor seminal Espermatozoides Glicose Ovinos e Resfriamento. |
| description |
Insulin is present in the seminal plasma and is involved in sperm activities like motility and capacitation. However, the effects of insulin on the viability of cooled ram sperm are not fully understood. Therefore, the objective of the current study was to evaluate the effect of insulin addition on ram sperm maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates were divided into two aliquots with (insulin group) or without (control group) insulin (3 IU mL-1) in the semen extender, and then cooled at 5°C for 48 hours. Subsequently, the sperm cells were evaluated for motility and kinetics using computer-assisted semen analysis. The samples were evaluated for acrosomal integrity by fluorescein using isothiocyanate combined with peanut agglutinin (FITC-PNA) and membrane functionality by the hypoosmotic swelling test. The semen analysis was performed after 24 or 48 hours of cooling. There was an increased percentage of progressive sperm motility (%), straightness (%), linearity (%) and beat caudal frequency (Hz) in the insulin group after 24 and 48 hours of cooling (p < 0.05). However, insulin did not affect total sperm motility, sperm velocities (VSL, VAP and VCL) (µm seg-1), acrosomal integrity and membrane functionality during cooling (p > 0.05). In conclusion, the addition of 3 IU mL-1 insulin to ram semen extender improved the quality of sperm motility after cooling. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-10-08 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion In vitro Sperm analysis |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43389 10.5433/1679-0359.2021v42n6SUPL2p3803 |
| url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43389 |
| identifier_str_mv |
10.5433/1679-0359.2021v42n6SUPL2p3803 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/43389/30366 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
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Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 42 No. 6SUPL2 (2021); 3803-3812 Semina: Ciências Agrárias; v. 42 n. 6SUPL2 (2021); 3803-3812 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Universidade Estadual de Londrina (UEL) |
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UEL |
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UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1799306085377507328 |