Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106

Detalhes bibliográficos
Autor(a) principal: Krapiec, Priscila Vanessa
Data de Publicação: 2008
Outros Autores: Milaneze, Maria Auxiliadora, Machado, Maria de Fátima Pires da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2106
Resumo: The present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae) without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin) at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of growth regulators. The higher frequency of bud induction using BA and IBA combination indicated that this combinations were more effective for C. walkeriana in vitro multiplication using seedlings as explants
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spelling Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106Efeito de combinações diferentes de reguladores de crescimento a partir da indução de botões de plântulas de Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106orquídeasmicropropagaçãoCattleya walkerianareguladores de crescimento2.01.00.00-0 Biologia GeralThe present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae) without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin) at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of growth regulators. The higher frequency of bud induction using BA and IBA combination indicated that this combinations were more effective for C. walkeriana in vitro multiplication using seedlings as explantsNo presente estudo, foram investigadas diferentes combinações e concentrações de auxinas e citocininas para ativar o desenvolvimento de brotos e multiplicar plântulas de Cattleya walkeriana (Orchidaceae), sem estágio intermediário de calos. As plântulas de C. walkeriana foram cultivadas em meio B5, contendo 2% de sacarose, 0,65% de agar e combinações fatoriais das auxinas ácido naftalenoacético (NAA) e ácido 3-indolbutírico (IBA), e das citocininas 6-benzilaminopurina (BA) e cinetina (KIN), nas concentrações de 0,0; 0,5; 1,0 e 1,5mg/L. O maior número de brotos com folhas desenvolvidas foi formado nas plântulas cultivadas em meio contendo IBA e BA, enquanto o menor número de brotos foi observado na combinação NAA e KIN. Houve maior indução de brotos em meio contendo somente IBA (na ausência de citocininas) nas três concentrações. A maior freqüência de indução de brotos usando as combinações de BA e IBA indicaram que essa combinação foi mais efetiva para a multiplicação in vitro de C. walkeriana, usando plântulas como explantesUniversidade Estadual De Maringá2008-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/210610.4025/actascibiolsci.v25i1.2106Acta Scientiarum. Biological Sciences; Vol 25 No 1 (2003); 179-182Acta Scientiarum. Biological Sciences; v. 25 n. 1 (2003); 179-1821807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2106/1542Krapiec, Priscila VanessaMilaneze, Maria AuxiliadoraMachado, Maria de Fátima Pires da Silvainfo:eu-repo/semantics/openAccess2022-11-23T17:33:20Zoai:periodicos.uem.br/ojs:article/2106Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:20Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
Efeito de combinações diferentes de reguladores de crescimento a partir da indução de botões de plântulas de Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
title Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
spellingShingle Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
Krapiec, Priscila Vanessa
orquídeas
micropropagação
Cattleya walkeriana
reguladores de crescimento
2.01.00.00-0 Biologia Geral
title_short Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
title_full Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
title_fullStr Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
title_full_unstemmed Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
title_sort Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106
author Krapiec, Priscila Vanessa
author_facet Krapiec, Priscila Vanessa
Milaneze, Maria Auxiliadora
Machado, Maria de Fátima Pires da Silva
author_role author
author2 Milaneze, Maria Auxiliadora
Machado, Maria de Fátima Pires da Silva
author2_role author
author
dc.contributor.author.fl_str_mv Krapiec, Priscila Vanessa
Milaneze, Maria Auxiliadora
Machado, Maria de Fátima Pires da Silva
dc.subject.por.fl_str_mv orquídeas
micropropagação
Cattleya walkeriana
reguladores de crescimento
2.01.00.00-0 Biologia Geral
topic orquídeas
micropropagação
Cattleya walkeriana
reguladores de crescimento
2.01.00.00-0 Biologia Geral
description The present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae) without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin) at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of growth regulators. The higher frequency of bud induction using BA and IBA combination indicated that this combinations were more effective for C. walkeriana in vitro multiplication using seedlings as explants
publishDate 2008
dc.date.none.fl_str_mv 2008-04-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2106
10.4025/actascibiolsci.v25i1.2106
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2106
identifier_str_mv 10.4025/actascibiolsci.v25i1.2106
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2106/1542
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 25 No 1 (2003); 179-182
Acta Scientiarum. Biological Sciences; v. 25 n. 1 (2003); 179-182
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
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instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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